LOCUS       sequence-353518-       13348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13348)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2010
FEATURES             Location/Qualifiers
     source          1..13348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6286..6874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6339..6359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6757..6768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6946..7179
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7257..7378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7294..7313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7348..7367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7418..7553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7573..7592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7574..7592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7602..7619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7602..7618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7611..7633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7760..8215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7847..7866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8057..8078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8241..8345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8346..8414,8415..9206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8564..8583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9377..9965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9866..9885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10119..10136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10253..10274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10289..10294,10295..10312,10313..10319)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10327..10343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10332..10354
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10351..10367
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10351..10367
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10386..10406
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10388..10406
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10434..10660
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10661..10841
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10888..11013
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11506..11739
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11924..11968
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12199..12409
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12221..12417
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12268..12403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12449..13051
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12449..12468)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12945..12965
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13094..13211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgat
     1201 ggatagtgag aataaacccg aaaatgatga ggatgaaaag ataaacaaag aagcacaaga
     1261 cttgacaaag ctttcatccc ataatgaaga cggtgggcct gtatctgatg tgatagcaag
     1321 tttccctgag aattctatgg gcaaaagagg tttttcagaa tcatcgaact ctgatagtgt
     1381 tgttatagga gaagacagaa ataaacatgc ttccaaacgc aggaaattag atgaggcaga
     1441 gccccttaaa tctggaaagc aaggtatttg tagattagaa acttctgaga gctcagtcac
     1501 agaagggggt attgcattag atgaaacagg gaaggagacc tttctgagtg actgcacagt
     1561 tggaggcaca tgtctcccaa atgccctctc cccttcttgc aattttagca ctattgatgt
     1621 tgtttctctg aaaacagaca ctgaaaaaac atctgctcag gaaatggttt cccttgatct
     1681 ggaaagagaa tctcctttcc ccccgaaaga aattagtgtt agttgtacca ttgggaatgt
     1741 agatacagtt ctcaaatgca gcatctgtgg gcatttgttt tcttcttgct ctgacttgga
     1801 aaaacatgct gagtctcaca tgcagcagcc taaggaacat acctgttgtc actgcagcca
     1861 caaagcagag agcagctcag cactacatat gcatatcaaa caagcacatg ggccacagaa
     1921 ggtcttttcc tgtgatcttt gtggttttca gtgttcagaa gaaaacttgt tgaatgcaca
     1981 ttatcttggc aaaacacatc tccgtcgtca gaatctggct gctcgtggag gatttgtaca
     2041 gatcttaaca aaacaacctt ttcctaaaaa atcacgtaca atggcaacaa aaaatgttca
     2101 ctcaaaacca agaacttcta aatcaatagc aaagaatagt gattcaaaag gattacgaaa
     2161 tgtgggaagc acgtttaaag atttcagagg aagtatttct aaacaaagtg gtagtagcag
     2221 tgagcttctt gttgaaatga tgccttccag aaatactttg tcacaggaag tagagattgt
     2281 tgaagaacat gttacttccc ttggtctagc tcagaatcct gaaaaccaga gtagaaagct
     2341 agacacctta gtaacctcag agggtctctt agagaaattg gaatctacaa aaaataccct
     2401 tcaggcagca cacggtaaca gtgtaacctc gaggccaaga cctgagcgaa atattctcgt
     2461 gttgggtaat agctttcgtc gacgaagcag cactttcacc ttgaagggcc aggcaaagaa
     2521 aaggtttaat cttttaggaa ttaaaagagg tacaagtgaa actcagagga tgtatatgaa
     2581 acacttgaga acacagatga aaacacacga tgcagaatca gtgctgaaac acctggaagc
     2641 gtgcagcagt gtgcagagag tgtgtgtgac tacctcagaa acccaggagg cagagcaggg
     2701 ccaggggagt gcccgtcctc cggactccgg gctgcattcc ctgacagtga agccagcttc
     2761 tggctctcag acgttgtgtg cttgtacaga ctgtgggcaa gtagctacaa ataggacaga
     2821 tttggaaatc catgtgaaaa ggtgccatgc cagagagatg aaattttact gccgtacttg
     2881 tgacttctct agtatgtcaa gaagggactt agatgaacat ttgcacagta accagcatca
     2941 gcaaactgct tctgtcctga gttgtcagtg ttgttcattt atatccttgg atgaaataaa
     3001 tcttagagac cacatgaagg aaaagcacaa tatgcatttt ctttgcaccc cttgtaatct
     3061 gttctttttg tctgaaaaag atgtggaaga acacaaagcc accgagaagc atattaattc
     3121 attggttcaa ccaaagactt tgcaatcatc taacagtgat ttggttttac agactttacc
     3181 tttgagtact ttagaatcag aaaacgcaaa agagtctatg gatgactcag gaaaagcatc
     3241 tcaggaagaa cctctgaagt ccagggtaag ccatggtaat gaagtgaggc attccagtaa
     3301 gcctcagttt cagtgtaaga agtgttttta taaaacaaga tcttctactg ttctcacgag
     3361 acatataaag cttcggcatg gtcaagacta tcattttctt tgtaaagctt gtaatcttta
     3421 ttcattgagc aaagaaggaa tggagaaaca cattaaaaga agcaagcatc ttgaaaatgc
     3481 taagaaaaat aatattggct taagctttga agaatgtatt gaaagggtat gtataggtgc
     3541 aaatgataaa aaagaagagt ttgatgtttc cggaaatgga aggattgaag gccatatagg
     3601 tgtgcaatta caagagcatt cctatcttga gaagggcatg ctggcgtctg aggaactgtc
     3661 acagtctggt ggtagcacca aagatgatga attagcttca accactactc caaagagagg
     3721 gagacctaaa ggtaacatct cacggacgtg ttcacactgt ggccttttgg cctctagtat
     3781 tacaaacttg actgttcaca ttagacgaaa acacagtcac cagtatagtt atttatgcaa
     3841 agtgtgtaag tattacactg taactaaggg agatatggaa cgtcattgtg ccaccaagaa
     3901 acataaagga cgggtagaaa tagaagcaag tggaaaacac agttcagata tcattgttgg
     3961 ccctgaaggg ggtagccttg aagctggtaa aaagaatgct ggctcagcag tgaccatgtc
     4021 agatgaacat gctaacaaac cagctgagtc acccacctcc gttttagaga agccagatcg
     4081 tggaaactca attgaagctg aagttgaaaa tgtatttcat tctctagatg gagaagttaa
     4141 cagccatctt cttgataaaa aggagcaaat atcttcagag ccagaggact tcgcccagcc
     4201 gggggatgtg tactcccaga gagatgttac aggcacaggt gagaataagt gtttgcactg
     4261 tgagtttagt gctcactcct ctgcttctct agagctgcat gtaaaacgga aacatacaaa
