LOCUS       sequence-353440-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2050
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3415..4003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3468..3488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3886..3897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4075..4308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4386..4507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4423..4442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4477..4496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4547..4682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4702..4721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4703..4721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4731..4748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4731..4747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4740..4762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4889..5344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4976..4995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5186..5207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5370..5474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5475..5543,5544..6335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5693..5712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6506..7094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6995..7014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7248..7265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7382..7403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7418..7423,7424..7441,7442..7448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7456..7472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7461..7483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7480..7496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7480..7496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7515..7535
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7517..7535
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7563..7789
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7790..7970
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8017..8142
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8635..8868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9053..9097
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9328..9538
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9350..9546
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9397..9532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9578..10180
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9578..9597)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10074..10094
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10223..10340
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ggagctgatg ctgctcagcg agattgctga cccgacgcgt ttcttcaccg acaacctgct
     1261 tagcccggag gactggggtc tgcagaacag caccttgtat tctggcctag atgaagtggc
     1321 cgaggagcag acgcagctct tccgttgccc ggagcaggat gtcccgtttg acggcagctc
     1381 cctggacgtg gggatggatg tcagcccctc tgagccccca tgggaactcc tgccgatctt
     1441 cccagatctt caggtgaagt ctgagccatc ttccccctgc tcttcctcct ccctcagctc
     1501 cgagtcatcg cgtctctcca cagagccatc cagcgaggct cttggggtag gggaggtgct
     1561 ccatgtgaag acagagtcct tggcaccccc actgtgtctc ctgggagatg acccaacatc
     1621 ctcatttgaa accgtccaga tcaacgttat ccccacctct gatgattcct cagatgtcca
     1681 gaccaagata gaacctgtct ctccatgttc ttccgtcaac tctgaggcct ccctgctctc
     1741 agccgactcc tccagccagg cttttatagg agaggaggtc ctggaagtga agacagagtc
     1801 cctgtcccct tcaggatgcc tcctgtggga tgtcccagcc ccctcacttg gagctgtcca
     1861 gatcagcatg ggcccatccc ttgatggctc ctcaggcaaa gccctgccca cccggaagcc
     1921 gccactgcag cccaaacctg tagtgctaac cactgtccca atgccatcca gagctgtgcc
     1981 tcccagcacc acagtccttc tgcagtccct cgtccagcca cccccagtgt ccccagttgt
     2041 cctcatccag ggtgctattc gagtccagcc tgaagggccg gctccctctc taccacggcc
     2101 tgagaggaag agcatcgttc ccgctcctat gcctggaaac tcctgcccgc ctgaagtgga
     2161 tgcaaagctg ctgaagcggc agcagcgaat gatcaagaac cgggagtcag cctgccagtc
     2221 ccggagaaag aagaaagagt atctgcaggg actggaggct cggctgcaag cagtactggc
     2281 tgacaaccag cagctccgcc gagagaatgc tgccctccgg cggcggctgg aggccctgct
     2341 ggctgaaaac agcgagctca agttagggtc tggaaacagg aaggtggtct gcatcatggt
     2401 cttccttctc ttcattgcct tcaactttgg acctgtcagc atcagtgagc ctccttcagc
     2461 tcccatctct cctcggatga acaaggggga gcctcaaccc cggagacact tgctggggtt
     2521 ctcagagcaa gagccagttc agggagttga acctctccag gggtcctccc agggccctaa
     2581 ggagccccag cccagcccca cagaccagcc cagtttcagc aacctgacag ccttccctgg
     2641 gggcgccaag gagctactac taagagacct agaccagctc ttcctctcct ctgattgccg
     2701 gcacttcaac cgcactgagt ccctgaggct tgctgacgag ttgagtggct gggtccagcg
     2761 ccaccagaga ggccggagga agatccctca gagggcccag gagagacaga agtctcagcc
     2821 acggaagaag tcacctccag ttaaggcagt ccccatccaa ccccctggac ccccagaaag
     2881 ggattctgtg ggccagctgc aactatatcg ccacccagac cgttcgcagc cagcattctt
     2941 ggatgcaatt gaccgacggg aagacacatt ttatgttgtc tctttccgaa gggaccacct
     3001 gctgctccca gccatcagcc acaacaagac ctcccggccc aagatgtccc tggtgatgcc
     3061 tgccatggcc cccaatgaga ccctgtcagg ccgtggggcc ccgggggact atgaggagat
     3121 gatgcagatc gagtgtgagg tcatggacac cagggtgatt cacatcaaga cctccacagt
     3181 gcccccctcg ctccgaaaac agccatcccc aaccccaggc aatgccacag gtggcccctt
     3241 gccagtctct gcagccagcc aggcccacca ggcctcccac cagcccctct acctcaatca
     3301 tccctagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     3361 ttgtggaaag gacgagttca attccctagt gcggcactta cgcgttaagt cgacaatcaa
     3421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3781 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     4021 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4081 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4141 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4201 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4261 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     4321 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4381 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4501 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4681 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4741 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4801 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4861 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5101 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5221 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5281 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5341 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5461 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5521 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5581 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5641 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5701 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5761 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5821 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5881 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5941 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6001 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6061 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7261 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7441 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7501 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     7561 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7621 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7681 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7741 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7981 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8041 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8101 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8161 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8221 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8281 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8341 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8401 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8461 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8521 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8581 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8641 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8701 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8761 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8821 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8881 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8941 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9001 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9061 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9121 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9181 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9241 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     9301 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     9361 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9421 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9481 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9541 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     9601 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     9661 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9721 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9781 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9841 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9901 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9961 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    10021 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    10081 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    10141 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    10201 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10261 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10321 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    10381 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    10441 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
