LOCUS       sequence-353498-       10078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterodimerization with iLID, RhoA activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Mahlandt EK, Palacios Martinez S, Arts JJG, Tol S, van Buul JD, 
            Goedhart J
  TITLE     Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control
            Rho GTPase activity on a global to subcellular scale, enabling 
            precise control over vascular endothelial barrier strength.
  JOURNAL   Elife. 2023 Jul 14;12:RP84364. doi: 10.7554/eLife.84364.
  PUBMED    37449837
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-14"; volume: "12:RP84364. doi"; pages: " 
            10.7554/eLife.84364"
COMMENT     Sequence Label: pLV-SspB-HaloTag-p63DH
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             549..878
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     regulatory      894..903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1902..1911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2596..3148
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2740..2757)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2967..2986
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3175..3774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3175..3194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3671..3691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4666..4899
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4971..5092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5008..5027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5062..5081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5132..5267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5287..5306)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5288..5306)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5316..5333)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5316..5332)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5325..5347)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5474..5929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5561..5580)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5771..5792
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5955..6059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6060..6128,6129..6920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6278..6297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7091..7679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7580..7599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7833..7850
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7967..7988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8003..8008,8009..8026,8027..8033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8041..8057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8046..8068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8065..8081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8065..8081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8100..8120
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8102..8120
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8148..8374
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8375..8555
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8602..8727
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9220..9453
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9638..9682
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9944..10061
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      541 ccaccatgag ctccccgaaa cgccctaagc tgctgcgtga atattacgat tggctggttg
      601 ataacagctt taccccatat ctggtggtgg atgccacata cctgggcgtg aacgtgcccg
      661 tggagtatgt gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc
      721 tgcaactgac aaatgatttt atccagttca acgcccgctt taagggcgtg tctcgtgaac
      781 tgtatatccc gatgggtgcc gctctggcca tttacgctcg cgagaacggc gatggtgtga
      841 tgttcgaacc agaagaaatc tatgacgagc tgaatattgg tagtggaccg gtcgccacca
      901 tggccgagat cggcaccggc ttccccttcg acccccacta cgtggaggtg ctgggcgagc
      961 gcatgcacta cgttgacgtg ggcccccgcg acggcacccc cgtgctgttc ctgcacggca
     1021 accccaccag cagctacgtg tggcgcaaca tcatccccca cgtggccccc acccaccgca
     1081 ctatcgcccc cgacctgatc ggcatgggca agagcgacaa gcccgacctg ggctacttct
     1141 tcgacgacca cgtgcgcttc atggacgcct tcatcgaggc cctgggcctg gaggaggtgg
     1201 tgctggtgat ccacgactgg ggcagcgccc tgggcttcca ctgggccaag cgcaaccccg
     1261 agcgcgtgaa gggcatcgcc ttcatggagt tcatccgccc catccccacc tgggacgagt
     1321 ggcccgagtt cgcccgcgag accttccagg ccttccgcac caccgacgtg ggccgcaagc
     1381 tgatcatcga ccagaacgtg ttcatcgagg gcaccctgcc catgggcgtg gtgcgccccc
     1441 tgaccgaggt ggagatggac cactaccgcg agcccttcct gaaccccgtg gaccgcgagc
     1501 ccctgtggcg cttccccaac gagctgccca tcgccggcga gcccgccaac atcgtggccc
     1561 tggtggagga gtacatggac tggctgcacc agagccccgt gcccaagctg ctgttctggg
     1621 gcacccccgg cgtgctgatc ccccccgccg aggccgcccg cctggccaag agcctgccca
     1681 acgttaaggc cgtggacatc ggccccggcc tgaacctgct ccaggaggac aaccccgacc
     1741 tgatcggcag cgagatcgcc cgctggctga gcaccctgga gatcagcggc gtgtacaagt
     1801 ccaagaaggc tctggaaagg agtatgtatg tcctgagtga actggtagaa acagagaaaa
     1861 tgtacgtgga cgacttgggg cagattgtgg agggttatat ggccaccatg gctgctcagg
     1921 gggtccccga gagtcttcga ggccgtgaca ggattgtgtt tgggaatatc cagcaaatct
     1981 atgagtggca ccgagactat ttcttgcaag agctacaacg gtgtctgaaa gatcctgatt
     2041 ggctggctca gctattcatc aaacacgagc gccggctgca tatgtatgtg gtgtactgtc
     2101 agaataagcc caagtcagag catgtggtgt cagagtttgg ggacagctac tttgaggagc
     2161 tccggcagca gctggggcac cgcctgcagc tgaacgacct cctcatcaaa cctgtgcagc
     2221 ggatcatgaa ataccagctg ctgctcaagg attttctcaa gtattacaat agagctggga
     2281 tggatactgc agacctagag caagctgtgg aggtcatgtg ctttgtgccc aagcgctgca
     2341 acgatatgat gacgctgggg agattgcggg gatttgaggg ctaaggtacc gcgggcccgg
     2401 gatccaccgg atctagccta gtgccatttg ttcagtggtt cgtagggctt tcccccactg
     2461 tttggctttc agttatatgg atgatgtggt attgggggcc aagtctgtac agcatcttga
     2521 gtcccttttt accgctgtta ccaattttct tttgtctttg ggtaaacccg ggaattccgc
     2581 ccctctcccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2641 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2701 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2761 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2821 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2881 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     2941 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3001 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3061 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3121 ttttcctttg aaaaacacga tgataatatg gatatggcca caacggtcgc caccatgacc