     4321 agagtttgag ttttattgca tggcatgcga ttactacgcg gtgactcgtc gcgagatgac
     4381 caggcatgca gcaacagaga agcacaaaat gaaaaggcag tcttatctca actctgctaa
     4441 tgtagaagct ggttctgcag acatgtccaa aaacatcatt atgcctgaag aagagcatca
     4501 acaaaattct gaggaatttc aaataatttc aggtcaacca tctgatactc ttaaatctag
     4561 aaatgctgca gattgctcta ttttaaatga gaatactaat ttagatatgt ctaaagtgct
     4621 ctgcgctgct gactctgtag aagttgagac tgaagaagaa tctaatttca atgaagacca
     4681 ttccttttgt gagactttcc aacaggctcc tgtcaaggat aaagttagga aacctgagga
     4741 gatgatgtca cttactatgt cctcaaacta tggctcccca agcagatttc aaaatgaaaa
     4801 ttcaggaagc tctgccttaa attgtgagac agcaaagaaa aaccatgaga tatcgaatga
     4861 tgcaggtgag ctgcgtgtcc attgtgaggg tgaaggagga aacgcaggag acggtggagg
     4921 tgttgtcccc cacagacacc tgtgccctgt gacgctcgat ggggagcgct cggctgaaag
     4981 ccctgtgctc gttgtgacaa gaataaccag agaacaggga aatctggaga gcgggggtca
     5041 gaacagagtt gcacgtgggc atggtttgga agacttgaaa ggtgtccaag aagatcccgt
     5101 tctggggaat aaggaaattc tgatgaattc acaacatgaa acagaattta ttttggagga
     5161 ggatggccca gcttctgata gcacagttga aagtagtgat gtctatgaaa ctataattag
     5221 tattgatgat aaagggcagg ccatgtacag ttttggtcga tttgactcct ccataataag
     5281 aataaagaac cctgaagatg gtgagttgat agaccagtct gaagagggct tgatagcaac
     5341 gggagtgaga attagtgagc tgcccttgaa agactgtgct caaggtgtga aaaagaagaa
     5401 atctgagggc agttccattg gtgagtctac acgaattcgc tgtgatgatt gtggcttctt
     5461 agcagatgga ctgagtggac tgaatgttca catagccatg aagcatccta caaaagagaa
     5521 gcacttccat tgtttactct gtggaaagtc gttctatacc gaaagcaacc ttcaccagca
     5581 tctggctagt gccggccaca tgagaaatga gcaggccagt gtggaggagc ttccggaggg
     5641 aggggccacc tttaaatgtg tcaagtgcac agagcccttt gattctgaac agaatttatt
     5701 tttacatatt aaaggacagc atgaggaatt gctgcgggag gtgaataagt atatagtgga
     5761 agacactgag caaatcaacc gcgagaggga ggaaaaccag ggaaacgtct gcaagtattg
     5821 tgggaagatg tgtcgaagca gcaactcgat ggccttcctg gcacacattc gcactcacac
     5881 aggatcaaaa ccattcaagt gcaagatatg ccattttgca acagctcagc ttggagatgc
     5941 cagaaaccat gtgaaaaggc accttgggat gagggaatac aagtgtcatg tctgtggggt
     6001 tgcttttgta atgaagaagc acttaaatac tcatctacta ggcaagcatg gagttggcac
     6061 cccaaaagaa agagtaaagg ttgcatatcg aaagatcggg acgttgccag gaatacagaa
     6121 caacaggaac gcgtcgagtg cctctgaagc ccaaagtctt tgtgagcact tttcatagta
     6181 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     6241 ggacgaatcg ccaggagaca cgtagttcat acgcgttaag tcgacaatca acctctggat
     6301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6841 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     6901 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6961 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     7201 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7261 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7321 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7381 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7441 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7501 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     7561 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     7621 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7681 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7741 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8101 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8161 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     8221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10081 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10141 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10201 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10261 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10321 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    10381 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
    10441 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
    10501 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
    10561 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
    10621 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
    10681 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10741 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10801 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10861 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10921 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10981 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    11041 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    11101 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    11161 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    11221 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    11281 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    11341 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    11401 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    11461 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    11521 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    11581 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    11641 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    11701 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    11761 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    11821 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    11881 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11941 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    12001 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    12061 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    12121 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
    12181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
    12241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    12301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    12361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    12421 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    12481 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    12541 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    12601 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    12661 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    12721 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    12781 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    12841 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    12901 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    12961 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    13021 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    13081 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    13141 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    13201 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    13261 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    13321 cttctaggtc ttgaaaggag tgggaatt
//
