LOCUS       sequence-353557-       12097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0545
FEATURES             Location/Qualifiers
     source          1..12097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5035..5623
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5088..5108)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5506..5517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5695..5928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6006..6127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6043..6062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6097..6116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6167..6302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6322..6341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6323..6341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6351..6368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6351..6367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6360..6382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6509..6964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6596..6615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6806..6827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6990..7094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7095..7163,7164..7955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7313..7332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8126..8714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8615..8634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8868..8885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9002..9023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9038..9043,9044..9061,9062..9068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9076..9092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9081..9103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9100..9116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9100..9116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9135..9155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9137..9155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9183..9409
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9410..9590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9637..9762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10255..10488
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10673..10717
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10948..11158
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10970..11166
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11017..11152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11198..11800
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11198..11217)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11694..11714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11843..11960
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gacgcgggcg gcagaaaaca cggccatctt tatgtgtaaa tgttgtaacc tcttctcacc
     1261 aaatcagtcg gaactcctct cccacgtttc agagaagcac atggaagaag gggttaatgt
     1321 tgatgagatt attattcccc ttaggcctct gagtacacct gaacccccca actcaagcaa
     1381 aaccggagat gagtttttgg tcatgaagag gaagagaggc aggcctaagg ggtccacgaa
     1441 gaagtccagc acagaagagg agctggcaga aaacatcgtg agtccgactg aggacagccc
     1501 gctggctccg gaggaaggga acagcctgcc tccaagcagc ttggagtgta gcaagtgctg
     1561 tcggaagttc tccaacacgc gccagctgcg gaagcacatc tgcattatcg tgctgaattt
     1621 gggtgaggag gaaggagaag caggtaacga gtctgacctt gaactagaaa agaagtgtaa
     1681 ggaagatgat cgggaaaaag cctcgaaaag accacggtca cagaaaacag agaaagtcca
     1741 gaagatctca ggaaaggagg ccagacagct ttctggggcg aagaaaccca tcataagtgt
     1801 ggttttaact gcacacgaag caattccagg tgctaccaag attgtgccag tggaggctgg
     1861 gccccctgaa acaggagcta caaattctga gaccacttca gcagacctgg tgcctcggag
     1921 aggctaccag gaatacgcca ttcagcagac accttatgag caaccaatga agtcaagcag
     1981 gctaggtccc actcagctca aaatcttcac ttgtgaatac tgcaacaagg tcttcaagtt
     2041 caagcactcg ctgcaggccc acctgaggat ccacaccaat gaaaagccat acaagtgccc
     2101 ccagtgcagc tatgccagtg ccatcaaggc caacctcaat gtgcacctgc gcaagcacac
     2161 tggagagaag ttcgcctgcg actattgctc gttcacctgc ctgagcaagg gccacctcaa
     2221 ggtgcacatc gagcgagtgc acaagaagat caagcagcac tgccgcttct gcaagaagaa
     2281 gtactctgac gtcaagaacc tcatcaagca catccgagac gcgcatgacc cacaggacaa
     2341 gaaggtcaaa gaggccttgg acgagctctg cctgatgacg agggagggca agcggcagct
     2401 gctctatgac tgccacatct gtgagcgcaa gttcaagaac gagctggacc gtgaccgcca
     2461 tatgctggtc cacggagaca agtggccttt tgcctgtgag ctctgtggcc atggggccac
     2521 caagtaccag gcgctggaac tgcatgtcag gaagcacccc ttcgtgtacg tctgtgccgt
     2581 ctgccgcaag aagttcgtca gctccatcag gctgcgcacc cacatcaaag aggtgcacgg
     2641 ggctgcccag gaggccttgg tcttcaccag ttccatcaac cagagcttct gcctcctgga
     2701 acctggtggg gacatccagc aagaagctct gggggaccag ctacagctgg tggaagagga
     2761 gtttgccctc cagggcgtga atgcactcaa ggaagaggcc tgtcctgggg acactcagct
     2821 ggaggagggc cggaaggagc cggaggcccc tggggaaatg cctgccccag ctgtgcacct
     2881 ggcctccccg caggccgaaa gcacagccct gccaccctgt gagctggaaa ccaccgtggt
     2941 ctcctcctca gacctgcatt ctcaagaggt ggtttcagat gattttttgt tgaaaaatga
     3001 tacctcctcc gcagaggctc atgctgctcc tgagaagccc ccagacatgc agcacagaag
     3061 ctcagtccag acgcaaggtg aagtgatcac actactgctg tccaaggccc agagtgctgg
     3121 gtcagatcag gaaagccatg gcgcccagag ccccctaggg gaagggcaga acatggctgt
     3181 gctttcagct ggtgacccag atcccagcag gtgtctcagg tcaaacccag ctgaggcctc
     3241 agacctcctc cctccagtag ctggtggtgg ggacaccatc acacatcagc ctgactcttg
     3301 caaagctgcc cctgagcacc ggtcaggcat caccgctttc atgaaggtcc tgaacagttt
     3361 acagaagaag caaatgaaca ccagcttgtg tgagcggatc cggaaggttt atggagacct
     3421 ggagtgtgaa tactgtggca aacttttttg gtaccaagtg cattttgata tgcatgtccg
     3481 cacccacacc cgggaacatc tgtattattg ctctcagtgt cattattctt ccatcaccaa
     3541 aaactgcctt aaacgccacg taattcagaa acacagtaac atcttgctga agtgtcccac
     3601 cgatggctgt gactactcaa ctccagataa atataagcta caggcacatc ttaaagttca
