LOCUS       sequence-353517-       12061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0542
FEATURES             Location/Qualifiers
     source          1..12061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4999..5587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5052..5072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5470..5481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5659..5892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5970..6091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6007..6026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6061..6080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6131..6266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6286..6305)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6287..6305)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6315..6332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6315..6331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6324..6346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6473..6928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6560..6579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6770..6791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6954..7058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7059..7127,7128..7919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7277..7296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8090..8678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8579..8598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8832..8849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8966..8987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9002..9007,9008..9025,9026..9032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9040..9056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9045..9067
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9064..9080
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9064..9080
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9099..9119
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9101..9119
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9147..9373
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9374..9554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9601..9726
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10219..10452
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10637..10681
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10912..11122
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10934..11130
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10981..11116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11162..11764
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11162..11181)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11658..11678
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11807..11924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtg
     1201 taaatgttgt aacctcttct caccaaatca gtcggaactc ctctcccacg tttcagagaa
     1261 gcacatggaa gaaggggtta atgttgatga gattattatt ccccttaggc ctctgagtac
     1321 acctgaaccc cccaactcaa gcaaaaccgg agatgagttt ttggtcatga agaggaagag
     1381 aggcaggcct aaggggtcca cgaagaagtc cagcacagaa gaggagctgg cagaaaacat
     1441 cgtgagtccg actgaggaca gcccgctggc tccggaggaa gggaacagcc tgcctccaag
     1501 cagcttggag tgtagcaagt gctgtcggaa gttctccaac acgcgccagc tgcggaagca
     1561 catctgcatt atcgtgctga atttgggtga ggaggaagga gaagcaggta acgagtctga
     1621 ccttgaacta gaaaagaagt gtaaggaaga tgatcgggaa aaagcctcga aaagaccacg
     1681 gtcacagaaa acagagaaag tccagaagat ctcaggaaag gaggccagac agctttctgg
     1741 ggcgaagaaa cccatcataa gtgtggtttt aactgcacac gaagcaattc caggtgctac
     1801 caagattgtg ccagtggagg ctgggccccc tgaaacagga gctacaaatt ctgagaccac
     1861 ttcagcagac ctggtgcctc ggagaggcta ccaggaatac gccattcagc agacacctta
     1921 tgagcaacca atgaagtcaa gcaggctagg tcccactcag ctcaaaatct tcacttgtga
     1981 atactgcaac aaggtcttca agttcaagca ctcgctgcag gcccacctga ggatccacac
     2041 caatgaaaag ccatacaagt gcccccagtg cagctatgcc agtgccatca aggccaacct
     2101 caatgtgcac ctgcgcaagc acactggaga gaagttcgcc tgcgactatt gctcgttcac
     2161 ctgcctgagc aagggccacc tcaaggtgca catcgagcga gtgcacaaga agatcaagca
     2221 gcactgccgc ttctgcaaga agaagtactc tgacgtcaag aacctcatca agcacatccg
     2281 agacgcgcat gacccacagg acaagaaggt caaagaggcc ttggacgagc tctgcctgat
     2341 gacgagggag ggcaagcggc agctgctcta tgactgccac atctgtgagc gcaagttcaa
     2401 gaacgagctg gaccgtgacc gccatatgct ggtccacgga gacaagtggc cttttgcctg
     2461 tgagctctgt ggccatgggg ccaccaagta ccaggcgctg gaactgcatg tcaggaagca
     2521 ccccttcgtg tacgtctgtg ccgtctgccg caagaagttc gtcagctcca tcaggctgcg
     2581 cacccacatc aaagaggtgc acggggctgc ccaggaggcc ttggtcttca ccagttccat
     2641 caaccagagc ttctgcctcc tggaacctgg tggggacatc cagcaagaag ctctggggga
     2701 ccagctacag ctggtggaag aggagtttgc cctccagggc gtgaatgcac tcaaggaaga
     2761 ggcctgtcct ggggacactc agctggagga gggccggaag gagccggagg cccctgggga
     2821 aatgcctgcc ccagctgtgc acctggcctc cccgcaggcc gaaagcacag ccctgccacc
     2881 ctgtgagctg gaaaccaccg tggtctcctc ctcagacctg cattctcaag aggtggtttc
     2941 agatgatttt ttgttgaaaa atgatacctc ctccgcagag gctcatgctg ctcctgagaa
     3001 gcccccagac atgcagcaca gaagctcagt ccagacgcaa ggtgaagtga tcacactact
     3061 gctgtccaag gcccagagtg ctgggtcaga tcaggaaagc catggcgccc agagccccct
     3121 aggggaaggg cagaacatgg ctgtgctttc agctggtgac ccagatccca gcaggtgtct
     3181 caggtcaaac ccagctgagg cctcagacct cctccctcca gtagctggtg gtggggacac
     3241 catcacacat cagcctgact cttgcaaagc tgcccctgag caccggtcag gcatcaccgc
     3301 tttcatgaag gtcctgaaca gtttacagaa gaagcaaatg aacaccagct tgtgtgagcg
     3361 gatccggaag gtttatggag acctggagtg tgaatactgt ggcaaacttt tttggtacca
