LOCUS       sequence-353424-        8296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of GCP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-GCP6
FEATURES             Location/Qualifiers
     source          1..8296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      6328..6337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7876..8010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7913..7932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7967..7986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8131..8296
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tggccagcat cacgcagctg ttcgacgacc tgtgtgaggc cctcctgccg
     2341 gctgccaaga ctcacctggg ccagcgcagt gtgaaccgga agagggcaaa gcggagcctc
     2401 aagaaggtgg cctacaatgc tcttttcaca aatctttttc aagatgagac tcaacagctg
     2461 cagcctgaca tgtcaaaact accagcgaga aacaagatcc tcatgttgtc ctttgacttg
     2521 agagtgggtg gcctgggccc caaggccgac cgtttggagg agcttgtgga ggagctggaa
     2581 gcagcccctt gctgtccgct tttggaggtg gggtctgttt tggacctcct ggttcagctg
     2641 gcagggagtg gtccccctca agttctgccg agaaaacgag actacttcct taacaacaag
     2701 catgtgggga gaaacgttcc gtacagcggc tatgattgcg acgacctgag tgtgtttgag
     2761 atggacgttc agtctctgat ctccagagaa gagtgtttgt gtcacagcat gatccaggaa
     2821 acacttcagg ttatggaggc tgctccaggc actggcctgc ccaccgtcgg gctcttctca
     2881 tttggtgacc cttgtggtga caggttcgag agagacaccc gggtctcgct cttcggggcc
     2941 cttgtgcaca gccgcactta tgacatggac gtccgactgg gcctgccccc cgtgccagac
     3001 aatgcggacc tctctgggct ggctattaag gtcccaccga gtgtggatca gtgggaagac
     3061 gaaggattcc agtcagcgtc caacttgact cctgattccc agtctgagcc cagcgtgacc
     3121 ccagacgtgg acctgtggga agccgcactt acctatgagg ccagcaagcg gaggtgctgg
     3181 gagcgagttg gctgcccccc tggccacaga gaggagcctt acctgacgga ggcgggaagg
     3241 gacgctttcg acaagttctg caggctccac caaggggagc tccagctgct tgctgggggc
     3301 gtcctacagg ccccgcagcc cgtgctggtg aaggagtgcg agctggtgaa agacgtgctg
     3361 aacgtcttga ttggggtcgt gtctgccacg ttttcgctct gccagccggc ccaggccttt
     3421 gtggtgaagc ggggcgtcca cgtgtcagga gcgtctcccg agagcatcag cagcctgctc
     3481 tcggaagtgg ccgagtatgg gacctgctac acgcgcctga gtcatttctc tctgcagccc
     3541 gtcctggact ctttgtacag caagggcctc gtgttccagg ccttcaccag tggcctgagg
     3601 aggtacctgc agtattaccg ggcctgcgtc ctttccactc cgcccaccct gagcctcctc
     3661 accattggtt ttctcttcaa gaaacttggc cggcagctca ggtacctggc cgagctctgt
     3721 ggcgttggcg ctgtgctccc gggcacctgt ggaggaggcc ccagggccgc gtttcccacc
     3781 ggcgtgaagc tgctgtccta cctctaccag gaggctctgc acaactgcag caacgagcac
     3841 taccctgtac tgctgtccct gctgaagacc agctgcgagc cctacacccg gttcatccac
     3901 gactgggtgt acagcggggt gttcagagac gcttatggcg agttcatgat tcaggtgaac
     3961 cacgagtacc tcagcttccg agataagttg tactggacac atggctacgt gctcatctca
     4021 aaagaggtgg aggactgtgt tcccgtgttt ctgaagcaca ttgcccacga catatacgtc
     4081 tgcggaaaga ccattaacct gctgaagctc tgctgccccc ggcattacct ctgttggtcc
     4141 gacgtccctg tcccccggat ctcggtgatt ttctcccttg aggagttgaa ggagattgag
     4201 aaggactgtg ccgtctacgt tgggcgcatg gagagggtgg cccgccacag ctctgtcagc
     4261 aaggaggaga aggaattacg tatggaaatt gcaaaacaag aattaatcgc tcatgcccgg
     4321 gaagcagcat ccagggtcct gagtgcactg agtgaccggc agatgtcaga acggatggcc
     4381 ttggatgccc ggaagcgtga gcagtttcag aggctgaaag aacaatttgt gaaggaccag
     4441 gagcgacgcc aggcggccag gcaggaggag ctggatgatg acttcagcta cgcccgtgaa
     4501 ctccgagaca gggagagaag gctgaagtcc ctggaggagg agctggagag gaaggcgagc
     4561 aagctctctg cagaggcagc tcgtcgggag cagaaggcac tgtggagaat ccagaggcac
     4621 cgactggaga gtgcacggct tcgttttctc ttagaagatg agaaacacat tcaggagatg
     4681 ctgaaagcag tgtctgaggc tcaccagccc caggagccgc cagacgtcct cttgagcgtg
     4741 cacccccagg tcacgtctcc gggccctgag cacccagagg gaggccaagg ctgtgattct
     4801 gggtctgcag agcaacactc gcctgcctgg gatggctgga acaggccagg cctgctgacc
     4861 ccacagcccc ttaagcctct agcagtgggg gctggtggca gggggctgca gcaggcggag
     4921 ggggccagac ccttctctga cagcctcagc attggagact tcctacctgt gggcccaggg
