LOCUS       sequence-353490-       15124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Harant A, Pai H, Sakai T, Kamoun S, Adachi H
  TITLE     A vector system for fast-forward studies of the HOPZ-ACTIVATED 
            RESISTANCE1 (ZAR1) resistosome in the model plant Nicotiana 
            benthamiana.
  JOURNAL   Plant Physiol. 2022 Jan 20;188(1):70-80. doi: 
            10.1093/plphys/kiab471.
  PUBMED    34633454
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2022-01-20- 20"; volume: "188"; issue: "1"; 
            pages: "70-80"
COMMENT     Sequence Label: pZA70
FEATURES             Location/Qualifiers
     source          1..15124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        409..753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     697..717
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          1209..2805
                     /gene="pdk"
                     /number=3
                     /label=PDK intron
                     /note="modified pyruvate orthophosphate dikinase intron 
                     from Flaveria trinervia"
     CDS             1547..2230
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     terminator      3144..3396
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3405..3422)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3405..3421)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3414..3436)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3561..3580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3771..3792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4039..4063
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(5659..6807)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7106..7900)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGGADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  8092..8859
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      9482..10193
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10320..10691)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10724..10833
                     /note="incP origin of transfer"
     primer_bind     11056..11075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11309..11326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(11514..12164)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    12629..12653
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        12777..12956
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        12809..12992
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             13013..13807
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13067..13086)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13677..13696
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      14197..14449
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    14998..15019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15034..15039,15040..15057,15058..15064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15072..15088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15077..15099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15096..15112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15096..15112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctttcaa catgtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
       61 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      121 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      181 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga
      241 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
      301 aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg acacacttgt
      361 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
      421 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
      481 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacgtcgaga gttctcaaca caacatatac
      781 aaaacaaacg aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt
      841 tcttttaaag caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tagccagggc
      901 ccggaggcac tagtgtatga agatgccaga tttgagactc ttgctaattt ccttgacccc
      961 aattgcgata aattgttaag ttggaaatgt agattgaaga ttgccaaaag cattgccagt
     1021 gcaattctct atctacacac tgcatttcca actcccatta tctacagaat cttgaacccc
     1081 cataacatta tcttggacca tcattgcgtt cccaaactgt ttgatttctc gtttgttatt
     1141 tctcttcctc ctggagagtt gaaagtggaa gatgacttga tttggattcc tggctatacg
     1201 agcccttggt aaggaaataa ttattttctt ttttcctttt agtataaaat agttaagtga
     1261 tgttaattag tatgattata ataatatagt tgttataatt gtgaaaaaat aatttataaa
     1321 tatattgttt acataaacaa catagtaatg taaaaaaata tgacaagtga tgtgtaagac
     1381 gaagaagata aaagttgaga gtaagtatat tatttttaat gaatttgatc gaacatgtaa
     1441 gatgatatac ggccgaagag gttccaactt tcaccataat gaaataagat cactaccggg
     1501 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     1561 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     1621 tcagtcagtt gttcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     1681 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1741 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
     1801 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1861 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1921 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1981 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2041 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     2101 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     2161 taatgaatta caacagtact gcgatgagtg gcaggggggg cgtaaacgcc gcgtggatcc
     2221 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2281 atatatactg atatgtcggc ccataatagt aattctagct ggtttgatga attaaatatc
     2341 aatgataaaa tactatagta aaaataagaa taaataaatt aaaataatat ttttttatga
     2401 ttaatagttt attatataat taaatatcta taccattact aaatatttta gtttaaaagt
     2461 taataaatat tttgttagaa attccaatct gcttgtaatt tatcaataaa caaaatatta
     2521 aataacaagc taaagtaaca aataatatca aactaataga aacagtaatc taatgtaaca
     2581 aaacataatc taatgctaat ataacaaagc gcaagatcta tcaattttat atagtattat
     2641 tttcaatcaa cattctcatt aatttctaaa taatacttgt agttttatta acttctaaat
     2701 ggattgacta ttaattaaat gaattagtcg aacatgaata aacaaggtaa catgatagat
     2761 catgtcattg tgttatcatt gatcttacat ttggattgat tacagttggt ctagagattt
     2821 cgtctagatc gtatagccag gaatccaaat caagtcatct tccactttca actctccagg
     2881 aggaagagaa ataacaaacg agaaatcaaa cagtttggga acgcaatgat ggtccaagat
     2941 aatgttatgg gggttcaaga ttctgtagat aatgggagtt ggaaatgcag tgtgtagata
     3001 gagaattgca ctggcaatgc ttttggcaat cttcaatcta catttccaac ttaacaattt
     3061 atcgcaattg gggtcaagga aattagcaag agtctcaaat ctggcatctt catacactag
     3121 tgcctccgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     3181 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     3241 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     3301 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     3361 aattatcgcg cgcggtgtca tctatgttac tagatcggga attcactggc cgtcgtttta
     3421 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3481 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3541 cgcagcctga atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3661 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     3721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3781 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     3841 tttgccgatt tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     3901 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     3961 tcactggtga aaagaaaaac caccccagta cattaaaaac gtccgcaatg tgttattaag
     4021 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     4081 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     4141 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     4201 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     4261 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aaatatcatc tccctcgcag
     4321 agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct
     4381 tgagaactat gccgacataa taggaaatcg ctggataaag ccgctgagga agctgagtgg
     4441 cgctatttct ttagaagtga acgttgacga tatcaactcc cctatccatt gctcaccgaa
     4501 tggtacaggt cggggacccg aagttccgac tgtcggcctg atgcatcccc ggctgatcga
     4561 ccccagatct ggggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga
     4621 tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc
     4681 cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa
     4741 cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag
     4801 gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg
     4861 ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg
     4921 cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg
     4981 cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg
     5041 gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga
     5101 ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca
     5161 ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg
     5221 gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt
     5281 tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc
     5341 gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga
     5401 acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg
     5461 ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta
     5521 tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg
     5581 agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg
     5641 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     5701 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     5761 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     5821 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     5881 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     5941 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     6001 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     6061 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     6121 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     6181 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     6241 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     6301 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     6361 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     6421 ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag
