LOCUS       sequence-353571-       12826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0886
FEATURES             Location/Qualifiers
     source          1..12826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5764..6352
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5817..5837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6235..6246)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6424..6657
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6735..6856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6772..6791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6826..6845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6896..7031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7051..7070)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7052..7070)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7080..7097)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7080..7096)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7089..7111)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7238..7693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7325..7344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7535..7556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7719..7823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7824..7892,7893..8684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8042..8061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8855..9443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9344..9363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9597..9614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9731..9752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9767..9772,9773..9790,9791..9797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9805..9821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9810..9832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9829..9845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9829..9845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9864..9884
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9866..9884
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9912..10138
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10139..10319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10366..10491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10984..11217
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11402..11446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11677..11887
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11699..11895
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11746..11881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11927..12529
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11927..11946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12423..12443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12572..12689
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cgccaatgtg ggatcgatgt ttcaatattg gaagcgattt gatctacggc gactccagaa
     1261 ggagcttaat tccgtcgctt ctgagctgtc tgcacggcag gaggagagtg aacattctca
     1321 taaacattta attgaactcc gccgggaatt taagaaaaat gtacctgagg aaatcagaga
     1381 gatggtggct cctgtattaa aaagcttcca agccgaggtg gtggccctta gtaagagaag
     1441 tcaggaggcg gaggctgctt ttctgagtgt ttacaagcaa ttaattgaag caccagaccc
     1501 cgtgcctgtg tttgaggcgg cacgcagcct agacgacaga ctgcagcccc ccagctttga
     1561 ccccagtggg cagccccggc gagacctcca cacttcgtgg aagaggaacc ccgagctcct
     1621 cagccccaaa gagcagagag aggggacgtc gcctgccggg cccacgctga ccgagggaag
     1681 ccgcctccca ggcattcccg ggaaagccct cctgacagaa accttgctgc agagaaatga
     1741 ggcggaaaaa caaaagggcc ttcaagaagt acagatcact ttggcggcca gactggggga
     1801 ggcagaggag aaaatcaaag tcctacattc agcgctaaag gctacgcagg cagagctgct
     1861 agagctgcgg cggaagtacg acgaggaggc agcatccaag gcagatgaag tcggcctgat
     1921 catgaccaac ctggagaaag ctaatcagcg agctgaggct gcccagcggg aggtggaaag
     1981 tctccgggaa cagctggcct ctgtcaacag ctccatccgc ctggcttgct gctctcccca
     2041 ggggcccagt ggggataagg tgaacttcac tctgtgctcg ggccctcggc tggaggccgc
     2101 gctggcctcc aaggacaggg agatcctgcg gctgctgaag gacgtgcagc acctccagag
     2161 ctcactgcag gagctggagg aggcatccgc caaccagatc gccgacctgg agcggcagct
     2221 cacggccaag tccgaggcca tagaaaagct ggaagagaag ctccaggccc agtctgacta
     2281 tgaggaaatt aaaacggagc tgagcatcct gaaagccatg aagctggcct ccagcacctg
     2341 cagcctcccc cagggcatgg ccaagcctga agactcactg cttattgcaa aggaggcctt
     2401 cttccccacg cagaaattcc ttctggagaa gcccagcctc ctggccagcc ctgaggaaga
     2461 cccatcagag gacgattcca tcaaggattc actgggcacg gagcagtcct acccctcccc
     2521 tcagcagctc ccacctccac cagggccaga agaccccctg tctcccagcc ccgggcagcc
     2581 cctgctgggc cccagcttgg ggcctgacgg cactcggact ttctcgctgt cccccttccc
     2641 cagcctggca tcaggggaga gactgatgat gcccccagcc gccttcaagg gagaggcggg
     2701 cggcctgctg gtgttccccc cagccttcta tggcgccaag ccccccacag cccctgccac
     2761 cccggcccct ggccctgagc cactgggcgg tcctgagccc gcggatggtg gtgggggcgg
     2821 agcggcgggg cccggggcag aggaggagca gctggacacg gcagagatcg ccttccaggt
     2881 gaaggagcag ctgctgaaac acaacatcgg gcagcgggtg tttgggcatt acgtgctggg
     2941 gctgtcgcag ggctcggtca gcgagatcct agcccggccc aagccctggc gcaagctcac
     3001 ggtgaagggc aaggagccct tcatcaagat gaagcagttc ctgtcggatg agcagaatgt
     3061 actggcgctc aggaccatcc aagtgcggca gcgaggcagc atcaccccga gaatccgcac
     3121 gcctgagaca ggctcagacg acgccatcaa gagcattcta gagcaggcca agaaggagat
     3181 cgagtcgcag aagggcggcg agcccaagac ctcggtggcc ccgctgagca tcgccaacgg
     3241 cacgaccccc gccagcacct cggaggacgc catcaagagc atcctggagc aggcacgccg
     3301 tgagatgcag gcgcaacagc aggcgctgct ggagatggag gtggcgccca ggggccgctc
     3361 ggtgcccccc tcgcccccgg agcggccatc actggccacc gcgagccaga acggggcccc
     3421 ggccttggtg aagcaggagg agggcagcgg gggccccgcg caggcgccgc tcccggtcct
     3481 gtcccccgcc gccttcgtgc agagcatcat ccgcaaggtc aagtccgaga tcggcgacgc
     3541 cggctacttc gaccaccact gggcctccga ccgcggcctg ctcagccgcc cctacgcctc
     3601 cgtgtcgccc tcgctgtcct cctcctcctc ctctggctac tctggccagc ccaacggccg
     3661 cgcctggccc cgcggggacg aggcccctgt gccccccgag gacgaggcgg cggcaggggc
     3721 ggaggacgaa ccccccagga cgggcgagct caaggctgag ggcgcgacgg ccgaggcggg
     3781 cgcgcggctg ccctactacc cggcctacgt gccgcgcacc ctgaagccca ccgtgccgcc
     3841 gctgaccccc gagcagtacg agctgtacat gtaccgtgag gtagacacgc tggagctcac
     3901 ccgccaggtc aaggagaagc tggccaagaa cggcatctgc cagaggatct tcggggagaa
     3961 ggtgctgggc ctgtcacagg gcagcgtgag cgacatgctg tcccggccga agccatggag
     4021 caagctgacg cagaaggggc gggagccctt catccgcatg cagctgtggc tctctgacca
     4081 gctcggccag gcagtgggcc agcagcctgg tgcctcccag gccagtccca cagaaccaag
     4141 gtcctcacca tccccacccc ccagccccac agagcctgag aagagctccc aggagccgtt
     4201 gagcctgtcc ctggagagca gcaaggagaa ccagcagcca gagggccgct ccagctcctc
     4261 gttgagcggg aagatgtact caggcagcca ggccccaggg ggcatccagg agatcgtggc
     4321 catgtccccc gagctggaca cgtactccat caccaagagg gtgaaggagg tcctcacaga
     4381 caacaatcta gggcagcggc tgtttgggga aagcatcctg ggtctgacac agggctccgt
     4441 gtctgacctg ctgtcccggc ccaaaccctg gcacaagctg agcctgaagg ggcgggagcc
     4501 ttttgtccgc atgcagctgt ggctcaatga cccccataac gtggagaagc tgagggatat
     4561 gaagaagctg gagaagaaag cctacctgaa acgtcgctat ggcctcatca gcaccggctc
     4621 agacagtgag tccccggcca cccgctcaga gtgccccagc ccctgcctgc agccccagga
     4681 cctgagcctc ctgcagatca agaagccccg ggtggtgctg gcacccgagg agaaggaggc
     4741 actgcggaag gcctatcagc tggaacccta cccctcgcag cagaccatcg agctcctctc
