LOCUS       sequence-35346-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Sin3A and Sin3B Plasmids
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pME18S-HismSin3A
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             51..319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          349..445
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             496..525
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             541..555
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cctcgatcga ggggctcgca
       61 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      121 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      181 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      241 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      301 ttctgcgccg ttacagatcc aagctctgaa aaaccagaaa gttaactggt aagtttagtc
      361 tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag aactgctcct
      421 cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc tctaaaagct
      481 gcggaattca tgggccatca tcatcatcat catcatcatc atcacagcag cggccatatc
      541 gacgacgacg acaagcatat gctcgagaag cgacggttgg atgaccagga atcaccagtg
      601 tatgcagccc agcagcgaag gattcctggg agcacagagg ctttttctca ccagcaccgg
      661 gtccttgccc cggcccctcc tgtgtatgaa gcagtgtctg agaccatgca gtcagctaca
      721 ggcattcagt actcagtggc acccaactac caggtttcag ctgtgccaca aagttctggc
      781 agtcatgggc ccgccatagc agcagttcat agcagccatc atcacccaac agctgtcc
//
