LOCUS       sequence-353551-        2027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  K10.5 (vIRF3) tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2027)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0335
FEATURES             Location/Qualifiers
     source          1..2027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1712..1777
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     1887..1906
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cggtaccgcc accatggcgg gacgcaggct tacctggatt tctgagttta ttgtaggtgc
       61 tttggactct gataaatatc ctttggtcaa gtggctagat agatctactg gaacatttct
      121 tgctccggct gcccgtaatg acgtaattcc tctggatagc ctacagtttt tcattgattt
      181 taagagggaa tgcctatcga agggcctgca tcccagagat ttactgggct cgccgattac
      241 ggcttttggg aaaatatgta ccacgtcgcg gcgccttaga cgcttgccag gtgaagagta
      301 cgaggtcgta cagggaatta attgtagaag gtggcgcctc ctgtgtgccg aggtaaagga
      361 atgctggtgg tgcgttcatg ccaggactca cctacacagt gggtcatcac tatgggaaat
      421 tttgtatcaa cacagtgtac ggctcgagaa gcatcggaga agaccaaggc catttgtggg
      481 tgaaaactcg gattccagtg aggaggatca cccagccttt tgcgatgtgc cggtcacgca
      541 gacgggcgcg gaatctgagg actctggaga cgagggacca tcgacgcgcc atagtgcgtc
      601 tggggttcag ccagttgatg atgccaatgc cgactctcct ggctctggag acgaaggacc
      661 ctcgacgcgt catagcgact cgcagccccc cccggccaat gaaacaacgg tgcacacaga
      721 caacgttgaa gatgacctca cactgcttga taaagaatct gcatgtgcat tgatgtacca
      781 cgtgggacag gagatggaca tgctaatgag ggcgatgtgc gatgaagacc tctttgatct
      841 gcttggcatc ccagaggatg ttatcgcaac atcacagccc ggaggcgaca cggatgcaag
      901 cggcgtggta acagagggct caatcgccgc ctcggctgtc ggggcgggtg tagaggatgt
      961 gtacttagct ggggcactcg aggcccagaa tgtagcaggg gaatatgtgt tggagataag
     1021 tgacgaagaa gtcgatgatg gtgctggact gccgccggcg tccagacgcc ggccagttgt
     1081 tggcgaattt ttatgggatg atgggccacg gagacacgag aggcctacca cgaggcgcat
     1141 tcgccacagg aagcttagat ccgcatatta tagagtggca cggccgccag taatgataac
     1201 cgataggctt ggtgtggaag tgttttattt tggccgccct gcaatgtctt tggaagtgga
     1261 acgaaaggtg tttattctat gttcccagaa cccactggca gacattagcc actcttgctt
     1321 gcattcgcgc aaagggttaa gagttttgtt gcccaaacct gacgacaata acacagggcc
     1381 aggagacgtt aacctgctgg cggccgtgct gcgctcgttt gcttcgggtc ttgtgatagt
     1441 ttctctccga tctggcattt atgttaagaa tttgtgcaag tctaccgtat tatatcatgg
     1501 aaataatcct ccaaagaagt ttggtgtgat ctgcggactg tcatctaggg ctgttctgga
     1561 tgtttttaat gtggcacaat atcgcataca gggacatgag cacattaaaa aaacaactgt
     1621 gttcatcgga ggtgacccaa cgtcggcaga acagttcgat atggtccccc tcgtcatcaa
     1681 gctcagattg cgttcagtta catgtgatga cgactacaaa gaccatgacg gtgattataa
     1741 agatcatgat atcgattaca aggatgacga tgacaagtag taacccgggc gacggatggt
     1801 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
     1861 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     1921 ctccgttatc ggggaagaag tggctgatcg ggcgacggat ggtgatcccc ctggccagtg
     1981 cacgtctgct gtcagataaa gtcccccgtg aactttaccc ggtggtg
//
