LOCUS       sequence-353488-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector to express sgRNA resistant mouse Atg7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Zhu XG, Chudnovskiy A, Baudrier L, Prizer B, Liu Y, Ostendorf BN, 
            Yamaguchi N, Arab A, Tavora B, Timson R, Heissel S, de Stanchina E, 
            Molina H, Victora GD, Goodarzi H, Birsoy K
  TITLE     Functional Genomics In Vivo Reveal Metabolic Dependencies of 
            Pancreatic Cancer Cells.
  JOURNAL   Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30550-7. doi: 
            10.1016/j.cmet.2020.10.017.
  PUBMED    33152324
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2020 Oct 28. pii: S1550-4131(20)30550-7. doi: 
            10.1016/j.cmet.2020.10.017."
COMMENT     Sequence Label: pMXS-IRES-Blast mAtg7
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(4173..4747,4748..4750,4751..4759)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4340..4357)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4567..4586
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4760..5155
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5326..5795
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5925..5942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6096..6684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6176..6195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6848..7639,7640..7708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7471..7490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7709..7813)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaagcca
     1921 ccatgcatac aggcctctgg aaaattccca cgagcacagt ggtgaggcca actccacact
     1981 gctttcctgc catgaggctt tcctcatctg gatgtgagca cctgacagtg aggctggtaa
     2041 gaacagtagc catgggggac cctggactgg ccaagttgca gttcgccccc tttaatagtg
     2101 ccctggacgt tggcttctgg cacgaactga cccagaagaa actcaatgaa tatagactag
     2161 acgaggcacc caaagacatc aagggctatt actacaatgg tgactctgct ggtctgccca
     2221 cccgcttgac gttggagttc agtgcttttg acatgagtgc ctccacgcct gcccactgct
     2281 gcccggccat gggaaccctg cacaacacca acacacttga ggcttttaag acagcagaca
     2341 agaagctcct tctggagcag tcagcaaatg agatctggga agccataaag tcaggtgctg
     2401 ctctcgaaaa cccaatgtta ttgaataaat tcctactcct gaccttcgcg gacctaaaga
     2461 agtaccactt ctactactgg ttttgctgcc ccgccctctg tcttcctgag agcatccctc
     2521 taatccgggg acctgtgagc ttggatcaaa ggctttcacc aaaacagatc caggccctgg
     2581 agcatgccta tgatgatctg tgtcgagccg aaggcgtcac ggccctgccc tacttcttat
     2641 tcaagtacga tgacgacact gttctggtct ccttgctcaa acactacagt gatttcttcc
     2701 aaggtcaaag gacaaagata acagttggtg tgtacgatcc ctgtaaccta gcccagtacc
     2761 ctggatggcc tttgaggaat tttttggtcc tggcagccca cagatggagc ggcagtttcc
     2821 agtccgttga agtcctctgc tttcgggacc gcaccatgca gggagctaga gacgtgacac
     2881 atagcatcat ctttgaagtg aaacttccag aaatggcatt tagcccagat tgtcctaaag
     2941 ctgttggctg ggagaagaac cagaaaggag gcatgggtcc gaggatggtg aacctcagtg
     3001 gatgtatgga ccccaaaagg ctggctgagt catctgtgga tctgaatctc aagctgatgt
     3061 gctggcgatt ggtccccacc ttggacttgg acaaggtcgt gtctgtcaag tgcctgctgc
     3121 tgggagctgg taccttgggg tgtaatgtgg ctaggacact gatgggctgg ggcgtcagac
     3181 atgtcacctt tgtggataac gccaagatct cctactccaa tcccgtgagg cagcctctgt
     3241 atgaatttga agattgtcta gggggtggca agcccaaggc cctggctgca gcagagcggc
     3301 tacagaaaat atttcccgga gtgaatgcca gagggttcaa catgagcatc cccatgccag
     3361 gacaccctgt gaacttctct gacgtcacta tggaacaagc ccgcagagat gtggagcagc
     3421 tggagcagct cattgataac catgatgtca tcttcctgct aatggacacc agggagagcc
     3481 ggtggcttcc tactgttatt gcagccagca agcgaaagct ggtcatcaac gctgccttgg
     3541 ggtttgatac ctttgttgtc atgagacatg gcctgaagaa acccaagcag cagggagccg
     3601 gagacctctg cccaagccat cttgtagcac ctgctgacct gggctcctca ctttttgcca
     3661 acatccctgg atacaagctt ggctgctact tctgcaatga tgtggtggct ccaggagatt
     3721 caaccagaga ccggactctg gaccagcagt gcacagtgag ccgcccaggc ctggccgtga
     3781 ttgcaggtgc cctggctgtg gagctgatgg tctctgtcct gcagcatcct gaggggggct
     3841 acgccatcgc cagcagcagt gatgaccgca tgaatgagcc tcccacctcg ctgggacttg
     3901 tgcctcacca gatccggggt tttctgtcac ggttcgataa tgttcttcct gtcagcctgg
     3961 catttgataa atgtacagcc tgttcaccca aagttcttga tcagtacgag cgagaaggat
     4021 tcaccttcct agcgaaggtt tttaactcct cacattcctt cttagaagac ttgaccggtc
     4081 ttactctatt acaccaagag acccaagctg ctgagatctg ggacatgagt gacgaggaga
     4141 ctgtctgagc tacgtaaatt ccgccccccc cccccctctc cctccccccc ccctaacgtt
     4201 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4261 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4321 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4381 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4441 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4501 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4561 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4621 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4681 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4741 tgataatatg gccacaacca tggttccttt gtctcaagaa gaatccaccc tcattgaaag
     4801 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     4861 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     4921 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     4981 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     5041 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     5101 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagtcga
     5161 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     5221 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     5281 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     5341 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     5401 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5461 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     5521 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     5581 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5641 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     5701 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5761 tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt
     5821 tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa
     5881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6781 acctagatcc ttttgcggcc ggccgcaaat caatctaaag tatatatgag taaacttggt
     6841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7801 taggggttcc gcgcacattt c
//