     3181 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     3241 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3301 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3361 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3421 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3481 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3541 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3601 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3661 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3721 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     3781 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatg tattcaatct
     3841 aagcaggctt tcactttctc gccaacttac aaggcctttc tgtgtaaaca atacctgaac
     3901 ctttaccccg ttgcccggca acggccaggt ctgtgccaag tgtttgctga cgcaaccccc
     3961 actggctggg gcttggtcat gggccatcag cgcatgcgtg gaaccttttc ggctcctctg
     4021 ccgatccata ctgcggaact cctagccgct tgttttgctc gcagcaggtc tggagcaaac
     4081 attatcggga ctgataactc tgttgtccta tcccgcaaat atacatcgtt tccatggctg
     4141 ctaggctgtg ctgccaactg gatcctgcgc gggacgtcct ttgtttacgt cccgtcggcg
     4201 ctgaatcctg cggacgaccc ttctcggggt cgcttgggac tctctcgtcc ccttctccgt
     4261 ctgccgttcc gaccgaccac ggggcgcacc tctctttacg cggactcccc gtctgtgcct
     4321 tctcatctgc cggaccgtgt gcacttcgct tcacctctgc acgtcgcatg gagaccaccg
     4381 tgaacgccca ccaaatattg cccaaggtct tacataagag gactcttgga ctctcagcaa
     4441 tgtcaacgac cgaccttgag gcatacttca aagactgttt gtttaaagac tgggaggagt
     4501 tgggggagga gattaggtta aaggtctttg tactaggagg ctgtaggcat aaattggtct
     4561 gcgcaccagc accatggcgc aatcactaga gcggggtacc tttaagacca atgacttaca
     4621 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4681 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4741 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4801 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4861 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4921 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4981 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5041 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5101 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5161 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5221 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5281 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5341 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5401 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5461 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5521 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5581 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5641 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5701 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5761 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5821 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5881 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5941 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6001 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6301 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6601 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6901 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6961 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7021 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7081 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7141 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7201 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7261 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7321 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7381 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7441 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7501 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7561 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7621 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7681 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7741 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7801 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7861 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7921 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7981 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8041 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8101 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     8161 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8221 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8281 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8341 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8401 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8461 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8521 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8581 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8641 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8701 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8761 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8821 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8881 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8941 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9001 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9061 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9121 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9181 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9241 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9301 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9361 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9421 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9481 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9541 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9601 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9661 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9721 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9781 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9841 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     9901 agacagatcc attcgattag tgaacggatc tcgacggtat cggttttaaa agaaaagggg
     9961 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10021 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgataag cttgggag
//
