LOCUS       sequence-35345-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Park IH, Chen J
  TITLE     Mammalian target of rapamycin (mTOR) signaling is required for a 
            late-stage fusion process during skeletal myotube maturation.
  JOURNAL   J Biol Chem. 2005 Sep 9. 280(36):32009-17.
  PUBMED    16043480
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Sep 9. 280(36):32009-17."
COMMENT     Sequence Label: pIRESneo-mTOR-RR/KI
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcatagcgac tctctggcta ctgagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tcgatatctg cggccgccat
      121 ggattataaa gatgatgatg atatgcttgg aaccggacct gccgccgcca ccaccgctgc
      181 caccacatct agcaatgtga gcgtcctgca gcagtttgcc agtggcctaa agagccggaa
      241 tgaggaaacc agggccaaag ccgccaagga gctccagcac tatgtcacca tggaactccg
      301 agagatgagt caagaggagt ctactcgctt ctatgaccaa ctgaaccatc acatttttga
      361 attggtttcc agctcagatg ccaatgagag gaaaggtggc atcttggcca tagctagcct
      421 cataggagtg gaaggtggga atgccacccg aattggcaga tttgccaact atcttcggaa
      481 cctcctcccc tccaatgacc cagttgtcat ggaaatggca tccaaggcca ttggccgtct
      541 tgccatggca ggggacactt ttaccgctga gtacgtggaa tttgaggtga agcgagccct
      601 ggaatggctg ggtgctgacc gcaatgaggg ccggagacat gcagctgtcc tggttctccg
      661 tgagctggcc atcagcgtcc ctaccttctt cttccagcaa gtgcaaccct tctttgacaa
      721 catttttgtg gccgtgtggg accccaaaca ggccatccgt gagggagctg tagccgccct
      781 tcgtgcctgt ctgattctca caacccagcg tgagccgaag gagatgcaga agcctcagtg
      841 gtacaggcac acatttgaag aagcagagaa ggga
//