     6481 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     6541 cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga
     6601 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
     6661 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
     6721 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
     6781 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
     6841 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
     6901 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
     6961 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
     7021 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
     7081 ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta
     7141 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     7201 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     7261 cgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     7321 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     7381 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
     7441 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     7501 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     7561 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     7621 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     7681 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     7741 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     7801 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     7861 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     7921 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     7981 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     8041 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct
     8101 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
     8161 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
     8221 cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta
     8281 caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg
     8341 atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa
     8401 aaactcaggc cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc
     8461 gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt
     8521 tacgcgtatg acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg
     8581 gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg
     8641 gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga
     8701 tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
     8761 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
     8821 ctgaacataa aacactatca ataagttgga gtcattaccc aattatgata gaatttacaa
     8881 gctataaggt tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc
     8941 cattctatag atatattgat aagcgcgctg cctatgcctt gccccctgaa atccttacat
     9001 acggcgatat cttctatata aaagatatat tatcttatca gtattgtcaa tatattcaag
     9061 gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg tagcttttta aatatggcgc
     9121 ttcatagagt aattctgtaa aggtccaatt ctcgttttca tacctcggta taatcttacc
     9181 tatcacctca aatggttcgc tgggtttatc gcacccccga acacgagcac ggcacccgcg
     9241 accactatgc caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat
     9301 gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc
     9361 acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg
     9421 ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc
     9481 gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg
     9541 aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg
     9601 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa
     9661 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct
     9721 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc
     9781 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa
     9841 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc
     9901 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa
     9961 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac
    10021 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg
    10081 gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac
    10141 ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctcggtct
    10201 tgccttgctc gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc
    10261 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
    10321 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
    10381 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
    10441 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
    10501 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
    10561 gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
    10621 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt
    10681 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
    10741 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
    10801 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
    10861 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
    10921 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg ccacgcttcc
    10981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11161 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11221 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    11281 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    11341 ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat
    11401 gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt
    11461 tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg
    11521 tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
    11581 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
    11641 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg
    11701 gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc
    11761 atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc
    11821 gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca
    11881 tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc
    11941 cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc
    12001 agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc
    12061 cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg
    12121 tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca
    12181 ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg
    12241 accagcggag gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc
    12301 tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc
    12361 tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc
    12421 tgtatgcgtt ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg
    12481 ggcggcggcc aatcttgctc gtctcgctgg ccggcgccag atctggggaa ccctgtggtt
    12541 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
    12601 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
    12661 atagtttaaa ctgaaggcgg gaaacgacaa tctgatcatg agcggagaat taagggagtc
    12721 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
    12781 cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc taagcacata
    12841 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
    12901 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
    12961 ctcatattca ctctcaatcc aaataatctg caccggatct ggatcgtttc gcatgattga
    13021 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    13081 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    13141 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
    13201 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    13261 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    13321 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    13381 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    13441 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
    13501 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgatga
    13561 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    13621 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    13681 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    13741 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    13801 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    13861 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    13921 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacggg
    13981 atctctgcgg aacaggcggt cgaaggtgcc gatatcatta cgacagcaac ggccgacaag
    14041 cacaacgcca cgatcctgag cgacaatatg atcgggcccg gcgtccacat caacggcgtc
    14101 ggcggcgact gcccaggcaa gaccgagatg caccgcgata tcttgctgcg ttcggatatt
    14161 ttcgtggagt tcccgccaca gacccggatg atccccgatc gttcaaacat ttggcaataa
    14221 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
    14281 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
    14341 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
    14401 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggcctcctgt
    14461 caatgctggc ggcggctctg gtggtggttc tggtggcggc tctgagggtg gtggctctga
    14521 gggtggcggt tctgagggtg gcggctctga gggaggcggt tccggtggtg gctctggttc
    14581 cggtgatttt gattatgaaa agatggcaaa cgctaataag ggggctatga ccgaaaatgc
    14641 cgatgaaaac gcgctacagt ctgacgctaa aggcaaactt gattctgtcg ctactgatta
    14701 cggtgctgct atcgatggtt tcattggtga cgtttccggc cttgctaatg gtaatggtgc
    14761 tactggtgat tttgctggct ctaattccca aatggctcaa gtcggtgacg gtgataattc
    14821 acctttaatg aataatttcc gtcaatattt accttccctc cctcaatcgg ttgaatgtcg
    14881 cccttttgtc tttggcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    14941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    15001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    15061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    15121 cgcc
//