     3421 agtgcatttt gatatgcatg tccgcaccca cacccgggaa catctgtatt attgctctca
     3481 gtgtcattat tcttccatca ccaaaaactg ccttaaacgc cacgtaattc agaaacacag
     3541 taacatcttg ctgaagtgtc ccaccgatgg ctgtgactac tcaactccag ataaatataa
     3601 gctacaggca catcttaaag ttcacacagc actggacaaa aggagttatt cttgtcctgt
     3661 ttgtgaaaag tctttttcag aggatcgatt gataaagtca catatcaaga ccaaccatcc
     3721 tgaggtctcc atgagcacca tttctgaggt tctcgggagg agggttcagc tgaaagggct
     3781 aattggaaag agagccatga aatgcccata ttgtgacttt tatttcatga agaatggctc
     3841 agaccttcag cgtcatattt gggctcatga aggtgtgaag cccttcaagt gttctttgtg
     3901 tgagtatgca actcgtagca agagtaacct caaggctcat atgaatcgtc acagcactga
     3961 gaaaacccac ctatgtgaca tgtgtggcaa gaaattcaaa tcaaaaggga cactgaaaag
     4021 tcacaaactc cttcacactg cagatgggaa gcagtttaag tgcacggtgt gtgactacac
     4081 agcggcccag aagccacagc tgctgcggca catggaacag catgtctcct tcaagccttt
     4141 ccgctgtgcc cattgccatt actcctgcaa catatctggc tctctgaagc ggcactacaa
     4201 caggaagcac cctaatgagg agtatgccaa cgtgggcacc ggggagctgg cagcggaggt
     4261 gctcatccag caaggtggtt tgaagtgtcc tgtttgcagc tttgtatatg gcaccaaatg
     4321 ggagttcaat aggcacttga agaacaaaca tggcttgaag gtggtggaaa ttgatggaga
     4381 ccccaagtgg gagacagcaa cagaagctcc tgaggagccc tccacccagt atctccacat
     4441 cacagaggcc gaagaagacg ttcaagggac acaggcagcg gtggccgcgc tccaggacct
     4501 gagatacacc tctgagagtg gcgaccgact ggaccccacg gccgtgaaca tcctgcagca
     4561 gatcattgag ctgggcgccg agacccatga cgccactgcc cttgcctcgg tggttgccat
     4621 ggcaccaggg acggtgactg tggttaagca ggtcaccgag gaggagccca gctccaacca
     4681 cacggtcatg atccaggaga cggtccagca agcgtccgtg gagcttgccg agcagcacca
     4741 cctggtggtg tcctccgacg acgtggaggg cattgagacg gtgactgtct acacgcaggg
     4801 cggggaggcc tcggagttca tcgtctacgt gcaggaggcc atgcagcctg tggaggagca
     4861 ggctgtggag cagccggccc aggaactcta gtaatgaact agtccgtaac ttgaaagtat
     4921 ttcgatttct tggctttata tatcttgtgg aaaggacgat gggacaactc cgcaaagccg
     4981 cctacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     5401 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5521 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5581 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5641 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5881 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     5941 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6121 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6181 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6241 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6301 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6901 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9061 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9121 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9181 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9241 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9301 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9361 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9421 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9481 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9541 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9601 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9661 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9721 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9781 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     9841 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     9901 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     9961 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10021 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10081 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10141 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10201 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10261 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10321 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10381 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10441 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10501 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10561 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10621 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10681 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10741 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10801 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
    10861 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10921 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
    10981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    11041 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    11101 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
    11161 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    11221 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    11281 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    11341 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    11401 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    11461 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    11521 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    11581 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    11641 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    11701 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    11761 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    11821 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    11881 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    11941 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    12001 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    12061 t
//
