LOCUS       sequence-353437-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AG9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(244..249,250..266,267..272))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(399..987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(479..498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1392..1408
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1392..1408
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1413..1432
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1413..1431
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1606..2600,1..196))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2425..2444
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
       61 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      121 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      181 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      241 cgcattaaag ctaatcgatg ataagctgtc aaacatgaga attaattcat gttctttcct
      301 gcgttatccc ctgattctgt ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1021 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgaccag
     1081 atatggtctc aagaggcaat cgctacaact tgctccggag tcagaccgtg ggcttgacag
     1141 agaactggca gtaggcgctg tacggtttca agagcctgtt tgccaccatt gttactagca
     1201 atcgctacaa cttgatctgg ggtgagtccg tgggcttgac agagaactgg aaggaggcgc
     1261 tgaaccgtct caagagcctg tttgccacca atattggaac gagaccagct ttggtctaga
     1321 ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
     1381 gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatttt
     1441 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     1501 tccccgatca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1561 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1621 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1681 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1741 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     1801 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     1861 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     1921 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     1981 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2041 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2101 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2161 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2221 catgggtcgg cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2281 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgt aaggagatgg cgcccaacag
     2341 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2401 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2461 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag agaatccaca ggacgggtgt
     2521 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
     2581 gcggccaaag cggtcggaca
//
