LOCUS       sequence-353552-        3086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orf 63 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3086)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0339
FEATURES             Location/Qualifiers
     source          1..3086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2847..2912
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     3022..3041
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgtacaaaa aagcaggctc tttaaaggaa ccaattcagt cgactgatcc ggtaccgcca
       61 ccatggacgg cacagacgct cttgaaaagc ttaccaaagg cctgagtggg ggaggcggga
      121 gccttcacca gacaaagctg ttgatggaat ttcaactccg aggactgccg gtgcctgccc
      181 tcttaaacag cagcacaaca gagcagtttt taaatactgt tgcccaactg ccgacggacc
      241 tatcaaaatt tatacgcgac tatcgcgtgt tcgcactggt tcgcgcggcg tattttttag
      301 aacccccttc tagcatcgac ccccttgagg cagcgcgcgc tcttggacgc ctggttgata
      361 tattatcatc acaaccaccg cagaacaccg caccggcgca gccacccacc tccgacgaca
      421 ccctgaataa ctgtacattg ctcaaactac tagcccacta cgcggatcag atagcaggtt
      481 tcaaaacccc cgctctccct cccgtgccac ctggaatcat cggcctgttc acatgcgtgg
      541 aacagatgta ccacgcatgt tttcagaaat actgggcagc tgcactaccc ccaatgtgga
      601 tactgacata cgaccctccc acttctccgt tacaggactg gcttatagtc gcctatggta
      661 acaaggaagg actgctactc ccctctggca taccctcgga ggaggtgtta gccaaaacat
      721 tagtaacaga acaccacgag ttgttcgtat cgcggtcgaa ttcgaccgag accgccgtca
      781 ccatgcccgt atccaaagaa cgcgccctcg ccatctaccg ggtgttcgcc aagggtgagg
      841 tggtggcgga aaatactccc attcttgcct tcaccgacgt ggaactatcc acactcaaac
      901 cccactatct gttcatctat gattttatca tagaggcatt atgcaagagc tacacatact
      961 catgcacgca ggcccgcctg gaatcctttt tgagccgagg tatagacttc atgactgacc
     1021 taggtcagta cctagatacc gctactagcg gcaagcagca gctgacgcac agccaaataa
     1081 aggaaatcaa atacaggctg ctaagctgcg gtctctcggc ttccgcgtgt gatgttttca
     1141 gaactgtgat catgaccctc ccatatcgac cgacccccaa cctcgctaac ctgtccacgt
     1201 ttatggggat ggttcaccaa ctgaccatgt tcggacacta tttctaccgg tgcctgggca
     1261 gctacagtcc caccggcttg gccttcacag aattgcaaaa gatactgaca cgcgccagcg
     1321 cggagcaaac ggaacgtaac ccgtggagac atccgggtat ctcggacatt ccactgcgtt
     1381 ggaaaatatc gcgtgctcta gcattcttcg tcactccggc ccccataaac actttgcagc
     1441 gcgtgtacgc cgcgctgccc tcgcaactca tgcgggccat cttcgagatc tcggtcaaga
     1501 ccacatgggg aggcgccgta ccggcaaacc tggcgcgcga cattgacaca ggaccgaaca
     1561 cacaacatat ctcctccaca ccaccgccca ccctcaagga tgttgagaca tactgtcaag
     1621 gtctgcgggt gggagacacg gagtacgatg aggacattgt gagaagcccg ctctttgcag
     1681 acgcgtttac caagagtcac ttgttgccta tactgcgcga ggttctggaa aaccgcctgc
     1741 agaaaaacag agctctgttt cagataagat ggctgataat atttgctgcc gaggcggcaa
     1801 ccgggctcat ccctgccagg cgcccgctag ccagagccta cttccacatc atggacattc
     1861 tggaggagag acattcccaa gacgccctat acaacctttt ggactgtatc caggagctct
     1921 tcacccacat caggcaggct gttccagacg cacagtgtcc gcacgccttt ctacagtccc
     1981 tgttcgtctt tcaattccgc cctttcgtac tcaaacacca gcagggtgta accttgtttc
     2041 tagatggctt gcagacatcc ctccccccgg tgataagtct ggccaacctt ggagacaagc
     2101 tgtgtcgtct cgagttcgag tacgacagcg agggcgactt cgtgcgcgtg ccagttgcac
     2161 cgccagaaca accaccgcac gtacatctgt cgcatttcaa gaagacaata cagaccatcg
     2221 aacaggccac cagggaggcc accgtagcca tgacaacaat cgcaaagcca atataccccg
     2281 cctacatccg gttactgcag cggctagaat atcttaacag actcaaccac cacattctca
     2341 ggattccctt cccacaggac gccctttctg aactccagga aacctacctg gcggcgtttg
     2401 cacggttgac aaaattggca gcggacgcag caaacacttg tagctactcc ctcaccaagt
     2461 actttggagt tttattccaa caccagctgg tccccacggc catcgttaaa aaactgctac
     2521 atttcgacga ggctaaagat accacagaag cctttttaca gagcctggca caacccgtag
     2581 tgcagggaca acggcagggg gcggctggcg ggtcgggtgt cctgacgcag aaagaacttg
     2641 agctcttgaa caaaataaac ccacagttta cagacgctca ggctaacatt cctccatcta
     2701 ttaaacgttc atattcaaat aaatatgacg tccctgaggt ctcagtcgac tgggaaacgt
     2761 actcccggtc tgccttcgag gcaccggacg acgaactccg ttttgtccca ctgacgctgg
     2821 caggcctccg gaaactgttt gtcgaagact acaaagacca tgacggtgat tataaagatc
     2881 atgatatcga ttacaaggat gacgatgaca agtagtaacc cgggcgacgg atggtgatcc
     2941 ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc
     3001 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctcgg
     3061 gcgacggatg gtgatccccc tggcca
//