     3661 cacagcactg gacaaaagga gttattcttg tcctgtttgt gaaaagtctt tttcagagga
     3721 tcgattgata aagtcacata tcaagaccaa ccatcctgag gtctccatga gcaccatttc
     3781 tgaggttctc gggaggaggg ttcagctgaa agggctaatt ggaaagagag ccatgaaatg
     3841 cccatattgt gacttttatt tcatgaagaa tggctcagac cttcagcgtc atatttgggc
     3901 tcatgaaggt gtgaagccct tcaagtgttc tttgtgtgag tatgcaactc gtagcaagag
     3961 taacctcaag gctcatatga atcgtcacag cactgagaaa acccacctat gtgacatgtg
     4021 tggcaagaaa ttcaaatcaa aagggacact gaaaagtcac aaactccttc acactgcaga
     4081 tgggaagcag tttaagtgca cggtgtgtga ctacacagcg gcccagaagc cacagctgct
     4141 gcggcacatg gaacagcatg tctccttcaa gcctttccgc tgtgcccatt gccattactc
     4201 ctgcaacata tctggctctc tgaagcggca ctacaacagg aagcacccta atgaggagta
     4261 tgccaacgtg ggcaccgggg agctggcagc ggaggtgctc atccagcaag gtggtttgaa
     4321 gtgtcctgtt tgcagctttg tatatggcac caaatgggag ttcaataggc acttgaagaa
     4381 caaacatggc ttgaaggtgg tggaaattga tggagacccc aagtgggaga cagcaacaga
     4441 agctcctgag gagccctcca cccagtatct ccacatcaca gaggccgaag aagacgttca
     4501 agggacacag gcagcggtgg ccgcgctcca ggacctgaga tacacctctg agagtggcga
     4561 ccgactggac cccacggccg tgaacatcct gcagcagatc attgagctgg gcgccgagac
     4621 ccatgacgcc actgcccttg cctcggtggt tgccatggca ccagggacgg tgactgtggt
     4681 taagcaggtc accgaggagg agcccagctc caaccacacg gtcatgatcc aggagacggt
     4741 ccagcaagcg tccgtggagc ttgccgagca gcaccacctg gtggtgtcct ccgacgacgt
     4801 ggagggcatt gagacggtga ctgtctacac gcagggcggg gaggcctcgg agttcatcgt
     4861 ctacgtgcag gaggccatgc agcctgtgga ggagcaggct gtggagcagc cggcccagga
     4921 actctagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     4981 ttgtggaaag gacgatctac gtttcagggt gtagttgaca cgcgttaagt cgacaatcaa
     5041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5401 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     5641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5701 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     5941 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6001 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6121 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6241 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6301 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     6361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6481 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     6541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6601 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6661 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6721 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6781 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6841 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6901 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6961 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8881 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8941 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9001 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9061 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     9121 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     9181 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     9241 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     9301 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     9361 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     9421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9601 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9661 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9721 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9781 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9841 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9901 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9961 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10021 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10081 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10141 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10201 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10261 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10321 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10381 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10441 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10501 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10561 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10621 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10681 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10741 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10801 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10861 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
    10921 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
    10981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    11041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    11101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    11161 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
    11221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
    11281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
    11341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
    11401 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
    11461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
    11521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
    11581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    11641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    11701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    11761 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    11821 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    11881 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    11941 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    12001 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    12061 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
