LOCUS       sequence-353515-       15103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1802
FEATURES             Location/Qualifiers
     source          1..15103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8041..8629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8094..8114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8512..8523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8701..8934
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9012..9133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9049..9068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9103..9122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9173..9308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9328..9347)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9329..9347)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9357..9374)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9357..9373)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9366..9388)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9515..9970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9602..9621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9812..9833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9996..10100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10101..10169,10170..10961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10319..10338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11132..11720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11621..11640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11874..11891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12008..12029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12044..12049,12050..12067,12068..12074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12082..12098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12087..12109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12106..12122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12106..12122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12141..12161
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12143..12161
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12189..12415
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12416..12596
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12643..12768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13261..13494
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13679..13723
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13954..14164
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13976..14172
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      14023..14158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14204..14806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(14204..14223)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14700..14720
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14849..14966
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 agaaatgaca gagaatgaga cgcctacaaa aaagcagcac agaaagaaaa accgggagac
     1261 acacaatgca gtggagaggc atagaaagaa gaaaattaat gctgggataa acagaatagg
     1321 agagctgatc ccatgttctc ctgccctgaa gcagagcaag aatatgatcc tggaccaagc
     1381 ctttaaatat ataacagaat tgaaaaggca aaatgatgaa ctcctgctta atggaggaaa
     1441 caatgaacaa gctgaagaaa taaaaaagct acggaaacaa ctggaagaaa tccaaaaaga
     1501 aaatggccga tatattgaat tactgaaagc taatgacata tgcttatatg atgacccgac
     1561 aattcactgg aaaggaaatc ttaaaaactc aaaggtctct gttgttattc ctagtgacca
     1621 ggttcaaaaa aaaattattg tttattccaa cgggaatcag cctggtggaa acagccaggg
     1681 aacagctgtt caggggataa cttttaatgt tagtcataat ttacaaaagc agaccgccaa
     1741 tgtggtgcca gtacagagga cttgcaatct tgtgactcct gtatctattt ctggagttta
     1801 cccttctgaa aacaagccat ggcatcagac cacagttcct gcattggcta ccaaccagcc
     1861 tgttcctctt tgtcttcctg ctgccatttc tgctcagagt attctcgagc ttcccacctc
     1921 tgaaagcgaa tcaaatgtgc ttggtgccac tagtggctca ctgattgctg tttcaattga
     1981 atctgagcct caccaacatc attctttgca cacatgccta aatgatcaaa attcttctga
     2041 aaataagaat ggacaagaga accccaaagt attgaagaaa atgacccctt gtgttacaaa
     2101 catcccccac agctcctcag caactgccac taaagtgcac catggaaaca agtcctgcct
     2161 gagcatacag gacttcagag gtgattttca aaacactttt gttgtttcag ttaccaccac
     2221 agtctgctcg cagcctccca gaactgcagg tgattcttct ccaatgagca ttagtaagag
     2281 tgcagacttg acaagtacag ctacagtggt ggcatcatct gcccctggag tagggaaggc
     2341 caccattcct ataagcactc tttcgggaaa ccctttggac aatggttgga ctctttcttg
     2401 ttctttgcct tcttcaagtg ttagtacttc agatttgaaa aacattaata gccttacacg
     2461 aatctcttca gctggaaaca cacagacaac gtggactact ttgcaactgg caggaaacac
     2521 tattcagccc ttaagccaga caccatcttc tgctgtgact ccagtattaa atgagtctgg
     2581 tactagcccc accacaagca accacagtag atatgtggct acagacatca acttgaataa
     2641 ttcctttcca gcagatgggc agccagttga gcaagtagtt gtaacattgc cttcttgtcc
     2701 atctttacct atgcagccac taattgccca gccacaagtt aaatctcagc ctcccaaaaa
     2761 tatccttcca ctgaattcag caatgcaagt gattcagatg gctcagccag ttgggtcagc
     2821 tgttaattca gctccaacta atcaaaatgt tataattctt cagccaccta gcaccacccc
     2881 atgcccaaca gtgatgaggg cagaagtttc caaccaaaca gtaggtcaac agatagtaat
     2941 catacaggct gctaatcaga atcctttgcc actcctccct gctccacctc ctggttctgt
     3001 tcgactcccc atcaatggag ccaatactgt aatagggtct aataattcag tgcaaaatgt
     3061 tccaacacca cagacttttg gaggaaagca tcttgtccac atattaccaa gaccttcatc
     3121 tttatcagcg tctaactcaa cacaaacttt ttctgttacc atgtcaaacc aacagcctca
     3181 aaccatttct ttaaatggac agctctttgc tttgcagcct gtgatgtctt catcaggaac
     3241 tacaaatcaa acccctatgc aaattattca acccaccacc agcgaagatc caaataccaa
     3301 tgttgccctg aatacatttg gtgctttggc cagcctcaat caaagcatat cacagatggc
     3361 tgggcaaagc tgtgtacaat tgtctattag ccagcctgcc aattctcaaa ctgctgcaaa
     3421 tagtcaaacc actacagcta actgtgtttc attaacaaca actgcagcac ctcctgtgac
     3481 aacagatagt tcagccacac tagctagtac ttataatcta gtgagtactt cctcaatgaa
