LOCUS       sequence-353516-       13981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0323
FEATURES             Location/Qualifiers
     source          1..13981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6919..7507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6972..6992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7390..7401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7579..7812
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7890..8011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7927..7946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7981..8000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8051..8186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8206..8225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8207..8225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8235..8252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8235..8251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8244..8266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8393..8848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8480..8499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8690..8711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8874..8978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8979..9047,9048..9839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9197..9216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10010..10598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10499..10518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10752..10769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10886..10907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10922..10927,10928..10945,10946..10952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10960..10976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10965..10987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10984..11000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10984..11000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11019..11039
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11021..11039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11067..11293
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11294..11474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11521..11646
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12139..12372
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12557..12601
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12832..13042
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12854..13050
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12901..13036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13082..13684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13082..13101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13578..13598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13727..13844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 caagggaaga gagagaatgg ccgctgccgc cgccgctgct gccgccgccg ccgccgccgc
     1261 tcagtgccgg agccctcggt gcgcggcgga gagaagagga ttccggcggg aactcgactc
     1321 ttggcgccac cgcctcatgc actgtgtagg ttttgagagt attttagaag ggctttatgg
     1381 accacggcta cgaagagacc tcagtttatt tgaagactgt gaaccagaag agctgactga
     1441 ctggtctatg gatgaaaaat gttcattttg taacctacag agagaagcag tcagtgattg
     1501 tataccatct cttgattctt cacagtcaac accaacagag gagctatcat ctcagggcca
     1561 gtccaacact gataagattg aatgccaagc agaaaattac ctaaatgcac tctttcgaaa
     1621 gaaagatctt cctcagaact gtgatcctaa cattccccta gttgctcagg aattaatgaa
     1681 aaagatgata cgtcaatttg cgattgagta catttcaaaa agtggtaaaa ctcaagagaa
     1741 tagaaatggt tcaattggac caagtatagt atgtaaaagt atccaaatga atcaagcaga
     1801 aaactccctt caggaagagc aggaaggccc cttagacctc actgtgaatc gaatgcaaga
     1861 acaaaatact cagcaagggg atggagtgtt agatctctct acaaagaaaa ccagcataaa
     1921 atctgaagag tcatccatat gtgatccttc ttctgaaaat tcagtggctg gttcaactgt
     1981 tgatgcaaaa tcagaggaag ctactaaaat ggaaaaaaga aaatcagcat taaacaaagt
     2041 tttggaatct ttgtgcatac atcaccagca acaagttttg gctatgttga aatttctagt
     2101 ccaagagcag aatgctgctt ctctttgctg ttgtaataca tcatgtgttg tgtcttcaga
     2161 atctcaaaag cccttaatcg aagatgattt atgtggtctg ttctgtagtt gtgaatatag
     2221 gctggcagaa agaggttgtt tacaaaacga aaaacaaagc cctggtttag aacctctgcc
     2281 agtctgtatt aaagatttac attgtttatc ttgccaaact gtaactgttg aacacgttaa
     2341 gccagtagtg aatagaggaa ttgcagacag ttataattct cacaggtgct gttctggact
     2401 gttaccaaac attcactcta caaaatcagc ctttcgtagt cctcttttgt caaaggaagt
     2461 atgtgatctt tcagtcactc ttaaagatgc ctgtagatct cgaagtccct cacccccacc
     2521 attatcacct atagaaactg aaggatttga aaaattgaaa gacgtcatct cagagatttc
     2581 agccttagaa aataacaaac ttgaagcaaa cattaaccag cctccatctc tcacaccagc
     2641 agaaatcagc agcaataaga gtgatcatca agataaaata cttaaaacta aaaaatccag
     2701 taactcttat tctttactct caaatgacag caataattct actacaaatc atgaaaaagg
     2761 tgaaactgct gtaatttttc aagatttaat ggatcgcatt aatgaaaaac taaaatcaat
     2821 agaaaccacg gatatgacaa gccttgtaaa attatctagc agtgattata atacatataa
     2881 tgatttaaaa ttgggagatt tcataacatc tctcttgcat aatgcaaaag ccagtgatta
     2941 tagttttatg gaattattga gtcaacatga taaaaaggta gaaaataaaa ttattcagac
     3001 aagatttcga aagcgtcaag aaactttatt tgcaatgcgc aactcctctg attcacccat
     3061 gtttagaagg cagtctttac agataaaaag agaacttgct agtcttgatg aaaattttac
     3121 aagaaaaaaa tacaccgaaa aaagttcaag gaaattgaca caaaacaatg agatatcttc
