LOCUS       sequence-353428-        5632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of GCP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-GCP2
FEATURES             Location/Qualifiers
     source          1..5632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2150..2167
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5212..5346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5249..5268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5303..5322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5467..5632
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc
     2281 gcggaattca tgagtgaatt tcggattcac catgacgtca atgaactgct tagcctgctg
     2341 cgtgtccacg gaggagatgg ggctgaggtc tacattgacc tgcttcaaaa gaacaggacc
     2401 ccgtacgtca ctaccactgt ctctgctcac agtgccaagg ttaaaattgc agagttttct
     2461 cgtactccag aagactttct aaagaaatat gatgaactga aatctaaaaa tacaaggaac
     2521 cttgacccgc tggtgtacct gttgtcaaag ctcacggaag acaaagagac tctgcagtac
     2581 ttacaacaga atgcaaaaga aagagctgag cttgcagccg ctgctgtggg cagcagtacc
     2641 accagcatca acgtccctgc cgcggcctcc aagatctcca tgcaggagct tgaggaactg
     2701 aggaagcagc ttggcagcgt ggccacaggc tccacgctgc agcagtctct ggaacttaaa
     2761 agaaagatgc ttcgagacaa gcagaacaaa aaaaattcag gccagcacct ccccatcttc
     2821 ccagcatggg tgtatgagag acctgccctg atcggggatt tcctgattgg tgctggcatc
     2881 agcacagaca ccgctttgcc gatagttctt ttgggatgga gtctcgctct gtcgcccagg
     2941 ctgaagtgca gtggcgtgat ctcggcacac tgcggcctcc acctcccagg cacgttgccc
     3001 ctggcctcgc aggagtcggc cgtggtggag gacctgctgt acgtgctggt gggcgtggac
     3061 gggaggtacg tcagtgctca gcccctggct gggaggcaga gccggacctt cctcgtggac
     3121 cccaacctgg acctgtccat cagggagctg gtgcacagga tcctcccagt ggccgccagc
     3181 tactccgctg tgaccaggtt cattgaagag aagtcttcct tcgagtacgg gcaggtgaac
     3241 cacgccctgg cggccgccat gcgcaccctg gtgaaggagc acctgattct ggtgtcacag
     3301 ctggagcagc tgcacaggca gggcctcctt tcgctgcaga agctctggtt ctacatccag
     3361 ccagccatgc gcaccatgga catcctggcc tccctcgcga cctcggtgga caaaggcgaa
     3421 tgtcttgggg ggtccacgct gagcctgctc cacgacagga gcttcagcta cacaggggac
     3481 agccaggcgc aggagctatg cctgtaccta accaaggcgg ccagtgctcc ctacttcgag
     3541 gttctggaga agtggatcta caggggcatc atccacgacc catacagtga gtttatggtc
     3601 gaggagcacg agctgcggaa ggagaggatc caggaggatt acaacgacaa gtactgggac
     3661 cagcggtaca ccatcgtcca gcagcagatc ccgtccttcc tgcagaaaat ggcggacaag
     3721 atcctcagca caggaaaata tctaaatgtg gtcagagagt gtggccatga cgtcacctgc
     3781 ccggtggcta aagagatcat ctacacgtta aaagagcggg cgtatgtgga gcagatcgag
     3841 aaggcgttta actacgccag caaggtgctg ctggacttcc tgatggagga gaaggagctg
     3901 gtggctcacc tcaggtccat caagcgctac ttcctcatgg accagggcga cttcttcgtg
     3961 cacttcatgg acctcgcgga ggaggagctc cggaagccgg tggaggacat cacgccccct
     4021 cgcctggaag cgctcctgga gctggcgctg cgcatgagca cggccaacac tgaccccttc
     4081 aaggacgacc tcaagatcga cctgatgccc catgacctca tcactcagct cttgcgcgtc
     4141 ctggccatcg agaccaagca ggagaaggcg atggcgcacg ccgacccgac ggagctggcg
     4201 ctgagcggcc tggaggcctt ctctttcgac tacatcgtca agtggcccct ttcgctcatc
     4261 atcaacagga aagccctcac tcgctaccag atgctcttca ggcacatgtt ctattgcaag
     4321 cacgtggagc ggcagctctg cagcgtctgg atcagcaaca aaaccgccaa gcagcactcg
     4381 ctgcactccg cccagtggtt tgctggggct ttcactctgc ggcagcgaat gctcaacttc
     4441 gtccagaata ttcaatacta catgatgttc gaagtgatgg aaccgacctg gcacatcctg
     4501 gagaaaaacc tgaaatccgc ctccaacatt gacgacgtcc ttggccacca cacaggcttc
     4561 ctggacacct gcctgaagga ctgcatgctc accaaccccg agctgctgaa ggtcttctcc
     4621 aagctcatgt ctgtgtgcgt catgttcacc aactgcatgc agaaatttac acagagcatg
     4681 aaattagatg gcgagctggg cgggcagacg ctggagcaca gcaccgtcct ggggctgccc
     4741 gcaggggccg aggagcgggc ccggaaggag ctcgccagga agcacctggc tgagcacgca
     4801 gacactgtgc agctggtgtc cggcttcgag gccaccatca acaagtttga caagaacttc
     4861 tcagcccacc tgctggacct cctggcccgg ctgagcatct atagcaccag tgactgtgag
     4921 cacggcatgg ccagcgtcat ctccaggctt gacttcaatg gtttctacac ggagcgcctg
     4981 gagcgcctgt ctgcagagag gagccagaag gccacccccc aagtgcctgt cctgcggggg
     5041 cccccggctc ctgcacccag ggtcgcagtc accgcacagt gaaagcttgt cgagaagtac
     5101 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     5161 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     5221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5341 tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc gaaaggaagc
     5401 tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt atacccatct
     5461 aattggaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     5521 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     5581 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca ca
//
