LOCUS       sequence-353560-       11752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2566
FEATURES             Location/Qualifiers
     source          1..11752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4690..5278
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4743..4763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5161..5172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5350..5583
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5661..5782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5698..5717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5752..5771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5822..5957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5977..5996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5978..5996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6006..6023)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6006..6022)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6015..6037)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6164..6619
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6251..6270)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6461..6482
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6645..6749
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6750..6818,6819..7610)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6968..6987)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7781..8369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8270..8289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8523..8540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8657..8678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8693..8698,8699..8716,8717..8723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8731..8747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8736..8758
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8755..8771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8755..8771
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8790..8810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8792..8810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8838..9064
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9065..9245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9292..9417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9910..10143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10328..10372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10603..10813
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10625..10821
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10672..10807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10853..11455
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10853..10872)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11349..11369
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11498..11615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ggaggaagag agcgaccaag aggccgaacg cctcggagaa gagcttgtgg ccattgtgga
     1261 gtccccgctg ggccctgtgg ggcttagagc tgcgggcgac ggcagaggcg gcgctggcag
     1321 cggcaactgc ggcggcggcg tcggaatcag cagtcgggat tactgccgac gcttctgtca
     1381 ggtggttgaa gattatgctg gaagatggca ggtccctttg ccacagcttc aggttcttca
     1441 gactgccctt tgttgtttta caacagccag tgcatcattc ccagatgaat gtgagcatgt
     1501 acaatatgtt ttgagtagcc ttgctgtgag tttctttgag ttgctgctgt tctttggaag
     1561 agatgagttt tatgaagagc ccttaaagga tattcttgga tcattccagg aatgccagaa
     1621 tcacctccgc agatatggaa atgtgaatct ggaactggtg actcgaatca ttagagatgg
     1681 tggcccatgg gaagatccag tgttgcaagc tgtccttaaa gctcagccag catctcagga
     1741 gatagtgaac aaatatttaa gttctgaaaa tccactgttc tttgaactac gtgccagata
     1801 cctaattgct tgtgaacgca tacccgaagc aatggctctt attaaatctt gtataaatca
     1861 cccagaaatc agtaaagact tatacttcca tcaagcactc ttcacatgtc tgtttatgtc
     1921 acctgtagaa gatcagctat tccgggagca tttattgaaa actgattgta agagtggaat
     1981 tgatatcatc tgtaatgctg aaaaagaagg caaaactatg ttagccttgc aactctgtga
     2041 atcctttctt attccacagc tccagaatgg ggatatgtac tgtatctggg agttgatttt
     2101 catatggagt aaactacagc ttaaatctaa tccttcaaaa caagtttttg tagatcaatg
     2161 ctaccagctt ttaagaacag caactaatgt gagagtcata tttcctttca tgaaaatcat
     2221 caaagatgag gttgaagaag aaggcttgca aatttgtgtt gaaatatgtg gttgtgctct
     2281 acaactcgac cttcatgatg atcccaaaac taaatgtcta atttataaaa caattgcaca
     2341 ttttttgcca aatgatttgg agatcctcag gatttgtgca ctctcaatat tttttctgga
     2401 gcgctcctta gaagcgtatc gtactgttga agagctttac aaacgtccag atgaagaata
     2461 taatgaaggc acaagtagtg ttcaaaatcg tgttcgtttt gaattgcttc caattttgaa
     2521 aaagggattg ttttttgacc ctgaattttg gaacttcgta atgattaaga aaaactgtgt
     2581 agcattattg agtgataaat cagcagttag atttctaaat gaaagcacac tggaaaataa
     2641 tgcaggtaat ctaaaaagga cggaggaaca gcaaggtttg gatgaagggt ttgactctct
     2701 tacagatcag agcactggag agactgatcc tgatgatgta tctggagtgc agcctaaagg
     2761 tcatattaat acgaagaaaa atcttacagc tctcagtact tccaaagtag atcacaatgt
     2821 cccaaggcat cgttgtatgt tatgtaacaa ggaattttta ggtggtcaca ttgtaaggca
     2881 tgcccaggct catcagaaaa aaggcagttt tgcatgtgta atatgtggta ggaaatttag
     2941 aaacagagga cttatgcaga agcatttgaa gaatcatgtt aagaagatac agaggcagca
     3001 aattgctgca gctcaacagg atgatcagga agtcactgct ttggaagaaa taaattgttc
     3061 tagttcttcc atttcatttg aaaatgggaa ttctgatagt aaggatttgg aagtggagac
     3121 acttactgct tctagtgaag gaaacaaaga agtcatccct gagcatgtgg ctgaattcat
     3181 tgaaattccc ataagtgtac cagaagatgt tattgaaaat gttattgaaa atggcagtcc
     3241 taataattct ttaaataatg ttttcaagcc tttaactgaa tgtggggatg attatgagga
     3301 ggaagaggat gaagaaggtg attatgaaga agatgattat gacctgaatc aagaaacttc
     3361 agtaattcat aaaatcaatg gaactgtgtg ccatccaaaa gacatatatg ccacagatca
     3421 agaaggaaac tttaagtgtc ctgctcttgg ttgtgtccgg atatttaaaa gaattgggtt
     3481 tctaaataaa catgcaatga ccgtacatcc aaccgattta aatgtgcgac aaacagtaat
     3541 gaagtggagc aaaggaaaat gcaaattttg tcaaaggcaa tttgaagatt ctcaacattt
     3601 tatagaccac cttaatagac atagctatcc aaatgtgtat ttttgtttgc attttaattg
     3661 caacgagtcg tttaagctgc cgttccagct tgcccagcac acaaaaagtc acaggatatt
     3721 tcaggctcag tgtagttttc cagaatgcca tgagcttttt gaagatcttc ctctgctgta
     3781 tgaacatgaa gctcaacact atttaagtaa aacaccagag tcatctgcac aaccaagtga
     3841 aacaattctt tgggatgttc agacagactc aaatcctaat caggaaaaag actcatctag
     3901 taatgagaaa caaactatta gtctgccagt ttctactagc aaatcaagga aagagtctac
     3961 agaaccaaag acatgtatag aaagtatgga aaagaaaaca gacagtttag ttcagaatgg
     4021 aaacgaacgt tctgatgaca ctgtttcaaa tataagcttg atagaccaaa agatgcctga
     4081 catagagcca aattctgaaa ataattgtag tagtagtgat atagtcaatg gacacagtga
     4141 aatagagcaa acacctttag tttcatcaga tcctgctttg aaaattgata caaacagaat
     4201 caggacagaa aatggttcca ttttgcccag tgttgtacca caagaacaca acaccttgcc
     4261 agtatctcag gcaccttcca aaccaaatct gacaagtgaa catacttcat atggcttaat
     4321 tttaacaaaa ccatacgtca gaccattgcc tcccagttac cttgatgaac ggtatcttag
     4381 tatgccaaaa cgcagaaaat ttctgactga tagagtagat gcctgttctg atcaagataa
