LOCUS       sequence-353509-       11926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0346
FEATURES             Location/Qualifiers
     source          1..11926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4864..5452
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4917..4937)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5335..5346)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5524..5757
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5835..5956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5872..5891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5926..5945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5996..6131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6151..6170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6152..6170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6180..6197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6180..6196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6189..6211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6338..6793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6635..6656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6819..6923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6924..6992,6993..7784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7142..7161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7955..8543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8444..8463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8697..8714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8831..8852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8867..8872,8873..8890,8891..8897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8905..8921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8910..8932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8929..8945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8929..8945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8964..8984
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8966..8984
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9012..9238
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9239..9419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9466..9591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10084..10317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10502..10546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10777..10987
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10799..10995
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10846..10981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11027..11629
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11027..11046)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11523..11543
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11672..11789
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 ggtggacacc gaggagaagc ggcatcgcac gcggtccaaa ggggttcgag ttcccgtgga
     1261 accagccata caagagctgt tcagctgtcc cacccctggc tgtgacggca gtggtcatgt
     1321 cagtggcaaa tatgcaagac acagaagtgt atatggttgt cccttggcga aaaaaagaaa
     1381 aacacaagat aaacagcccc aggaacctgc tcctaaacga aagccatttg ccgtgaaagc
     1441 agacagctcc tcagtggatg agtgtgacga cagtgatggg actgaggaca tggatgagaa
     1501 ggaggaggat gagggggagg agtactccga ggacaatgat gagccagggg atgaggacga
     1561 ggaggacgag gagggggacc gggaggagga ggaggagatc gaggaggagg atgaggacga
     1621 tgacgaggat ggagaagatg tggaggatga agaagaggaa gaggaggagg aggaggagga
     1681 ggaagaggaa gaagaaaacg aagaccatca aatgaattgt cacaatactc gaataatgca
     1741 agacacagaa aaggatgata acaataatga cgaatatgac aattacgatg aactggtggc
     1801 caagtcattg ttaaacctcg gcaaaatcgc tgaggatgca gcctaccggg ccaggactga
     1861 gtcagaaatg aacagcaata cctccaatag tctggaagac gatagtgaca aaaacgaaaa
     1921 cctgggtcgg aaaagtgagt tgagtttaga cttagacagt gatgttgtta gagaaacagt
     1981 ggactccctt aaactattag cccaaggaca cggtgttgtg ctctcagaaa acatgaatga
     2041 cagaaattat gcagacagca tgtcgcagca agacagtaga aatatgaatt acgtcatgtt
     2101 ggggaagccc atgaacaacg gactcatgga aaagatggtg gaggagagcg atgaggaggt
     2161 gtgtctgagc agtctggagt gtttgaggaa tcagtgcttc gacctggcca ggaagctcag
     2221 tgagaccaac ccgcaggaga ggaatccgca gcagaacatg aacatccgtc agcatgtccg
     2281 gccagaagag gacttccccg gaaggacgcc ggacagaaac tactcggaca tgctgaacct
     2341 catgcggctg gaggagcagt tgagcccccg gtcgagagtg tttgccagct gtgcgaagga
     2401 ggatgggtgt catgagcggg acgacgatac cacctctgtg aactcggaca ggtctgaaga
     2461 ggtgttcgac atgaccaagg ggaacctgac cctgctggag aaagccatcg ctttggaaac
     2521 ggaaagagca aaggccatga gggagaagat ggccatggaa gctgggagga gggacaatat
     2581 gaggtcatat gaggaccagt ctccgagaca acttcccggg gaggacagaa agcctaaatc
     2641 cagtgacagc catgtcaaaa agccatacta tggtaaagat ccctcaagaa cagaaaagaa
     2701 agagagcaag tgtccaaccc ccgggtgtga tggaaccggc cacgtaactg ggctgtaccc
     2761 acatcaccgc agcctgtccg gatgcccgca caaagatagg gtccctccag aaatccttgc
     2821 catgcatgaa agtgtcctca agtgccccac tccgggctgc acggggcgcg ggcatgtcaa
     2881 cagcaacagg aactcccacc gaagcctctc cggatgcccg atcgctgcag cagagaaact
     2941 ggccaaggca caggaaaagc accagagctg cgacgtgtcc aagtccagcc aggcctcgga
     3001 ccgcgtgctc aggccaatgt gctttgtgaa gcagctggag attcctcagt atggctacag
     3061 aaacaatgtc cccacaacta cgccgcgttc caacctggcc aaggagctcg agaaatattc
     3121 caagacctcg tttgaataca acagttacga caaccatact tatggcaagc gagccatagc
     3181 tcccaaggtg caaaccaggg atatatcccc caaaggatat gatgatgcga agcggtactg
     3241 caaggacccc agccccagca gcagcagcac cagcagctac gcgcccagca gcagcagcaa
     3301 cctgagctgc ggcgggggca gcagcgccag cagcacgtgc agcaagagca gcttcgacta
     3361 cacgcacgac atggaggcgg cccacatggc ggccaccgcc atcctcaacc tgtccacgcg
     3421 ctgccgcgag atgccgcaga acctgagcac caagccgcag gacctgtgcg ccacgcggaa
     3481 ccctgacatg gaggtggatg agaacgggac cctggacctc agcatgaaca agcagaggcc
     3541 gcgggacagc tgctgcccca tcctgacccc tctggagccc atgtcccccc agcagcaggc
     3601 agtgatgaac aaccggtgtt tccagctggg cgagggcgac tgctgggact tgcccgtaga
     3661 ctacaccaaa atgaaacccc ggaggataga cgaggacgag tccaaagaca ttactccaga