     4981 gctgagccgt ccgtgcagac tggcatggtc cctctcctgg aggtggcgct gcagacaatt
     5041 aacttggacc tgcccccctc agctcctggg gaggcacccg cagcagccag cactcagccc
     5101 tccaggccac aggagtacga ctttagtact gtcctgaggc cagctgtggc cacctcacct
     5161 gcaccagggc ccctgcaggc tgcagagtgc agcttgggca gctcagggct acagctgtgg
     5221 gaggacagtt gtgggaagat ggatgcctgt ggctccgcct cgcgggagac tctgctcccc
     5281 tcacacccac ccaggcgtgc tgctctggag gaggggagca gccagcccac agagcggctc
     5341 tttgggcagg tgtcaggggg tggtcttccc acaggggact acgcttctga aatagctccc
     5401 acccggccac ggtggaacac ccacgggcac gtgtctgacg ccagcatcag ggtcggggag
     5461 aatgtgtcag atgtggctcc cacccagcca cggtggaaca cccacggaca cgtatccaat
     5521 gccagcatca gcttagggga gtctgtgtca gatgtggctc ccactcggcc acggtggaac
     5581 atccatggac atgtgtccaa cgccagcatc agggtcgggg agaatgtgtc agatgtggct
     5641 cccaccaggc cacggtggaa tacccacggg cacgtgtcca acgccagcat cagggttggg
     5701 gagaacgtgt cggacgtggc tcccacccgg ccacggtgga acacccatgg acacgtgtcc
     5761 gatgccagca tcagcttggg ggagtctgtg tcagacatgg ctcccgcccg gccacggtgg
     5821 aacacccatg gacacgtgtc tgatgccagc atcagcttgg gggagtctgt gtcagacatg
     5881 gctcccaccc ggccacggtg gaacacccac ggacatgtgt ctgacaccag catcagggtt
     5941 ggggagaacg tatcggacgt ggctcccatc cggtcacggt gcaacaccca tggacacgtg
     6001 tccgacgcca gcatcagctt gggggagcct gtgtcggatg tggtttccac ccggccacgg
     6061 tggaacaccc atgtacccat ccctccgccc cacatggtgc tgggggctct ctcaccagaa
     6121 gctgagccca acacacccag gccccaacag agcccccctg gccacacgtc ccagtcagcg
     6181 ctcagcctgg gagcacagag cgctgtgctg gactgtgggc cacggctgcc tgtagaagtg
     6241 gggccatctc tgtcctcccc cagctcgggc tgcggggagg ggagcatcag cgtgggggag
     6301 aacgtgtcag acgtggctcc cacccaacca tggtggccca acaccccggg agacagtgtc
     6361 tctgaagaac taggccctgg gaggagcggg gacactgagg acctctctcc aaattggcct
     6421 ctcaactcac aggaagacac agctgcccag agcagcccag gccgtggtga ggaggcggag
     6481 gcatcggcgg cggaggctca gggtggggag caggcctacc tggcaggcct ggcagggcag
     6541 taccacttgg agcggtaccc ggacagttac gagtccatgt ccgagccgcc cattgctcat
     6601 cttttgcgcc ccgtgcttcc ccgggccttc gccttccccg tggaccccca ggtccagtct
     6661 gccgctgatg agactgctgt gcagctgagc gagttgctga cgctgcccgt gctcatgaag
     6721 cgctccatca cggcaccgct ggccgcccac atctccttgg tgaacaaggc cgctgtcgac
     6781 tacttcttcg tggagctgca cctggaggcg cactatgagg cactgcggca cttcctgctg
     6841 atggaggacg gcgagttcgc ccagtccctc agcgacctgc tctttgagaa gcttggagct
     6901 gggcaaacgc ccggagagct gctcaacccg ctggtgctga actctgtgct gagcaaggcc
     6961 ctgcagtgca gcctgcatgg ggacaccccg cacgcctcca acctctccct cgctctcaag
     7021 tacctgcccg aggtgtttgc ccccaacgcc ccggatgtgc tgagctgcct ggagctcagg
     7081 tacaaggtgg actggcctct caacattgtc atcaccgagg gctgcctgag caagtacagc
     7141 ggcgtcttct ccttcctgct gcagctgaag ctcatgatgt gggcgctcaa ggacgtctgc
     7201 ttccacctca agcgcacagc cctgctgagc cacatggccg gctctgtgca gttccgtcag
     7261 ctgcagctgt tcaagcacga gatgcagcat ttcgtgaagg tcatccaggg ctacatcgcc
     7321 aaccagatcc tgcacgtcac ctggtgcgag ttcagggcca ggttggccac cgtgggcgac
     7381 ctggaggaga tccagcgtgc gcacgcagag tacctgcacg aggccgtctt caggggcctg
     7441 ctcacggaga aggcggcgcc cgtcatgaac gtcatccaca gcatcttcag cctcgtgctc
     7501 aagttccgca gccagctcat ctcccaggcc tgggggcccc ctgggggccc gcggggtgca
     7561 gagcacccca actttgcact catgcagcag tcctacaaca ccttcaagta ctactcccac
     7621 tttctcttca aagtggtgac caagctggtg aaccgcggct accagcccca cctggaggac
     7681 tttctgctgc gcatcaactt caacaactac taccaggacg cctgatctag agcctgcagt
     7741 ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
     7801 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7861 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7981 ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc
     8041 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc
     8101 ataaccccta gggtataccc atctaattgg aaccagataa gtgaaatcta gttccaaact
     8161 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     8221 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     8281 attttgtccg cccaca
//
