LOCUS       sequence-353553-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF71 TAGGED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0343
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             590..655
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gtcgactgga tccggtaccg ccaccatggc cacttacgag gttctctgtg aggtggcgcg
       61 gaaactgggc acggatgaca gggaagtggt attgttcctc ctaaacgtgt tcatacctca
      121 acccacactg gcccaattaa ttggagctct tagagcttta aaggaggagg gcaggttaac
      181 gtttcccctg ttagcggaat gtctgtttcg tgcaggtcgc agagacctct tgcgcgacct
      241 gcttcactta gacccgcgtt ttttagagcg ccacctagcg ggcacaatga gttatttcag
      301 cccttatcag ctcactgttc tccacgtaga cggggagctg tgtgcgaggg atattaggtc
      361 tttgatattt ttaagcaagg acactatagg gtctcgcagc acaccacaga cattcttaca
      421 ctgggtgtac tgtatggaaa acttagacct actgggtccc actgacgtgg atgccctaat
      481 gtcaatgctt agatctttgt caagagtaga cctacagcgc caagtgcaaa ccctaatggg
      541 ccttcacctt tccggcccat cacactccca acactatcgc catacaccag actacaaaga
      601 ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta
      661 actcgag
//