     3721 agacttggac ccattccagg aggctctaga agaaagacgg tatcccgggg aggtgaccat
     3781 cccaagtccc aaacccaagt accctcagtg caaggagagc aaaaaggact taataactct
     3841 gtctggctgc cccctggcgg acaaaagcat tcgaagtatg ctggccacca gctcccaaga
     3901 actcaagtgc cccacgcctg gctgtgatgg ttctggacat atcaccggca attatgcttc
     3961 tcatcggagc ctttcaggtt gcccaagagc aaagaaaagt ggtatcagga tagcacagag
     4021 caaagaagat aaagaagatc aagaacccat caggtgtccg gtccccgggt gcgacggcca
     4081 gggccacatc actgggaagt acgcgtccca tcgcagcgcc tccgggtgcc ccttggcggc
     4141 caagaggcag aaagacgggt acctgaatgg ctcccagttc tcctggaagt cggtcaagac
     4201 ggaaggcatg tcctgcccca cgccaggatg cgacggctca ggccacgtca gcggcagctt
     4261 cctcacacac cgcagcttgt caggatgccc gagagccacg tcagcgatga agaaggcaaa
     4321 gctttctgga gagcagatgc tgaccatcaa acagcgggcc agcaacggta tagaaaatga
     4381 tgaagaaatc aaacagttag atgaagaaat caaggagcta aatgaatcca attcccagat
     4441 ggaagccgat atgattaaac tcagaactca gattaccacg atggagagca acctgaagac
     4501 catcgaagag gagaacaaag tgattgagca gcagaacgag tctctcctcc acgagctggc
     4561 gaacctgagc cagtctctga tccacagcct ggctaacatc cagctgccgc acatggatcc
     4621 aatcaatgaa caaaattttg atgcttacgt gactactttg acggaaatgt atacaaatca
     4681 agatcgttat cagagtccag aaaataaagc cctactggaa aatataaagc aggctgtgag
     4741 aggaattcag gtctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     4801 ttatatatct tgtggaaagg acgaagatac tgtagtgaac aacgtcgaac gcgttaagtc
     4861 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4921 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4981 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5041 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5101 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5161 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5221 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5281 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5341 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5401 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     5461 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5521 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5581 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5641 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5701 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5761 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5821 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5881 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5941 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6001 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6061 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6121 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6301 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6781 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7021 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7081 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7141 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7201 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7261 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7321 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7441 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8941 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9001 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     9061 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     9121 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     9181 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     9241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9421 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9481 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9541 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9601 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9661 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9721 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9781 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9841 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9901 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9961 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10021 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10081 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10141 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10201 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10261 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10321 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10381 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10441 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10501 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10561 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10621 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10681 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
    10741 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10801 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10981 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
    11041 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    11101 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
    11161 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    11221 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
    11281 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    11341 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    11401 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    11461 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    11521 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    11581 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
    11641 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    11701 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    11761 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    11821 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
    11881 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
