LOCUS       sequence-353510-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0337
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4459..5047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4512..4532)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4930..4941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5119..5352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5430..5551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5467..5486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5521..5540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5591..5726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5746..5765)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5747..5765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5775..5792)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5775..5791)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5784..5806)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5933..6388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6020..6039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6230..6251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6414..6518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6519..6587,6588..7379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6737..6756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7550..8138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8039..8058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8292..8309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8426..8447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8462..8467,8468..8485,8486..8492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8500..8516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8505..8527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8524..8540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8524..8540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8559..8579
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8561..8579
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8607..8833
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8834..9014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9061..9186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9679..9912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10097..10141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10372..10582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10394..10590
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10441..10576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10622..11224
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10622..10641)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11118..11138
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11267..11384
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 gagcgaagac tatccccatg aaactatggc gccggatatc cacgaagaac ggcaatatcg
     1261 ctgcgaagac tgtgaccagc tctttgaatc taaggctgaa ctagcagatc accaaaagtt
     1321 tccatgcagt actcctcact cagcattttc aatggttgaa gaggactttc agcaaaaact
     1381 cgaaagcgag aatgatctcc aagagataca cacgatccag gagtgtaagg aatgtgacca
     1441 agtttttcct gatttgcaaa gcctggagaa acacatgctg tcacatactg aagagaggga
     1501 atacaagtgt gatcagtgtc ccaaggcatt taactggaag tccaatttaa ttcgccacca
     1561 gatgtcacat gacagtggaa agcactatga atgtgaaaac tgtgccaagg ttttcacgga
     1621 ccctagcaac cttcagcggc acattcgctc tcagcatgtc ggtgcccggg cccatgcatg
     1681 cccggagtgt ggcaaaacgt ttgccacttc gtcgggcctc aaacaacaca agcacatcca
     1741 cagcagtgtg aagcccttta tctgtgaggt ctgccataaa tcctatactc agttttcaaa
     1801 cctttgccgt cataagcgca tgcatgctga ttgcagaacc caaatcaagt gcaaagactg
     1861 tggacaaatg ttcagcacta cgtcttcctt aaataaacac aggaggtttt gtgagggcaa
     1921 gaaccatttt gcggcaggtg gattttttgg ccaaggcatt tcacttcctg gaaccccagc
     1981 tatggataaa acgtccatgg ttaatatgag tcatgccaac ccgggccttg ctgactattt
     2041 tggcgccaat aggcatcctg ctggtcttac ctttccaaca gctcctggat tttcttttag
     2101 cttccctggt ctgtttcctt ccggcttgta ccacaggcct cctttgatac ctgctagttc
     2161 tcctgttaaa ggactatcaa gtactgaaca gacaaacaaa agtcaaagtc ccctcatgac
     2221 acatcctcag atactgccag ctacacagga tattttgaag gcactatcta aacacccatc
     2281 tgtaggggac aataagccag tggagctcca gcccgagagg tcctctgaag agaggccctt
     2341 tgagaaaatc agtgaccagt cagagagtag tgaccttgat gatgtcagta caccaagtgg
     2401 cagtgacctg gaaacaacct cgggctctga tctggaaagt gacattgaaa gtgataaaga
     2461 gaaatttaaa gaaaatggta aaatgttcaa agacaaagta agccctcttc agaatctggc
     2521 ttcaataaat aataagaaag aatacagcaa tcattccatt ttctcaccat ctttagagga
     2581 gcagactgcg gtgtcaggag ctgtgaatga ttctataaag gctattgctt ctattgctga
     2641 aaaatacttt ggttcaacag gactggtggg gctgcaagac aaaaaagttg gagctttacc
     2701 ttacccttcc atgtttcccc tcccattttt tccagcattc tctcaatcaa tgtacccatt
     2761 tcctgataga gacttgagat cgttaccttt gaaaatggaa ccccaatcac caggtgaagt
     2821 aaagaaactg cagaagggca gctctgagtc cccctttgat ctcaccacta agcgaaagga
     2881 tgagaagccc ttgactccag tcccctccaa gcctccagtg acacctgcca caagccaaga
     2941 ccagcccctg gatctaagta tgggcagtag gagtagagcc agtgggacaa agctgactga
     3001 gcctcgaaaa aaccacgtgt ttgggggaaa aaaaggaagc aacgtcgaat caagacctgc
     3061 ttcagatggt tccttgcagc atgcaagacc cactcctttc tttatggacc ctatttacag
     3121 agtagagaaa agaaaactaa ctgacccact tgaagcttta aaagagaaat acttgaggcc
     3181 ttctccagga ttcttgtttc acccacaatt ccaactgcct gatcagagaa cttggatgtc
     3241 agctattgaa aacatggcag aaaagctaga gagcttcagt gccctgaaac ctgaggccag
     3301 tgagctctta cagtcagtgc cctctatgtt caacttcagg gcgcctccca atgccctgcc
     3361 agagaacctt ctgcggaagg gaaaggagcg ctatacctgc agatactgtg gcaagatttt
     3421 tccaaggtct gcaaacctaa cacggcactt gagaacccac acaggagagc agccttacag
     3481 atgcaaatac tgtgacagat catttagcat atcttctaac ttgcaaaggc atgttcgcaa
     3541 catccacaat aaagagaagc catttaagtg tcacttatgt gataggtgtt ttggtcaaca
     3601 aaccaattta gacagacacc taaagaaaca tgagaatggg aacatgtccg gtacagcaac
     3661 atcgtcgcct cattctgaac tggaaagtac aggtgcgatt ctggatgaca aagaagatgc
     3721 ttacttcaca gaaattcgaa atttcattgg gaacagcaac catggcagcc aatctcccag
     3781 gaatgtggag gagagaatga atggcagtca ttttaaagat gaaaaggctt tggtgaccag
     3841 tcaaaattca gacttgctgg atgatgaaga agttgaagat gaggtgttgt tagatgagga
     3901 ggatgaagac aatgatatta ctggaaaaac aggaaaggaa ccagtgacaa gtaatttaca
     3961 tgaaggaaac cctgaggatg actatgaaga aaccagtgcc ctggagatga gttgcaagac
