LOCUS       sequence-353441-       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2052
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3256..3844
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3309..3329)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3727..3738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3916..4149
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4227..4348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4264..4283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4318..4337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4388..4523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4543..4562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4544..4562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4572..4589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4572..4588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4581..4603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4730..5185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4817..4836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5027..5048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5211..5315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5316..5384,5385..6176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5534..5553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6347..6935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6836..6855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7089..7106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7223..7244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7259..7264,7265..7282,7283..7289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7297..7313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7302..7324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7321..7337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7321..7337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7356..7376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7358..7376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7404..7630
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7631..7811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7858..7983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8476..8709
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8894..8938
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9169..9379
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9191..9387
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9238..9373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9419..10021
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9419..9438)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9915..9935
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10064..10181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ctcgccggct gacagctgta tccagttcac ccgccatgcc agtgatgttc ttctcaacct
     1261 taatcgtctc cggagtcgag acatcttgac tgatgttgtc attgttgtga gccgtgagca
     1321 gtttagagcc cataaaacgg tcctcatggc ctgcagtggc ctgttctata gcatctttac
     1381 agaccagttg aaatgcaacc ttagtgtgat caatctagat cctgagatca accctgaggg
     1441 attctgcatc ctcctggact tcatgtacac atctcggctc aatttgcggg agggcaacat
     1501 catggctgtg atggccacgg ctatgtacct gcagatggag catgttgtgg acacttgccg
     1561 gaagtttatt aaggccagtg aagcagagat ggtttctgcc atcaagcctc ctcgtgaaga
     1621 gttcctcaac agccggatgc tgatgcccca agacatcatg gcctatcggg gtcgtgaggt
     1681 ggtggagaac aacctgccac tgaggagcgc ccctgggtgt gagagcagag cctttgcccc
     1741 cagcctgtac agtggcctgt ccacaccgcc agcctcttat tccatgtaca gccacctccc
     1801 tgtcagcagc ctcctcttct ccgatgagga gtttcgggat gtccggatgc ctgtggccaa
     1861 ccccttcccc aaggagcggg cactcccatg tgatagtgcc aggccagtcc ctggtgagta
     1921 cagccggccg actttggagg tgtcccccaa tgtgtgccac agcaatatct attcacccaa
     1981 ggaaacaatc ccagaagagg cacgaagtga tatgcactac agtgtggctg agggcctcaa
     2041 acctgctgcc ccctcagccc gaaatgcccc ctacttccct tgtgacaagg ccagcaaaga
     2101 agaagagaga ccctcctcgg aagatgagat tgccctgcat ttcgagcccc ccaatgcacc
     2161 cctgaaccgg aagggtctgg ttagtccaca gagcccccag aaatctgact gccagcccaa
     2221 ctcgcccaca gagtcctgca gcagtaagaa tgcctgcatc ctccaggctt ctggctcccc
     2281 tccagccaag agccccactg accccaaagc ctgcaactgg aagaaataca agttcatcgt
     2341 gctcaacagc ctcaaccaga atgccaaacc agaggggcct gagcaggctg agctgggccg
     2401 cctttcccca cgagcctaca cggccccacc tgcctgccag ccacccatgg agcctgagaa
     2461 ccttgacctc cagtccccaa ccaagctgag tgccagcggg gaggactcca ccatcccaca
     2521 agccagccgg ctcaataaca tcgttaacag gtccatgacg ggctctcccc gcagcagcag
     2581 cgagagccac tcaccactct acatgcaccc cccgaagtgc acgtcctgcg gctctcagtc
     2641 cccacagcat gcagagatgt gcctccacac cgctggcccc acgttccctg aggagatggg
     2701 agagacccag tctgagtact cagattctag ctgtggtgag aaaccctatc gttgcaacat
     2761 ctgtggggcc cagttcaacc ggccagccaa cctgaaaacc cacactcgaa ttcactctgg
     2821 agagaagccc tacaaatgcg aaacctgcgg agccagattt gtacaggtgg cccacctccg
     2881 tgcccatgtg cttatccaca ctggtgagaa gccctatccc tgtgaaatct gtggcacccg
     2941 tttccggcac cttcagactc tgaagagcca cctgcgaatc cacacaggag agaaacctta
     3001 ccattgtgag aagtgtaacc tgcatttccg tcacaaaagc cagctgcgac ttcacttgcg
     3061 ccagaagcat ggcgccatca ccaacaccaa ggtgcaatac cgcgtgtcag ccactgacct
     3121 gcctccggag ctccccaaag cctgctagta atgaactagt ccgtaacttg aaagtatttc
     3181 gatttcttgg ctttatatat cttgtggaaa ggacgagttt aagctgggtt ggccagtatt
     3241 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3301 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3361 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3421 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3481 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3541 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3601 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3661 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3721 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3781 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3841 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3901 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3961 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4021 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4081 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4141 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4201 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4321 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4381 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4441 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4501 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4561 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4621 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5161 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7321 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7381 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7441 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7501 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7561 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7621 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7681 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7741 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7801 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7861 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7921 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7981 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     8041 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8101 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     8161 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     8221 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     8281 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     8341 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8401 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8461 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8521 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8581 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8641 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8701 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8761 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8821 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8881 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8941 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9001 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9061 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     9121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9181 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9301 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9361 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     9421 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9481 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9541 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9601 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9661 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9721 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9781 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9841 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9901 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9961 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
    10021 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    10081 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10141 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10201 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
    10261 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