     4441 cgtgtataaa aaatcagtga aaagattaag atgtggcaaa tgcctgacca cctactgtaa
     4501 tgcagaagca cttgaggctc atcttgcaca aaagaaatgt cagacactct ttggatttga
     4561 ttcagatgat gaaagtgcct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     4621 ttggctttat atatcttgtg gaaaggacga ggaaacatgc cggtttagaa cgctacgcgt
     4681 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4741 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4801 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4861 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4921 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4981 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5041 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5101 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5161 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5221 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     5281 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5341 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5401 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5461 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5521 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5581 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5641 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5701 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5761 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5881 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5941 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6001 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6061 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6121 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6181 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6241 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6301 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6361 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6421 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6481 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6541 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6601 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6661 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6721 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6781 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6841 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6901 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6961 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7021 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7081 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7141 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7201 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7261 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7321 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7381 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7441 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7501 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7561 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7621 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7681 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7741 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7801 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7861 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7921 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7981 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8041 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8101 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8161 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8221 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8281 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8341 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8401 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8461 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8521 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8581 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8641 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8701 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8761 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8821 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8881 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8941 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9001 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9061 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9241 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9301 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9361 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9421 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9481 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9541 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9601 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9661 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9721 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9781 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9841 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9901 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9961 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10021 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10081 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10141 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10201 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10261 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10321 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10381 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10441 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10501 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
    10561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10621 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10741 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10801 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
    10861 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10921 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10981 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    11041 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    11101 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    11161 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    11221 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    11281 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    11341 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    11401 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    11461 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    11521 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    11581 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    11641 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    11701 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
