LOCUS       sequence-353433-        4861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of NEDD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-NEDD1
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1906..1911,1912..1928,1929..1934))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2005..2038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2115..2206
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2131..2148
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4441..4575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4478..4497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4532..4551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4696..4861
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     1741 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     1801 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     1861 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     1921 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     1981 accttgggca gcagcgaagt cgccataact tcgtatagca tacattatac gaagttatct
     2041 gtaactataa cggtcctaag gtagcgagtt taaacactag tatcgattcg cgacctactc
     2101 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     2161 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     2221 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac
     2281 gtcgacatgc attttacagg cgcagtcatg caggaaaacc tcagatttgc ttcatcagga
     2341 gatgatatta aaatatggga tgcttcatct atgacattgg tggataaatt caacccacac
     2401 acatcaccac atggaatcag ctcaatatgt tggagcagca ataataactt tttagtaaca
     2461 gcatcttcca gtggcgacaa aatagttgtc tcaagttgca aatgtaaacc tgttccactt
     2521 ttagagcttg ctgaagggca aaagcagaca tgtgtcaatt taaattctac atctatgtat
     2581 ttggtaagcg gaggcctaaa taacactgtt aatatttggg atttaaaatc aaaaagagtt
     2641 catcgatctc ttaaggatca taaagatcaa gtaacttgtg taacatacaa ttggaatgat
     2701 tgctacattg cttctggatc tcttagtggt gaaattattt tacacagtgt aaccactaat
     2761 ttatctagta ctccttttgg ccatggtagt aaccagtctg ttcggcactt gaagtactcc
     2821 ttgtttaaga aatcactact gggcagtgtt tcggataatg gaatagtaac tctctgggat
     2881 gtaaatagtc agagtccata ccataacttt gacagtgtac acaaagctcc agcgtcaggc
     2941 atctgttttt ctcctgtcaa tgaattgctc tttgtaacta taggcttgga taaaagaatc
     3001 atcctctatg acacttcaag taagaagcta gtgaaaactt tagtggctga cactcctcta
     3061 actgcggtag atttcatgcc tgatggagcc actttggcta ttggatcttc ccgggggaaa
     3121 atatatcaat atgatttaag aatgttgaaa tcaccagtta agaccatcag tgctcacaag
     3181 acatctgtgc agtgtatagc atttcagtac tccactgttc ttactaagtc aagtttaaat
     3241 aaaggctgtt caaataagcc cacaacagtg aacaaacgaa gtgttaatgt gaatgctgct
     3301 agtggaggag ttcagaattc cggaattgtc agagaagcac ctgccacgtc cattgccaca
     3361 gttctaccac aacctatgac atcagctatg gggaaaggaa cagttgctgt tcaagaaaaa
     3421 gcaggtttgc ctcgaagcat aaacacagac actttatcta aggaaacaga cagtggaaaa
     3481 aatcaggatt tctccagctt tgatgatact gggaaaagta gtttaggtga catgttctca
     3541 cctatcagag atgatgctgt agttaacaag ggaagtgatg agtccatagg caaaggagat
     3601 ggctttgact ttctaccgca gttgaactca gtgtttcctc caagaaaaaa tccagtaact
     3661 tcaagtactt cagtattgca ttctagtcct cttaatgttt ttatgggatc tccagggaaa
     3721 gaggaaaatg aaaaccgtga tctaacagct gagtctaaga aaatatatat gggaaaacag
     3781 gaatctaaag actccttcaa acagttagca aagttggtca catctggtgc tgaaagtgga
     3841 aatctaaata cctctccatc atctaaccaa acaagaaatt ctgagaaatt tgaaaagcca
     3901 gagaatgaaa ttgaagccca gttgatatgt gaacccccaa tcaatggatc ctcaactcca
     3961 aatccaaaga tagcatcttc tgtcactgct ggagttgcca gttcactctc agaaaaaata
     4021 gccgacagca ttggaaataa ccggcaaaat gcaccattga cttccattca aattcgtttt
     4081 attcagaaca tgatacagga aacgttggat gactttagag aagcatgcca tagggacatt
     4141 gtgaatttgc aagtggagat gattaaacag tttcatatgc aactgaatga aatgcattct
     4201 ttgctggaaa gatactcagt gaatgaaggt ttagtggctg aaattgaaag actacgagaa
     4261 gaaaacaaaa gattacgggc ccacttttga tctagagcct gcagtctcga caagcttgtc
     4321 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4381 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4561 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
     4621 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac ccctagggta
     4681 tacccatcta attggaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     4741 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     4801 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     4861 a
//
