LOCUS       sequence-353568-       10351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0156
FEATURES             Location/Qualifiers
     source          1..10351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3289..3877
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3342..3362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3760..3771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3949..4182
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4260..4381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4297..4316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4351..4370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4421..4556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4576..4595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4577..4595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4605..4622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4605..4621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4614..4636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4763..5218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4850..4869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5060..5081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5244..5348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5349..5417,5418..6209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5567..5586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6380..6968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6869..6888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7122..7139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7256..7277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7292..7297,7298..7315,7316..7322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7330..7346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7335..7357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7354..7370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7354..7370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7389..7409
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7391..7409
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7437..7663
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7664..7844
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7891..8016
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8509..8742
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8927..8971
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9202..9412
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9224..9420
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9271..9406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9452..10054
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9452..9471)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9948..9968
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10097..10214
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gtccacggat tacagtacct atagccaagc tgcagcgcag cagggctaca gtgcttacac
     1261 cgcccagccc actcaaggat atgcacagac cacccaggca tatgggcaac aaagctatgg
     1321 aacctatgga cagcccactg atgtcagcta tacccaggct cagaccactg caacctatgg
     1381 gcagaccgcc tatgcaactt cttatggaca gcctcccact gtagaaggga ccagtacagg
     1441 ttatactact ccaactgccc cccaggcata cagccagcct gtccaggggt atggcactgg
     1501 tgcttatgat accaccactg ctacagtcac caccacccag gcctcctatg cagctcagtc
     1561 tgcatatggc actcagcctg cttatccagc ctatgggcag cagccagcag ccactgcacc
     1621 tacaagaccg caggatggaa acaagcccac tgagactagt caacctcaat ctagcacagg
     1681 gggttacaac cagcccagcc taggatatgg acagagtaac tacagttatc cccaggtacc
     1741 tgggagctac cccatgcagc cagtcactgc acctccatcc taccctccta ccagctattc
     1801 ctctacacag ccgactagtt atgatcagag cagttactct cagcagaaca cctatgggca
     1861 accgagcagc tatggacagc agagtagcta tggtcaacaa agcagctatg ggcagcagcc
     1921 tcccactagt tacccacccc aaactggatc ctacagccaa gctccaagtc aatatagcca
     1981 acagagcagc agctacgggc agcagagttc attccgacag gaccacccca gtagcatggg
     2041 tgtttatggg caggagtctg gaggattttc cggaccagga gagaaccgga gcatgagtgg
     2101 ccctgataac cggggcaggg gaagaggggg atttgatcgt ggaggcatga gcagaggtgg
     2161 gcggggagga ggacgcggtg gaatgggcgc tggagagcga ggtggcttca ataagcctgg
     2221 tggacccatg gatgaaggac cagatcttga tctaggccca cctgtagatc cagatgaaga
     2281 ctctgacaac agtgcaattt atgtacaagg attaaatgac agtgtgactc tagatgatct
     2341 ggcagacttc tttaagcagt gtggggttgt taagatgaac aagagaactg ggcaacccat
     2401 gatccacatc tacctggaca aggaaacagg aaagcccaaa ggcgatgcca cagtgtccta
     2461 tgaagaccca cccactgcca aggctgccgt ggaatggttt gatgggaaag attttcaagg
     2521 gagcaaactt aaagtctccc ttgctcggaa gaagcctcca atgaacagta tgcggggtgg
     2581 tctgccaccc cgtgagggca gaggcatgcc accaccactc cgtggaggtc caggaggccc
     2641 aggaggtcct gggggaccca tgggtcgcat gggaggccgt ggaggagata gaggaggctt
     2701 ccctccaaga ggaccccggg gttcccgagg gaacccctct ggaggaggaa acgtccagca
     2761 ccgagctgga gactggcagt gtcccaatcc gggttgtgga aaccagaact tcgcctggag
     2821 aacagagtgc aaccagtgta aggccccaaa gcctgaaggc ttcctcccgc caccctttcc
     2881 gcccccgggt ggtgatcgtg gcagaggtgg ccctggtggc atgcggggag gaagaggtgg
     2941 cctcatggat cgtggtggtc ccggtggaat gttcagaggt ggccgtggtg gagacagagg
     3001 tggcttccgt ggtggccggg gcatggaccg aggtggcttt ggtggaggaa gacgaggtgg
     3061 ccctgggggg ccccctggac ctttgatgga acagatggga ggaagaagag gaggacgtgg
     3121 aggacctgga aaaatggata aaggcgagca ccgtcaggag cgcagagatc ggccctacta
     3181 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     3241 aaaggacgaa gaggtgaata ctttgatcat tccacgcgtt aagtcgacaa tcaacctctg
     3301 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3361 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3421 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3481 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3541 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3601 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3661 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3721 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3781 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3841 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     3901 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3961 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4021 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4081 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4141 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     4201 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4381 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4501 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4561 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4741 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5101 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5221 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5281 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5341 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5401 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5461 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5521 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5581 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5641 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5701 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5761 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5821 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5881 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5941 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6001 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6061 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6121 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6181 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6241 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6301 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7261 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7321 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7381 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7441 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7501 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7561 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7621 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7681 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7741 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7801 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7861 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7921 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7981 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8041 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8101 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8161 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8221 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8281 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8341 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8401 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8461 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8521 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8581 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8641 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8701 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8761 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8821 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8881 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8941 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9001 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9061 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9121 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     9181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     9241 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9421 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     9481 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9541 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9601 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9661 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9721 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9781 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9841 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9901 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9961 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10021 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
    10081 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    10141 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    10201 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
    10261 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
    10321 gaccttctag gtcttgaaag gagtgggaat t
//
