LOCUS       sequence-353432-        4843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of GCP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-GCP4
FEATURES             Location/Qualifiers
     source          1..4843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1906..1911,1912..1928,1929..1934))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2005..2038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2115..2206
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2131..2148
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4423..4557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4460..4479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4514..4533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4678..4843
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     1741 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     1801 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     1861 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     1921 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     1981 accttgggca gcagcgaagt cgccataact tcgtatagca tacattatac gaagttatct
     2041 gtaactataa cggtcctaag gtagcgagtt taaacactag tatcgattcg cgacctactc
     2101 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     2161 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     2221 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac
     2281 gtcgacgagc tcatgatcca cgaactgctc ttggctctga gcgggtaccc tgggtccatt
     2341 ttcacctgga acaagcggag tggcctgcag gtatcgcagg acttcccttt cctccacccc
     2401 agtgagacca gtgtcctgaa tcgactctgc cggctcggca cagactatat tcgcttcact
     2461 gagttcattg aacagtacac gggccatgtg caacagcagg atcaccatcc atctcaacag
     2521 ggccaaggtg ggttacatgg aatctacctg cgggccttct gcacagggct ggattctgtt
     2581 ttgcagcctt atcgccaagc actgcttgat ttggaacaag agttcctggg tgatccccat
     2641 ctctccatat cacatgtcaa ctacttccta gaccagttcc agcttctttt tccctctgtg
     2701 atggttgtag tagaacaaat taaaagtcaa aagattcatg gttgtcaaat cctggaaaca
     2761 gtctacaaac acagctgtgg ggggttgcct cctgttcgaa gtgcactgga aaaaatcctg
     2821 gccgtttgtc atggggtcat gtataaacag ctctcagcct ggatgctcca tggactcctc
     2881 ttggaccagc atgaagaatt ctttatcaaa caggggccat cttctggtaa tgtcagtgcc
     2941 cagccagaag aggacgagga ggatctgggc attgggggac tgacaggaaa acaactgaga
     3001 gaactgcagg acttgcgcct gattgaggaa gagaacatgc tggcaccatc tctgaagcag
     3061 ttttccctac gagtggagat tttgccatcc tacattccag tgagggttgc tgaaaaaatc
     3121 ctatttgttg gagaatctgt ccagatgttt gagaatcaaa atgtgaacct gactagaaaa
     3181 ggatccattt tgaaaaacca ggaagacact tttgctgcag agctgcaccg tctcaagcag
     3241 cagccactct tcagcttggt ggactttgaa caggtggtgg atcgcattcg cagcactgtg
     3301 gctgagcatc tctggaagtt gatggtagaa gaatccgatt tactgggtca gctgaagatc
     3361 attaaagact tttaccttct gggacgtgga gaactgtttc aggccttcat tgacacagct
     3421 caacacatgt tgaaaacacc acccactgca gtaactgagc atgatgtgaa tgtggccttt
     3481 caacagtcag cacacaaggt attgctagat gatgacaacc ttctccctct gttgcacttg
     3541 acaatcgagt atcacggaaa ggagcacaaa gatgctactc aggcaagaga agggccttct
     3601 cgggaaactt ctccccggga agcccctgca tctggctggg cagccctagg tctttcctac
     3661 aaagtacagt ggccactaca tattctcttc accccagctg tcctggaaaa gtacaatgtt
     3721 gtttttaagt acttactgag tgtgcgccgg gtgcaagctg agctgcagca ctgctgggcc
     3781 ctacaaatgc agcgcaagca cctcaagtcg aaccagactg atgcaatcaa gtggcgccta
     3841 agaaatcaca tggcattttt ggtggataat cttcagtact atctccaggt agatgtgttg
     3901 gagtctcagt tctcccagct gcttcatcag atcaattcta cccgagactt tgaaagcatc
     3961 cgattggctc atgaccactt cctgagcaat ttgctggctc aatcctttat cctattgaaa
     4021 cctgtgtttc actgcctgaa tgaaatccta gatctctgtc acagtttttg ttcgctggtc
     4081 agtcagaacc taggcccact ggatgagcgt ggagccgccc agctgagcat tctcgtgaag
     4141 ggctttagcc gccagtcttc actcctgttc aagattctct ccagtgttcg gaatcatcag
     4201 atcaactcag atttggctca actactgtta cgactagatt ataacaaata ctatacacag
     4261 gctggtggaa ctctgggcag tttcgggatg tgaaagcttg tcgagaagta ctagaggatc
     4321 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4381 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4441 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4501 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     4561 tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     4621 tgctgccacc gctgagcaat aactatcata acccctaggg tatacccatc taattggaac
     4681 cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac
     4741 gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc
     4801 catttccacc cctcccagtt cccaactatt ttgtccgccc aca
//
