LOCUS       sequence-353576-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hA8
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(542..547,548..564,565..570))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(697..1285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(777..796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1558..1574
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1558..1574
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1579..1598
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1579..1597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1772..2468,1..494))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gcaaggaatg gtgcatgtaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
       61 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
      121 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
      181 acgatgcgtc cggcgtagag aatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
      241 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
      301 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
      361 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
      421 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
      481 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
      541 cattaaagct aatcgatgat aagctgtcaa acatgagaat taattcatgt tctttcctgc
      601 gttatcccct gattctgtgg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1321 tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgaccagat
     1381 atggtctcac gcggccaacg ccggatcagg gcgagataac tgggcaacaa tgctctccag
     1441 cgccggcctg ccgccaatat tacttcgaga ccagctttgg tctagaggag gcatgatgat
     1501 atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgccag
     1561 gaaacagcta tgaccatgta atacgactca ctatagggga tattttattt tctcccaatc
     1621 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatcaaggg
     1681 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     1741 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     1801 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     1861 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg
     1921 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     1981 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     2041 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     2101 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     2161 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     2221 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     2281 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     2341 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatg ggtcggcgct
     2401 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     2461 ccgccgcc
//
