LOCUS       sequence-353565-       10255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3208
FEATURES             Location/Qualifiers
     source          1..10255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3009..3033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3041..3082
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3193..3781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3246..3266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3664..3675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3853..4086
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4164..4285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4201..4220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4255..4274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4325..4460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4480..4499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4481..4499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4509..4526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4509..4525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4518..4540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4667..5122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4754..4773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4964..4985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5148..5252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5253..5321,5322..6113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5471..5490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6284..6872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6773..6792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7026..7043
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7160..7181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7196..7201,7202..7219,7220..7226)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7234..7250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7239..7261
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7258..7274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7258..7274
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7293..7313
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7295..7313
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7341..7567
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7568..7748
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7795..7920
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8413..8646
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8831..8875
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9106..9316
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9128..9324
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9175..9310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9356..9958
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9356..9375)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9852..9872
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10001..10118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcagc tctgtccccc acatttgcca cgtccaccca
     1261 ggattccacc tgtctccagg actcagaatt tcctgtttct tcaaaagacc attcctgtcc
     1321 ccagaatttg gacctgttcg tgtgcagtgg tctggagcct cacacaccca gtgttggttc
     1381 ccaggaatcg gtgactttcc aagatgtcgc tgtggacttt accgagaagg agtggccctt
     1441 gctggattct tctcagagaa aactgtacaa agatgtaatg ctggagaact acagcaacct
     1501 cacttcactg gggtatcagg tcggcaaacc cagcctcatc tcacacttgg agcaggagga
     1561 ggagccgagg acagaggaga ggggcgctca ccagggcgct tgtgcagatt gggagactcc
     1621 atctaaaacc aagtggtcac ttcttatgga agatattttt gggaaggaaa cgcccagtgg
     1681 tgtgacgatg gaaagagccg gtcttggaga gaagtccact gaatacgctc acttgttcga
     1741 agtctttggc atggaccctc atctcactca gccaatggga aggcacgctg gcaagaggcc
     1801 ctatcaccgc cgcgactatg gggtagcgtt caagggcagg ccgcacctca ctcagcacat
     1861 gagcatgtac gacgggagaa aaatgcatga atgtcatcag tgccaaaaag ccttcaccac
     1921 gagcgcgtcc ctcacacggc acaggagaat ccacaccggg gagaagcctt acgagtgcag
     1981 cgactgcggg aaagccttca acgacccttc agcccttagg agccacgcaa gaactcacct
     2041 caaagagaag ccctttgact gcagtcagtg tggaaatgca ttccggaccc tctcggccct
     2101 gaaaatccac atgcgagttc acactggcga gaggccttac aagtgtgatc agtgcgggaa
     2161 ggcttacggc cggagctgcc acctcatcgc acacaagaga acgcacaccg gagagaggcc
     2221 ctacgagtgt cacgactgtg ggaaagcttt ccagcacccc tcccacctca aagagcacgt
     2281 gaggaatcac acgggggaga agccctacgc gtgcacgcag tgcggcaaag ccttccgctg
     2341 gaagtccaac tttaatttgc acaagaagaa ccacatggtg gagaagacct acgaatgtaa
     2401 agaatgcggg aaatcctttg gcgatctcgt gtcccggagg aaacacatga ggattcacat
     2461 cgtcaagaaa cccgtggaat gtcggcagtg cgggaagacc ttccgaaacc agtccatcct
     2521 taagactcac atgaactctc acactggaga gaaaccatac gggtgcgatc tctgcgggaa
     2581 agctttcagc gcgagttcaa acctcaccgc acacaggaag atacacacgc aagagagacg
     2641 ctacgaatgc gccgcctgcg ggaaagtctt cggtgactat ttatcccggc ggaggcacat
     2701 gagcgttcac cttgtaaaga aacgagttga gtgtaggcag tgtggcaagg ccttcaggaa
     2761 ccagtcaacg ctgaagacgc acatgcgaag ccacacgggg gagaaaccgt acgaatgcga
     2821 tcactgtggg aaggccttca gcataggctc caacctgaat gtgcacaggc ggatccacac
     2881 cggggagaag ccctacgaat gccttgtctg cgggaaagcc ttcagcgacc actcatccct
     2941 caggagccac gtgaaaactc accggggaga gaagctcttt gtgtcatccg tgtggaaaag
     3001 gctccagtgc ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     3061 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     3121 ttcttggctt tatatatctt gtggaaagga cgaagtaaaa aacagcttat aaacctcacg
     3181 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3241 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3301 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3361 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3421 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3481 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3541 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3601 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3661 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3721 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3781 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3841 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3901 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3961 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4021 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4081 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4141 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4261 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4381 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4441 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4501 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4561 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4621 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4681 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4741 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4801 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4861 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4921 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4981 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5041 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5101 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5461 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5641 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5761 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6961 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7021 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7081 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7141 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7201 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7261 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7321 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7381 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7441 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7501 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7561 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7621 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7681 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7741 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7801 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7861 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7921 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7981 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8041 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8101 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8161 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8221 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8281 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8341 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8401 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8461 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8521 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8581 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8641 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8701 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8761 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8821 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8881 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8941 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9001 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     9061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9121 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9301 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     9361 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9421 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9481 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9541 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     9601 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9661 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9721 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9781 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9841 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9901 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     9961 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10021 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10081 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10141 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    10201 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