     4021 atccccagtg aggtataaag aggaagaata taaaagtgga ctttctgctc tagatcatat
     4081 aaggcacttc acagatagcc tcaaaatgag gaaaatggaa gataatcaat attctgaagc
     4141 tgagctgtct tcttttagta cttcccatgt gccagaggaa cttaagcagc cgttacacag
     4201 aaagtccaaa tcgcaggcat atgctatgat gctgtcactg tctgacaagg agtccctcca
     4261 ttctacatcc cacagttctt ccaacgtgtg gcacagtatg gccagggctg cggcggaatc
     4321 cagtgctatc cagtccataa gccacgtata gtaatgaact agtccgtaac ttgaaagtat
     4381 ttcgatttct tggctttata tatcttgtgg aaaggacgat aatacccatg cggtacaccc
     4441 tgcacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4501 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4561 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4621 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4681 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4741 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4801 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4861 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4921 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4981 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5041 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5101 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5161 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5221 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5281 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5341 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     5401 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5581 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5641 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5701 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5761 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5821 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5881 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6361 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6421 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6481 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6541 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6601 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6661 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6721 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6781 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6841 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6901 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6961 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7021 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7081 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7141 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7201 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7261 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7321 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7381 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7441 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7681 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7921 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8101 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8161 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8221 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8281 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8341 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8401 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8461 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8521 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8581 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     8641 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     8701 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     8761 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8821 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8881 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8941 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9001 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9061 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9121 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9181 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9241 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     9301 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     9361 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     9421 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     9481 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     9541 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9601 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9661 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9721 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9781 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9841 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9901 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9961 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10021 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10081 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10141 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10201 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10261 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
    10321 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10381 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
    10441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10561 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
    10621 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    10681 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    10741 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10801 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    10861 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10921 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10981 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    11041 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    11101 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    11161 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    11221 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    11281 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    11341 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    11401 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    11461 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    11521 t
//
