LOCUS       sequence-353556-       10888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0477
FEATURES             Location/Qualifiers
     source          1..10888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3826..4414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3879..3899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4297..4308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4486..4719
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4797..4918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4834..4853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4888..4907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4958..5093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5113..5132)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5114..5132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5142..5159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5142..5158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5151..5173)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5300..5755
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5387..5406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5597..5618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5781..5885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5886..5954,5955..6746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6104..6123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6917..7505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7406..7425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7659..7676
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7793..7814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7829..7834,7835..7852,7853..7859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7867..7883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7872..7894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7891..7907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7891..7907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7926..7946
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7928..7946
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7974..8200
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8201..8381
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8428..8553
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9046..9279
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9464..9508
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9739..9949
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9761..9957
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9808..9943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9989..10591
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9989..10008)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10485..10505
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10634..10751
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ccagcagggc cagcaggggc agatggcaag tggagacagc aatctcaact tcaggatggt
     1261 cgcagagatc cagaacgtag agggtcagaa cctgcaggag caggtttgcc ctgagcccat
     1321 tttcaggttc ttcagagaaa acaaggtgga gattgcaagt gcaataacaa ggccatttcc
     1381 tttccttatg ggcctccgag accgctcctt catctccgag cagatgtatg aacattttca
     1441 agaagctttt agaaacctgg tcccagtgac aagagtgatg tattgtgtac tcagtgaact
     1501 ggagaagaca tttggctggt cacatctgga agcattgttc agcaggatta acctgatggc
     1561 ctatcctgat ttaaacgaga tttacagaag cttccagaat gtatgctatg aacactcacc
     1621 tctccaaatg aataatgtaa acgatttaga agatagaccc agattactac catatggtaa
     1681 acaagagaac agcaatgcct gtcatgaaat ggatgatata gcagtgcctc aggaagcctt
     1741 gagctcctcg ccaaggtgtg agccaggttt ctcttcagag tcttgtgagc agttagctct
     1801 cccaaaggct ggtggaggag atgctgaaga tgcacccagc ctactaccag gtgggggagt
     1861 gtcctgtaaa cttgctatac aaatagatga aggagaatca gaagaaatgc ccaagttact
     1921 gccttatgat acagaagttc tagaaagcaa cgggatgata gatgcggcaa ggacatacag
     1981 cacagcacca ggggagaaac agggagagga ggaaggcagg aacagtccca gaaaaagaaa
     2041 ccaagacaag gagaagtacc aagagagtcc agagggaaga gacaaagaga cctttgatct
     2101 aaaaactccc caagtcacta atgaaggaga accagagaag gggctctgtc tactaccagg
     2161 tgaaggagaa gagggcagtg atgactgttc agaaatgtgt gatggagaag agccccagga
     2221 agcctctagc tccctagcaa gatgtgggtc agtttcttgt ttatctgcag agacctttga
     2281 tctaaagact ccccaagtca ctaatgaagg agaaccagag aaggagctca gtctactacc
     2341 aggtgaagga gaagtgtcta gtgaactaga aaatcaccca atgaatgaag aaggagaatc
     2401 agaagagctt gcttctagcc tgctatatga taatgtacca ggagcggagc aatcagcata
     2461 tgaaaatgag aagtgttcct gtgtcatgtg tttctcagaa gaggtgccag gaagcccaga
     2521 agcaaggacg gaaagtgatc aagcgtgtgg cacaatggat actgtggata ttgcaaacaa
     2581 ctccactttg ggaaaaccca agaggaaaag aagaaaaaag agggggcatg gctggagcag
     2641 aatgagaatg agaaggcagg aaaacagcca acaaaatgat aatagcaaag ccgacggcca
     2701 ggtggtctcc agtgaaaaga aggcgaacgt gaatctgaaa gacctttcca agattagggg
     2761 gagaaagaga ggcaaacctg gaacccgctt cactcagagt gacagagctg cacagaaaag
     2821 agtccgatca agagcttcaa gaaagcacaa agatgaaact gtggatttta aggctccttt
     2881 gcttccagtg acctgtggtg gggtgaaggg aattttacat aagaagaaat tgcagcaagg
     2941 aatcttggtg aagtgtatac agactgagga tggaaaatgg ttcaccccca cggaatttga
     3001 aatcaaagga ggccatgcaa gatcaaagaa ctggaggctg agtgtgcgct gtggcgggtg
     3061 gcccctacga tggctgatgg agaatggatt tctgcctgat cctccaagaa tacgttacag
     3121 gaaaaaaaag agaatactga agtctcaaaa caatagctca gttgaccctt gtatgagaaa
     3181 cctggatgag tgtgaggtgt gccgggacgg aggggagctg ttctgttgcg acacttgttc
     3241 aagagtcttc catgaggact gtcacatccc gcctgtggaa gctgagagga ccccgtggaa
     3301 ttgcatcttc tgcaggatga aggagtctcc gggaagccaa cagtgttgtc aggaatctga
     3361 ggtcctggag aggcagatgt gtcctgagga acagttgaaa tgtgagttcc tcctcttgaa
     3421 agtctattgc tgttctgaga gctccttttt tgccaagatt ccatactatt attatattag
     3481 agaggcgtgt caaggcctga aggagcccat gtggttggat aaaatcaaga aaaggctgaa
     3541 tgagcacggt tacccccaag tggaggggtt tgtacaagac atgcgcctca tcttccagaa
     3601 ccacagggcc tcttacaagt acaaggattt tggccaaatg ggatttagac tggaggctga
     3661 gtttgagaag aatttcaagg aagtgtttgc tattcaggaa acaaatggga acaattagta
     3721 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3781 ggacgagcgt gctcagccgc tactaaacgt acgcgttaag tcgacaatca acctctggat
     3841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4201 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4381 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     4441 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4501 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4561 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4621 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4681 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4741 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4801 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4861 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4921 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4981 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5041 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     5101 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     5161 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5221 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5281 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5641 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5701 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7681 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7741 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7801 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7861 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7921 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7981 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     8041 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     8101 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     8161 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     8221 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8281 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8341 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8401 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8461 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8521 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8581 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8641 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8701 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8761 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8821 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8881 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8941 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9001 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9061 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9121 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9181 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9241 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9301 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9361 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9421 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9481 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9541 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9601 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9661 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     9721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     9781 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9961 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    10021 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    10081 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    10141 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    10201 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    10261 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    10321 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    10381 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    10441 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    10501 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    10561 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    10621 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10681 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10741 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10801 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10861 cttctaggtc ttgaaaggag tgggaatt
//
