LOCUS       sequence-353511-       11305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2437
FEATURES             Location/Qualifiers
     source          1..11305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4243..4831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4296..4316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4714..4725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4903..5136
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5214..5335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5251..5270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5305..5324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5375..5510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5530..5549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5531..5549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5559..5576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5559..5575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5568..5590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5717..6172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5804..5823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6014..6035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6198..6302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6303..6371,6372..7163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6521..6540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7334..7922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7823..7842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8076..8093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8210..8231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8246..8251,8252..8269,8270..8276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8284..8300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8289..8311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8308..8324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8308..8324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8343..8363
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8345..8363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8391..8617
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8618..8798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8845..8970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9463..9696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9881..9925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10156..10366
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10178..10374
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10225..10360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10406..11008
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10406..10425)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10902..10922
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11051..11168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 cgacaaccct tttcaaccaa aaagtaattc aaaaatggca gaactgttta tggaatgtga
     1261 agaagaggag ctggaaccat ggcagaagaa agtaaaagaa gttgaggatg acgatgatga
     1321 tgagccaatc tttgttggcg agatatcaag ttcaaaacca gcaatttcaa atattttgaa
     1381 cagagttaac cccagctcat attcaagggg actaaagaat ggtgcactca gtcgaggtat
     1441 tactgctgca ttcaagccta caagtcaaca ctacacgaat ccaacatcaa atccagtgcc
     1501 tgcctcacca ataaattttc atcctgagtc tagatcttca gatagttctg ttattgttca
     1561 gcctttttct aaacctggtt atataacaaa ctcatcacga gttgtgtcta ataagtcatc
     1621 agagttactg tttgacttga cccaggatac aggattatca cattaccaag ggggaccaac
     1681 actttctatg gcaggtatga gtgaaagttc atttttatca aaacgtcctt ctacttctga
     1741 agtaaataat gttaatccaa agaagcctaa acccagcgaa agtgtttctg gagcaaattc
     1801 ctcagctgta ttaccttcag ttaaatctcc ttcagtgact tcttcccagg ctatgctagc
     1861 aaaaggtaca aatacctcat caaatcagtc taaaaatgga acaccttttc caagagcttg
     1921 tccaaagtgc aacattcatt tcaatctttt ggatcctttg aaaaatcaca tgaagtattg
     1981 ttgtccagac atgataaata actttttggg actggctaaa acagaatttt caagtacagt
     2041 aaataaaaac acaactattg attcagagaa aggaaaattg atcatgttag ttaatgactt
     2101 ttattatgga aaacatgaag gagatgtcca ggaggaacag aagactcaca caacctttaa
     2161 atgcttcagt tgcttgaaaa ttctaaaaaa taacattagg tttatgaacc acatgaaaca
     2221 ccatttggaa cttgagaagc agagcagtga gagctgggaa aaccatacca cctgccagca
     2281 ctgctaccgt cagtttccca caccatttca gttgcagtgt cacatcgaaa gtacacatac
     2341 gccccatgaa ttttctacta tttgtaaaat ctgtgaattg tcatttgaaa cagaacatgt
     2401 tcttttacaa catatgaagg acaatcataa acctggtgaa atgccatatg tctgccaggt
     2461 ttgtaattat agatcatcat cattttctga tgtagaaact cattttagaa catcccatga
     2521 aaacactaag aacttgctat gcccgttttg cctcaaagtt attaaaattg caacaccata
     2581 tatgcatcat tatatgaagc atcagaaaaa aggaatacat cgttgtacaa aatgcaggct
     2641 gcagtttttg acatgcaagg agaaaatgga tcataagact caacaccatc gaacatttat
     2701 aaaacctaaa caactagaag gattgcctcc tggaacaaaa gttactattc gagcttcagt
     2761 tggacctctg caatcaggag cttcacctac accttccatt agtgcaagtg cttctaccct
     2821 tcagctctca cctccaagga ctaaaaatat aactgctaaa aatcctgcaa aatctaatac
     2881 aagtaaacct aatacagtca aatccaacgc aagtaaacct aatacaagta agcctaatgg
     2941 aagtaaatct aaatacaaac caaaaatctc taatatgcaa aagaaacaaa gcacattggc
     3001 tagtagcaat aaaaaaagta aagtcaatac agcattgagg aatttaaggt atcgtcgggg
     3061 cattcacaaa tgcattgagt gttgttccga aataaaagat tttgcaaacc actttcctac
     3121 gtacgtccac tgtagttttt gcagatacaa cactagctgt agcaaagcct atgtaaatca
     3181 tatgatgagc tttcatagta accgtccaag caaaaggttt tgtattttta agaagcattc
     3241 agaaaatctc cggggcatta ctctagtgtg ccttaattgt gatttcctaa gtgatgtttc
     3301 tggcttagat aatatggcta cacacttaag tcaacataaa actcatactt gccaagttgt
     3361 aatgcagaaa gtttctgttt gtatcccaac ttctgagcac ctttctgaat taaaaaaaga
     3421 agctcccgca aaggaacaag aacctgtgtc taaggaaatt gcaagaccta acatggctga
     3481 aagagaaaca gaaacatcaa attctgaaag taaacaagat aaagctgctt cttcaaaaga
     3541 aaaaaatgga tgtaatgcaa attcatttga aggctcatca acaacaaaaa gtgaagaaag
     3601 cataacagtt tcagataagg aaaatgaaac ctgtcttgca gaccaggaaa ctggctcaaa
     3661 aaacatcgtc agttgtgatt caaatattgg tgcagataaa gtggaaaaga aaaaacaaat
     3721 acaacacgtt tgtcaggaaa tggagttgaa gatgtgccaa agttcagaaa acataatctt
     3781 atctgatcag attaaagatc acaactccag tgaagccaga ttttcttcaa agaatattaa
     3841 ggatttgcga ttagcatcag ataatgtaag cattgatcag tttttgagaa aaagacatga
     3901 acctgaatct gttagttctg atgttagcga gcaaggcagt attcatttgg aacctctgac
     3961 tccatccgag gtacttgagt atgaagccac agagattctt cagaaaggta gtggtgatcc
     4021 ttcagccaag actgatgaag tagtgtctga tcaaacagat gacattcctg gaggaaataa
     4081 ccctagcaca acagaggcaa cagtagacct ggaagacgaa aaagaaagaa gttagtaatg
     4141 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4201 cgatcgtcag attcaaaggg cccggtaacg cgttaagtcg acaatcaacc tctggattac
     4261 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4321 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4381 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4441 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4501 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4561 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4621 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4681 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4741 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4801 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4861 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4921 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5101 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5341 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5521 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5641 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5701 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6121 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6541 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8101 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8161 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8221 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8281 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8341 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8401 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8461 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8521 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8581 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8641 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8701 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8761 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8821 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8881 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8941 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9001 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9061 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9121 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9181 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9241 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9301 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9361 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9421 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9481 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9541 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9601 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9661 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9721 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9781 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9841 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9901 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9961 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10021 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10081 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
    10141 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
    10201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10321 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    10381 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    10441 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    10501 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    10561 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    10621 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    10681 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    10741 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    10801 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    10861 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    10921 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    10981 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    11041 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11101 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11161 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    11221 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    11281 ctaggtcttg aaaggagtgg gaatt
//
