LOCUS       sequence-353589-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AT2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     373..389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     373..389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        394..412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(587..1777)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1406..1425
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1825..1830,1831..1847,1848..1853))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1980..2568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2060..2079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct cgaccagata
       61 tggtctcgca ggttgtagcg attgctagta atattggtgg caaacaggct cttgagacgg
      121 ttcagcgcct ccttccagtt ctctgtcaag cccacggact caccccagat caagttgtag
      181 cgattgctag taatgggggt ggcaaacagg ctcttgagac tgttcagcgc cttctaccag
      241 ttctctgtca agcccacggc ctgacgcccg agcaagtggt tgagaccagc tttggtctag
      301 aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
      361 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatattt
      421 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
      481 ttccccgatc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
      541 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
      601 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
      661 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
      721 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
      781 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
      841 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
      901 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
      961 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     1021 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     1081 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     1141 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     1201 gcatgggtcg gcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     1261 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg taaggagatg gcgcccaaca
     1321 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     1381 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     1441 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg
     1501 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     1561 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     1621 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     1681 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     1741 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     1801 aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag
     1861 aattaattca tgttctttcc tgcgttatcc cctgattctg tggataacgc aggaaagaac
     1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2581 atcttttcta cggggtctga cgct
//
