LOCUS       sequence-353439-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AG10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     373..392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(599..604,605..621,622..627)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             675..1865
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1027..1046)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2039..2058)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2040..2058)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2063..2079)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2063..2079)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2484..2600,1..472)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
       61 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      541 tgcgttatcc acagaatcag gggataacgc aggaaagaac atgaattaat tctcatgttt
      601 gacagcttat catcgattag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
      661 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
      721 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
      781 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
      841 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
      901 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
      961 tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     1021 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     1081 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     1141 ccatctcctt acatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     1201 tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgacccatg cccttgagag
     1261 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1321 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1381 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1441 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     1501 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     1561 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     1621 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     1681 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca
     1741 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     1801 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     1861 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     1921 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tgatcgggga agaacagtat
     1981 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatatccc
     2041 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2101 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccaa
     2161 agctggtctc gctctaatat tggtggcaaa caggctcttg agacggttca gcgcctcctt
     2221 ccagttctct gtcaagccca cggactcacc ccagatcaag ttgtagcgat tgctagtaac
     2281 aatggtggca aacaggctct tgaaaccgta cagcgcctac tgccagttct ctgtcaagcc
     2341 cacggtctga ctccggagca agttgtagcg attgcgtcat gagaccatat ctggtcgagc
     2401 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2581 ctttttccga aggtaactgg
//
