LOCUS       sequence-353559-        9778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2829
FEATURES             Location/Qualifiers
     source          1..9778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2716..3304
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2769..2789)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3187..3198)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3376..3609
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3687..3808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3724..3743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3778..3797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3848..3983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4003..4022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4004..4022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4032..4049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4032..4048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4041..4063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4190..4645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4277..4296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4487..4508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4671..4775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4776..4844,4845..5636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4994..5013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5807..6395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6296..6315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6549..6566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6683..6704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6719..6724,6725..6742,6743..6749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6757..6773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6762..6784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6781..6797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6781..6797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6816..6836
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6818..6836
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6864..7090
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7091..7271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7318..7443
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7936..8169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8354..8398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8629..8839
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8651..8847
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8698..8833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8879..9481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8879..8898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9375..9395
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9524..9641
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 agatatacaa acaaatgcgg aactgaaaag cactcaggag cagtctgtgc ccgcagaaag
     1261 tgcagcggtt ttgaatgact acagtttaac caaatctcat gaaatggaaa atgtggacag
     1321 tggagaaggc ccagccaatg aagatgaaga cataggagat gattcaatga aagtgaaaga
     1381 tgaatacagt gaaagagatg agaatgtttt aaagtcagaa cccatgggaa atgcagaaga
     1441 gcctgaaatc ccttacagct attcaagaga atataatgaa tatgaaaaca ttaagttgga
     1501 gagacatgtt gtctcattcg atagtagcag gccaaccagt ggaaagatga actgcgatgt
     1561 gtgtggatta tcctgcatca gcttcaatgt cttaatggtt cataagcgaa gccatactgg
     1621 tgaacgccca ttccagtgta atcagtgtgg ggcatctttt actcagaaag gtaacctcct
     1681 ccgccacatt aaactgcaca caggggaaaa accttttaag tgtcacctct gcaactatgc
     1741 atgccaaaga agagatgcgc tcacggggca tcttaggaca cattctgtgg agaaacccta
     1801 caaatgtgag ttttgtggaa ggagttacaa gcagagaagt tcccttgagg agcacaagga
     1861 gcgctgccgt acatttcttc agagcactga cccaggggac actggtgaga agcgccactg
     1921 ctttgatgtc aactataatt caagttacat gtatgagaaa gagagtgagc tcatacagac
     1981 ccgcatgatg gaccaagcca tcaataacgc catcagctat cttggcgccg aagccctgcg
     2041 ccccttggtc cagacaccgc ctgctcccac ctcggagatg gttccagtta tcagcagcat
     2101 gtatcccata gccctcaccc gggctgagat gtcaaacggt gcccctcaag agctggaaaa
     2161 gaaaagcatc caccttccag agaagagcgt gccttctgag agaggcctct ctcccaacaa
     2221 tagtggccac gactccacgg acactgacag caaccatgaa gaacgccaga atcacatcta
     2281 tcagcaaaat cacatggtcc tgtctcgggc ccgcaatggg atgccacttc tgaaggaggt
     2341 tccccgctct tacgaactcc tcaagccccc gcccatctgc ccaagagact ccgtcaaagt
     2401 gatcaacaag gaaggggagg tgatggatgt gtatcggtgt gaccactgcc gcgtcctctt
     2461 cctggactat gtgatgttca cgattcacat gggctgccac ggcttccgtg accctttcga
     2521 gtgtaacatg tgtggatatc gaagccatga tcggtatgag ttctcgtctc acatagccag
     2581 aggagaacac agagccctgc tgaagtagta atgaactagt ccgtaacttg aaagtatttc
     2641 gatttcttgg ctttatatat cttgtggaaa ggacgacttt agtgaatgta aggtgagtgc
     2701 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2761 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2821 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2881 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2941 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3001 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3061 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3121 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3181 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3241 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3301 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3361 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3421 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3601 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3661 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3781 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3901 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3961 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4021 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4081 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4141 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4621 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5281 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5941 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6601 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6781 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6841 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6901 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6961 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7021 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7081 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7261 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7321 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7381 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7441 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7501 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7561 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7621 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7681 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7741 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7801 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7861 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7921 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7981 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8041 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8101 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8161 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8221 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8281 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8341 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8401 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8461 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8521 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8581 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8641 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8701 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8761 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8821 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     8881 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8941 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9001 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9061 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9121 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9181 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9241 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9301 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9361 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9421 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     9481 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9541 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9601 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9661 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     9721 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
