LOCUS       sequence-353550-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  K9 (vIRF1) TAGGED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0331
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1448..1513
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1534..1558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1638..1662)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agacccaagc tggctagtta agcttgatca aacaagtttg tacaaaaacg caggctcttt
       61 aaaggaacca attcagtcga ctggatccgg taccgccacc atggacccag gccaaagacc
      121 gaaccctttt ggggcgcctg gggcgatacc caaaaaacct tgtttgtcac aagggtcacc
      181 gggaacatct ggctccgggg ctccttgcga tgaaccatcc aggtcggagt caccgggaga
      241 aggaccctcc gggaccgggg gttctgcagc cgcaggcgat attacacggc aggctgtggt
      301 ggctgcgata acggagtgga gccggacacg acaactaaga atatctaccg gtgccagtga
      361 ggggaaagcg tcaatcaagg attggatagt atgtcaagtc aacagtggaa aatttcccgg
      421 agtagagtgg gaagacgaag agcgcacccg cttccgtata ccagttacgc ccctcgcgga
      481 cccctgtttt gaatggcgca gagacgggga attgggtgtt gtgtatatcc gcgaaagggg
      541 taacatgccc gtggacgcct cgtttaaagg caccaggggg cgcaggcgaa tgcttgccgc
      601 gctacgcagg acgcggggtt tacaagagat tggcaaaggc atttctcagg atggacacca
      661 ttttctggtc tttagagtac gtaaaccgga ggaagaacaa tgcgtggaat gtggagtcgt
      721 ggccggagcc gtccacgatt ttaacaatat ggcacgtctc ttgcaagagg gatttttttc
      781 cccggggcag tgcttgccag gcgagattgt aacgcccgtg ccatcttgta cgacggcaga
      841 gggtcaggag gccgtcattg actggggtcg gttgtttatc aggatgtatt ataatggcga
      901 acaggttcat gagttactga cgacaagcca gtcgggctgt cgaatctcat cggccttgcg
      961 tagggacccc gcagttcatt actgtgcagt ggggtctccg ggccaggtat ggctacccaa
     1021 tgtgccaaac ctggcctgcg agatagccaa gcgggagcta tgcgatacgc tggacgcatg
     1081 tgcaaaaggc attctgctga ctagctcttg taatggcata ttttgcgtat gttatcataa
     1141 cgggcccgtg cactttattg gaaataccgt tccgcctgac tccggccccc tcttgctgcc
     1201 ccagggaaaa ccgacgagga tatttaaccc gaatacattt ctggtggggt tggcaaattc
     1261 accgttacca gcaccctctc acgtgacgtg tcctttggtg aaactgtggc tggggaaacc
     1321 ggtggcggtt ggcaagttag agccccacgc cccgtccccg cgagactttg cggcgaggtg
     1381 cagtaatttc tcagacgcct gcgtcgtact cgaaataatg ccgaagccgt tatgggatgc
     1441 catgcaagac tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga
     1501 tgacgatgac aagtagtaac tcgagatatc tagacccagc tttcttgtac aaagtggttc
     1561 gatctagaat ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1621 aattaggaga tatctagacc cagctttctt gtacaaagtg gttcgatcta gaatggctag
     1681 caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1741 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgctacat acg
//
