LOCUS       sequence-353555-        1941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orf 73 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1941)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0349
FEATURES             Location/Qualifiers
     source          1..1941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1785..1850
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1871..1895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatccggtac cgccaccatg gcgcccccgg gaatgcgcct gaggtcggga cggagcaccg
       61 gcgcgccctt aacgagagga agttgtagga aacgaaacag gtctccggaa agatgtgacc
      121 ttggcgatga cctacatcta caaccgcgaa ggaagcatgt cgccgactcc gtcgacggcc
      181 gggaatgtgg accccacacc ttgcctatac caggaagtcc cacagtgttc acatccgggc
      241 tgccagcatt tgtgtctagt cctactttac cggtggctcc cattccttca cccgctcccg
      301 caacaccttt acctccaccg gcactcttac cccccgtaac cacgtcttcc tccccaatcc
      361 ctccatccca tcctgtgtct ccggggacca cggatactca ttctccatct cctgcattgc
      421 cacccacgca gtctccagag tcttctcaaa ggccaccgct ttcaagtcct acaggaaggc
      481 cagactcttc aacacctatg cgtccgccac cctcgcagca gactacacct ccacactcac
      541 ccacgactcc tccacccgag cctccctcca agtcgtcacc agactcttta gctccgtcta
      601 ccctgcgtag cctgagaaaa agaaggctat cgtcccccca aggtccctct acactaaacc
      661 caatatgtca gtcgccccca gtctctcccc ctagatgtga cttcgccaac cgtagtgtgt
      721 accccccatg ggccacagag tccccgatct acgtgggatc atccagcgat ggcgatactc
      781 cgccacgcca accgcctaca tctcccatct ccataggatc atcatccccg tctgagggat
      841 cctggggtga tgacacagcc atgttggtgc tccttgcgga gattgcagaa gaagcatcca
      901 agaatgaaaa agaatgttcc gaaaataatc aggctggcga ggataatggg gacaacgaga
      961 ttagcaagga aagtcaggtt gacaaggatg acaatgacaa taaggatgat gaggaggagc
     1021 aggagacaga cgaggaggac gggggcgatg gaaacaaaac gttgagcatc caaagttcac
     1081 aagagcagga gacggtggaa gagcccataa tcttgcacgg gtcgtcatcc gaggacgaaa
     1141 tggaagtgga ttaccctgtt gttagcacac atgaacaaat tgccagtagc ccaccaggag
     1201 ataatacacc agacgatgac ccacaacctg gcccatctcg cgaataccgc tatgtactca
     1261 gaacatcacc accccacaga cctggagttc gtatgaggcg cgttccagtt acccacccaa
     1321 aaaagccaca tccaagatac caacaaccac cggtccctta cagacagata gatgattgtc
     1381 ctgcgaaagc taggccacaa cacatctttt atagacgctt tttgggaaag gatggaagac
     1441 gagatccaaa gtgtcaatgg aagtttgcag tgattttttg gggcaatgac ccatacggac
     1501 ttaaaaaatt atctcaggcc ttccagtttg gaggagtaaa ggcaggcccc gtgtcctgct
     1561 tgccccaccc tggaccagac cagtcgccca taacttattg tgtatatgtg tattgtcaga
     1621 acaaagacac aagtaagaaa gtacaaatgg cccgcctagc ctgggaagct agtcaccccc
     1681 tggcaggaaa cctacaatct tccatagtta agtttaaaaa gcccctgcca ttaacccagc
     1741 caggggaaaa ccaaggtcct ggggactctc cacaggaaat gacagactac aaagaccatg
     1801 acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag tagtaactcg
     1861 agatatctag acccagcttt cttgtacaaa gtggttcgat ctagaatggc tagcaaagga
     1921 gaagaacttc actgtgagat a
//