     4801 cttccagctc aacctcaaga ccaacaccgt catcaactgg ttccacaact acaggtcccg
     4861 gatgcgccgg gagatgttgg tggaggggac ccaggatgag ccagaccttg atccaagcgg
     4921 gggtcctgga atcctaccgc caggccactc ccacccagac cccaccccgc agagccctga
     4981 ctctgagact gaggaccaga agccaaccgt gaaggaactg gagcttcagg agggccctga
     5041 ggagaacagc acacccctga ccacccagga caaggcccaa gtgaggatca agcaggaaca
     5101 gatggaggag gatgctgagg aagaggcagg cagccagccc caggactcag gggagctgga
     5161 caaaggccaa ggtcccccca aagaggagca tcccgaccct ccgggtaatg atggactccc
     5221 aaaagtggct cccgggcccc tccttccagg tggatccacc ccagactgtc cctcacttca
     5281 tccccaacag gagagtgagg ccggggagcg acttcacccg gaccctttaa gttttaagtc
     5341 agcctcagag tcctcacgct gcagcctgga ggtgtcactg aactcgccct cggccgcctc
     5401 ctcaccaggc ctcatgatgt ctgtgtcacc tgtcccctcc tcctcagctc ccatctcccc
     5461 atccccacct ggcgcccccc ctgccaaagt gccgagtgcc agccccactg ctgacatggc
     5521 tggagccttg caccccagtg ccaaggtgaa ccccaacttg cagcggcggc atgagaagat
     5581 ggccaatctg aacaacatca tttaccgagt agagcgggct gccaatcggg aggaggccct
     5641 ggagtgggag ttctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     5701 ttatatatct tgtggaaagg acgactagga ctagagaggt cgcggaaaac gcgttaagtc
     5761 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5821 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5881 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5941 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6001 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6061 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6121 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6181 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6241 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6301 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     6361 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6421 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6481 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6541 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6601 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6661 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6721 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6841 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6961 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7021 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7081 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7141 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7201 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7261 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7321 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7381 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7441 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7501 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7561 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7681 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7801 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7861 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7921 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7981 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8041 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8101 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8161 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8221 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8281 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8341 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8401 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8461 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8521 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8581 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8641 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8701 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8761 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8821 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8881 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8941 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9001 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9061 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9541 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9601 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9661 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9721 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9781 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9841 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9901 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     9961 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
    10021 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
    10081 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
    10141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10321 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    10381 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    10441 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    10501 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    10561 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    10621 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    10681 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    10741 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    10801 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    10861 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10921 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10981 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11041 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11101 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11161 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    11221 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    11281 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    11341 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    11401 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    11461 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    11521 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    11581 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
    11641 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    11701 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    11761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    11821 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11881 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
    11941 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
    12001 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
    12061 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    12121 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
    12181 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    12241 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    12301 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    12361 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    12421 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    12481 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
    12541 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    12601 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    12661 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    12721 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
    12781 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