     3541 cactgttgct tgtttgccta acatgaaatc taaaaggttg aataagaagc caggtggcag
     3601 gaaacactta gcagcaaaca agtcagcgtg tcccctgaat tcagtcagag atgtgagcaa
     3661 gttagactgc cccaacactg aaggctcagc agagccaccc tgtaatgatg gactgctaga
     3721 aagcttccct gctgtgttac catctgtctc tgtgtctcag gcaaatagtg tgagtgtttc
     3781 tgcttcacat tctttgggtg ttctaagctc tgaatcatta atacctgagt ctgtatcgaa
     3841 atctaagtca gcagaaaagt ccagcccacc ctcccaagaa tctgtaacaa gtgaacattt
     3901 tgcaatggcc gcagcaaaat ccaaagattc tacccctaat ctacaacaag agacatctca
     3961 ggataaacca ccaagtagtt tagcattatc agatgctgcc aaaccctgcg cttcagccaa
     4021 tgtattgatt ccatctccaa gtgatcctca cattttggtt tctcaggttc ctggtttgtc
     4081 atctacaaca agcactacaa gtactgactg tgtttctgag gtagaaatca ttgctgaacc
     4141 ttgcagagtt gagcaagatt catcagatac aatgcaaacc acaggtctct taaaggggca
     4201 aggtttaact acattgctat ctgatcttgc taaaaaaaaa aaccctcaga aatcatctct
     4261 ttctgatcag atggatcatc ctgacttttc ttcagaaaat cctaaaatag ttgattcaag
     4321 tgtgaattta catcccaaac aggaactatt actgatgaac aatgatgata gagatcctcc
     4381 acagcatcat tcctgcctcc ctgatcaaga ggttattaat ggttctttga tcaacggtag
     4441 acaggccgac tctcccatgt caaccagctc tggcagtagt cgtagtttct cagttgcatc
     4501 catgcttcct gaaacaacaa gagaagatgt gaccagcaat gcaacaacta atacatgtga
     4561 cagctgtacc tttgtagagc aaactgatat agtagctctt gcagcaagag ctatttttga
     4621 ccaggagaac cttgagaagg ggagagttgg cctccaggct gatataaggg aagttgcttc
     4681 aaagccttct gaagcatcat tgttagaggg agacccaccc ttcaaatcac agatacctaa
     4741 agagagtggc acaggacagg cagaagcaac accaaatgaa tttaattctc agggttcaat
     4801 tgaagcaact atggagaggc cccttgagaa accaagttgt tctctaggaa ttaaaacatc
     4861 aaatgcatct ttacaggatt caacttctca gccaccaagc atcaccagtt taagtgtgaa
     4921 taatcttatc catcagagca gcatcagcca tcctctggcc agctgtgcgg gtttatcccc
     4981 aacttcagag caaacaactg tgcctgcaac ggttaatctg accgtttcat ctagctccta
     5041 tggcagtcaa cctcctggac catctctgat gactgaatat tcccaagaac agctaaatac
     5101 tatgactagt accataccaa attcacagat tcaagagcca ctcttaaagc caagccatga
     5161 aagccgtaag gattctgcta agcgtgctgt ccaagatgac cttttactgt cttcagctaa
     5221 acgtcaaaag cactgtcagc cagcccccct caggcttgaa agtatgtccc tgatgagcag
     5281 aactccagac accatttctg accaaactca aatgatggtc agtcagatcc ctcctaattc
     5341 ttcaaactca gttgtgcctg ttagcaaccc agctcatgga gatggcctta cacgattatt
     5401 tccacctagt aacaactttg tgactcctgc attgaggcaa actgaagttc agtgtggttc
     5461 tcagccttca gttgctgaac agcagcaaac ccaggcaagt cagcatctac aggccctgca
     5521 gcagcatgtt ccagctcaag gtgtatctca ccttcatagt aaccatctct acataaagca
     5581 gcagcagcag cagcagcagc agcagcagca gcaacagcaa caacaacaag cagggcagtt
     5641 aagagagagg catcacttat atcaaatgca gcatcatgta cctcatgcag agagctctgt
     5701 ccactctcag ccccataatg tccaccaaca gaggactctg caacaggaag ttcagatgca
     5761 gaaaaagagg aatcttgttc agggcaccca gacctctcag ctttccttac aaccaaagca
     5821 ccatggaact gaccaatccc gatccaagac tggccagcca catccccacc atcagcagat
     5881 gcagcaacaa atgcagcaac actttggaag ctcccagaca gagaagagct gtgaaaaccc
     5941 ttcaactagt cggaaccatc ataaccatcc ccagaaccat ctcaatcaag atattatgca
     6001 ccaacagcag gatgttggaa gcagacagca aggttcaggg gtttcatctg aacatgtatc
     6061 tggccataat ccaatgcaga ggcttttgac atcaagaggc ttagagcagc aaatggtgtc
     6121 ccaaccaagt attgtgacta gatcttcaga catgacctgt actccacaca ggccagagag
     6181 aaatagagtt tcaagttatt ctgctgaggc actcattgga aagacatctt ctaattcaga
     6241 gcagagaatg gggatatcaa ttcagggttc cagagtttca gatcagcttg aaatgagaag
     6301 ctatcttgat gttcccagaa ataagagttt ggctattcat aatatgcagg gtcgtgtgga
     6361 ccatactgtg gcctcagata tccgcctttc tgattgtcag acgtttaaac caagtggagc
     6421 tagtcaacag ccccagagta attttgaagt acaatcttca agaaacaatg aaataggtaa
     6481 ccctgtatca tcattgcgga gtatgcagtc ccaggctttt cgaattagtc aaaacactgg
     6541 ccccccacca attgaccgtc aaaagagatt atcttaccca ccagttcaga gcatcccaac
     6601 aggaaatggt attccatcaa gggacagtga aaatacttgt caccaaagtt tcatgcagag
     6661 cttacttgcc cctcacctca gtgatcaggt cattgggagc cagaggtcac tctcagaaca
     6721 tcagaggaat acacagtgtg gtccatcctc tgcaattgaa tataattgtc ccccaactca
     6781 tgaaaatgtc catattagaa gagagagtga gagtcagaat agggaaagtt gtgacatgtc
     6841 gttaggtgca attaacacca ggaacagcac cttgaatatt cctttttcaa gttcctcttc
     6901 ctcaggagat attcaaggtc gaaacacaag ccccaatgtt tctgtacaga aatccaatcc
     6961 catgaggatt actgagagtc atgccaccaa gggccacatg aaccctccag tcacaaccaa
     7021 catgcatggg gttgcaaggc cagcgttgcc acatccatct gtgtctcatg gaaatggcga
     7081 tcaaggccct gctgtacgtc aagctaattc ttcagttccc cagagatcaa ggcatcccct
     7141 gcaagacagc agtggttcca aaattcgtca gcctgaaagg aatcgttctg gaaaccaaag
     7201 gcaaagtact gtctttgatc caagtcttcc ccatcttcct ctctctactg gtggcagtat
     7261 gattcttgga cgtcaacaac ctgccacaga gaagagagga agtattgttc gtttcatgcc
     7321 tgatagccca caagtaccta atgataattc aggtcctgac cagcatacac tatcacaaaa
     7381 ttttggtttt tcttttattc ctgagggtgg catgaatcca ccaataaatg ctaatgcttc
     7441 tttcattcca caggttactc agcctagtgc cactcgaact ccagccctca tcccggtaga
     7501 tccgcaaaat actctgccct ccttctatcc tccatactct cctgctcatc ctacactgtc
     7561 caatgatatt tcaatcccct attttcctaa tcagatgttc tcaaatccta gcacagagaa
     7621 ggtaaacagt ggaagtttaa ataaccgatt tggatcaatt ttatctcctc ccagacctgt
     7681 tgggtttgct caaccaagtt ttcctcttct cccagatatg ccaccaatgc acatgaccaa
     7741 ctctcactta tccaatttta atatgacatc tttgtttcca gaaatagcca cagcgcttcc
     7801 tgatggctca gcaatgtcac ctttgcttac aatagcaaat tcctctgcct ctgactcttc
     7861 caagcagtcc tcaaacagac ctgcccataa cataagccat attctaggtc atgattgcag
     7921 ttcagctgtt tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     7981 tatatcttgt ggaaaggacg acaaccttag acctaaggaa cggaaacgcg ttaagtcgac
     8041 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8101 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8161 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8221 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8281 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8341 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8401 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8461 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8521 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8581 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     8641 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8701 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8761 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8821 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8881 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     8941 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9001 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9121 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     9181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9241 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9301 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9361 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9421 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9481 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9541 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9601 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9661 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9721 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9781 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9841 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9901 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9961 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10021 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10081 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10141 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10201 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10261 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10321 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10381 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10441 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10501 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10561 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10621 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10681 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10741 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10801 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10861 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10921 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10981 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11041 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11521 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    12001 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    12061 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    12121 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    12181 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
    12241 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
    12301 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
    12361 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
    12421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    12481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    12541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    12601 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    12661 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    12721 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    12781 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    12841 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    12901 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    12961 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    13021 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    13081 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
    13141 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    13201 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    13261 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    13321 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    13381 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    13441 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    13501 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    13561 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    13621 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    13681 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    13741 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    13801 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    13861 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
    13921 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
    13981 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    14041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    14101 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    14161 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
    14221 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    14281 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    14341 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    14401 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    14461 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    14521 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    14581 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    14641 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    14701 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    14761 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    14821 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    14881 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    14941 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    15001 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    15061 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