     3181 atcagacaaa ggagaattct atcatgacca agggccttct ttacaaaatt ctaaaagact
     3241 tcaaggtaaa aatcatgctg aaacatcatt ttcaccaaat tatgcattac agtcactgca
     3301 actacctctt catagttcag aaactaactt gtcttttaat gaattttcag aaagctttaa
     3361 aacaacttcc cctgagaaaa tgagcataag aaagccacag gagaaatctg cagatggaaa
     3421 acaatttttg caaaatgata ggaaaaatcc aaagttaggt aatactcaaa ctcttttgag
     3481 aaatgatgtt tctggacttt tgagcagaac taaacgaaat attgtgcctc cagggtggta
     3541 ttctgtatat gtaacaaata attatgtttt taaaaaatcc cctaaagcca aaaaagtatc
     3601 cgaatccaca acaaaaaacg atccagtgaa aaatattcac attgaaagct cacacaatat
     3661 agacctaaac aaaattgcaa tgaattctaa tttacaagtg gttgtgaagc gtttggaaga
     3721 tacaataagt atagccaaaa aatcctggaa taatcagcca ttatcagaag gatataaagc
     3781 atccaagaaa ttgatagaaa ttgatggtaa agaccaacat gctgacagaa atatgactct
     3841 tactctaaat agaatgacat gcaaagaaca gagcttatca aaatctgtgg tagcatccgg
     3901 caatattatc aatagtcact gcatgcctac agtggatttg aataacaaaa gacttgaaaa
     3961 tctgaaaaag tcatctattt tagatatggg tcgcttgatt tccagtgttg aaaatgtacc
     4021 agcaaaatat gaaggtactg aaagttcatc tgtttccaac tattctagtc ctatcaaact
     4081 catgttttta tctgaggtta aaagtagtga aggggtcaaa tatactttaa cttcagttgg
     4141 cacttcccat tcaaatgttg ttctcccttc tgaaaaacct acaacccatc acgtaactga
     4201 agaaaaaaca gaaacaaatg aggatatctc aaatgcgaac tctgaaaatt atcactccag
     4261 tcattatgat actgatactt ttcaaagaga actaaacaaa ttcagtcatg caaaagaaac
     4321 tgcaggatcc tctacaatgt ttataggtga tataaatagt gataagccac aagaagaacc
     4381 taaggacaat tcaagcagtg ctactgatcc atcttttaaa agaaaaccag gtagaccaaa
     4441 aaaaataggt ccccaagttg tgaaacagat taagcgacca attggaagac caccaaagcc
     4501 taaaattgat caaacaaaca tcactgtttg ccagaatgag ccctttagtg ctggaaggaa
     4561 aagcccagaa tgtctcatat cagaagtaaa agaaggtatt tataaaaaga gtattacagt
     4621 aactgttatt tacggaaggt caagaagaac taaaaggcat gtttctgaag gaagggtaaa
     4681 cataagcaac cttatgtctt taaacaataa tgctggtgat tttccaactg aatataatag
     4741 tcccagaaat attagtgaag acaaaattga cttgggtgaa agaataagtg ctgtagcaag
     4801 cttgactact gaaagtgaga tcttggggtc tggctttgaa tatgtgagac ccatcaagaa
     4861 caaatctgtg atacctcagc cttccaagaa cattattcga ccaaatcaga agcctttggc
     4921 aataagtagg aagcctggta gaccggcaaa agtgaaaatc tctggcatat ctgtgactat
     4981 taatagaact tcacctcagg aaagagaagt aagtattagc agctgcttgc ctcctttaga
     5041 acaggataat acgtcaggaa aaaatctgcc tgaagaaaag tatgaccaac agtgcactca
     5101 aatggataaa ataaggcaca ctgaagctga tatatttaaa aatggatcaa aacgtatgat
     5161 tgctactgta cctttgagac attctattag ggatagaaaa ccatctctgc atttcttaca
     5221 ttcattagca tcttctagct cacttattta tagaaacgct ctgctccata aatcatataa
     5281 actgcatttg cagaaaaata aaagtcagaa ggaaaaacat aggcactcaa agatgaaaat
     5341 agcttacaaa gataccccaa gaaacagact ttcgcggaat gcaaaaaagt gtttggaaga
     5401 taataaatta gtacctattt ctgaagtatc cttggatcct ataatttcat caaacccttt
     5461 gctcaggtgg tgggctactt ctgcttcaaa tgattcctta ttagaggaat taaacaatag
     5521 atttgagcaa ataacaaatg cttgggtgca agtaagtgga gatgaagctg aaaattgtat
     5581 tcataaaaaa agagaacaca ttgaaaatga tcatttcaaa gtagcaagcc ctttggaaac
     5641 ttgtctttta gaacttgaag tttcacctgt aaaaatgctt tttcagaaaa agtatgattt
     5701 gaatgaactc tgtacttggt ttatgcaaac aacagaaaca cagtctcttt cactagttag
     5761 aaaagcaaat gcccgaaacc ctttggaagt aataaatacc aggggaatca aattagggac
     5821 caaatattct gactttaatg ccagcccctt cagaaagcac tttaaaaaat ttgcactctc
     5881 ttctccttca aaatcagcag agaaattgca tatactgcat aaagtggcta actctccact
     5941 cttaaatgtg aaaagtaatt tagcaatagc tagattaaaa aggactgagt ttaagaggtt
     6001 gcatcatgaa aggtggaaaa gagagggaaa gctgcacaac catggaacag ttgactggaa
     6061 ctctaaaagg agaaacttaa gatttttctg ccagaaccaa tttttaaata agactgaggg
     6121 agaaacaaat gctgacatcc cactccaagg aaaaagcata gtagataatc agtgtgtttt
     6181 gccacctgag atcaggggtg acttgcagca gagggtagta atgcctgact tcaaaataca
     6241 tgctagtttt gagaataaat ttaagtcaga agcaaaagag aatggaacaa attgcagcca
     6301 aaaagacttt caaaagggac caagactaga aaatgtatgt cctaatagtt ggaggtcaaa
     6361 aaccttaaaa gactgtagaa tatttttgag gaagctcaac tgtcttgaac acagaaatac
     6421 ttttaagcta aatacaatca tttactctcc tgaatctact gacagtggaa atactcatca
     6481 gactcatatg gaagaatcaa agcgctttac tttaagatct cattctgcta ggcaaaattc
     6541 ttttaaaaag caatctaaag aaatagaaaa tgctaacaca aataatcctt cagctgatga
     6601 atttgctgac catcttggca atagtaaatt aagcaaatgt gttaactttg acaagaatcc
     6661 tgatagtttt gaagttctta gcaatttgaa caaaagaaaa agaccaccat ggaagatcac
     6721 agaaatgtca acaaaaagac ataaacgaca gtcttgcaac agtggacaaa tggcaaacta
     6781 tttttccaaa tccctagcat gctacaagta gtaatgaact agtccgtaac ttgaaagtat
     6841 ttcgatttct tggctttata tatcttgtgg aaaggacgag ttatcgggcc tcgcgggatg
     6901 cgcacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6961 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7021 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7081 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7141 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7201 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7261 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7321 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7381 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7441 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7501 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7561 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7621 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7681 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7741 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7801 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7861 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7921 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7981 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8101 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8161 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     8221 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8281 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8341 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8401 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8461 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8521 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8581 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8641 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8701 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8761 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8821 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8881 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8941 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9001 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9061 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9121 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9181 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9241 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9301 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9361 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9421 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9481 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9541 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9601 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9661 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9721 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9781 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9841 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10141 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10801 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10861 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10921 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10981 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    11041 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
    11101 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
    11161 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
    11221 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
    11281 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11461 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11521 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    11581 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    11641 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    11701 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    11761 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    11821 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    11881 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    11941 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    12001 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    12061 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    12121 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    12181 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    12241 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    12301 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    12361 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    12421 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    12481 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    12541 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    12601 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    12661 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    12721 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
    12781 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    12841 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
    12901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    12961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    13021 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
    13081 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    13141 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    13201 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    13261 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    13321 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    13381 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    13441 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    13501 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    13561 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    13621 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    13681 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    13741 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    13801 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    13861 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    13921 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    13981 t
//
