LOCUS       sequence-353469-        8710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP 
            cassette and Venus (also see doi: 10.7554/eLife.63907 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Yamagata M, Sanes JR
  TITLE     CRISPR-mediated Labeling of Cells in Chick Embryos Based on 
            Selectively Expressed Genes.
  JOURNAL   Bio Protoc. 2021 Aug 5;11(15):e4105. doi: 10.21769/BioProtoc.4105. 
            eCollection 2021 Aug 5.
  PUBMED    34458399
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2021-08-5"; volume: "11(15):e4105. doi"; pages: " 
            10.21769/BioProtoc.4105. eCollection 2021 Aug 5"
COMMENT     Sequence Label: pXL-BacII-CAG-loxP-STOP-loxP-Venus
FEATURES             Location/Qualifiers
     source          1..8710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..584
                     /label=chicken beta-actin promoter
     intron          585..1601
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1525..1547
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1609..1628
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1668..1692
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1749..1782)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2269..2334
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2464..2484
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2909..3043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2946..2965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3000..3019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3085..3118)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3285..3287,3288..3290,3291..4004)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3330..3351)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3591..3610)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3938..3959
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4108..4132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4223..4242)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4288..4343
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4342..4361)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   4966..5000
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5491..5507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5491..5507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5504..5526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5515..5531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5539..5545,5546..5563,5564..5569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5584..5605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5722..5739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5893..6481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5973..5992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6652..7443,7444..7512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7275..7294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7513..7617)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7643..8098)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7780..7801)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7992..8011
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8225..8247
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8239..8256
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8240..8256
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8386..8448
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggggggggc gcgcccgggg gggggggggg gggggggggg ggggggcggg gcgaggcgga
      481 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      541 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      601 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      661 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      721 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      781 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      841 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      901 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
      961 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1021 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1081 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1141 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     1201 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1261 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1321 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1381 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1441 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1501 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1561 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1621 ttattgtgct gtctcatcat tttggcaaag aattcctcga ggatatcaca agtttgtaca
     1681 aaaaagcagg ctccgaattc gcccttcgta actataacgg tcctaaggta gcgaattcac
     1741 cggtcctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttccggatcc
     1801 tcggggacac caaatagcta gcggcgcgcc ctctcttaag gtagcagatc tgctgccacc
     1861 gttgtttcca ccgaagaaac caccgttgcc gtaaccacca cgacggttgt tgctaaagaa
     1921 gctgccaccg ccacggccac cgttgtagcc gccgttgttg ttattgtagt tgctactgtt
     1981 atttctggca cttcttggtt ttcctcttaa gtgaggagga acataaccat tctcgttgtt
     2041 gtcgttgatg cttaaatttt gcacttgttc gctcagttca gccataatat gaaatgcttt
     2101 tcttgttgtt cttacggaat accacttgcc acctatcacc acaactaact ttttcccgtt
     2161 cctccatctc ttttatattt tttttctcga gggatctttg tgaaggaacc ttacttctgt
     2221 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     2281 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     2341 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     2401 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     2461 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     2521 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     2581 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     2641 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     2701 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     2761 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     2821 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2881 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3001 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgacatg gtaagtaagc
     3061 ttgggctgca ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat
     3121 taagggtacc ggttctagag cggcctcgac tcgagcgtaa ctataacggt cctaaggtag
     3181 cgaatagtga accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
     3241 gaattctgca gtcgacggta ccgcgggccc gggatcgatc caccatggtg agcaagggcg
     3301 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3361 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3421 agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg
     3481 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3541 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3601 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3661 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3721 acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     3781 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     3841 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     3901 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3961 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgagcggcc gcgactctag
     4021 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     4081 ctccccctga acctaagggc gaattcgacc cagctttctt gtacaaagtg gtgattcgcg
     4141 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4201 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4261 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4321 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4381 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     4441 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4501 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     4561 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     4621 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     4681 agcccaagct tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     4741 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     4801 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     4861 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     4921 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     4981 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     5041 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     5101 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     5161 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     5221 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     5281 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     5341 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     5401 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     5461 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5581 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5641 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5701 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6061 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6301 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7621 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     7681 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     7741 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     7801 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     7861 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     7921 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     7981 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     8041 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     8101 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8161 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8221 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt
     8281 tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag cgtgatggag
     8341 cagatgaaga tgctcgacac gctgcagaac acgcagctag attaacccta gaaagataat
     8401 catattgtga cgtacgttaa agataatcat gtgtaaaatt gacgcatgtg ttttatcggt
     8461 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
     8521 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
     8581 acaaaaactc aaaatttctt ctataaagta acaaaacttt tatcgaattc ctgcagcccg
     8641 ggggatccac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8701 tggagttccg
//
