LOCUS       sequence-353598-        8444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red-shifted luciferase under ScPGK1 promoter in Candida 
            glabrata.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Schrevens S, Sanglard D
  TITLE     Investigating Candida glabrata Urinary Tract Infections (UTIs) in 
            Mice Using Bioluminescence Imaging.
  JOURNAL   J Fungi (Basel). 2021 Oct 9;7(10). pii: jof7100844. doi: 
            10.3390/jof7100844.
  PUBMED    34682265
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2021-10-9"; volume: "7(10). pii: jof7100844. doi";
            pages: " 10.3390/jof7100844"
COMMENT     Sequence Label: pSSa13
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(941..960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1060..1082
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1120..1138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1776..1847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1848..1916,1917..2708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2066..2085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2879..3467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3368..3387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3621..3638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3755..3776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3791..3796,3797..3814,3815..3821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3829..3845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3834..3856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3853..3869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3853..3869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3888..3908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3890..3908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4478..4494
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4478..4494
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6165..6184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6166..6184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6194..6211)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6194..6210)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6203..6225)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6351..6806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6438..6457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6648..6669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtcatactaa cccattttta tagaagaagt tgctgcttta gttatcgata acggttctca
       61 tatgaaaatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
      121 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
      181 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
      241 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
      301 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
      361 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
      421 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
      481 tactctcggc ggcattgacc tcttcacgta taaaactaga cctcaagtct cgaacgaaac
      541 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacata tgtgaagtgt
      601 gaagggggag attttcactt tattagattt gtatatatgt ataataaata aataaataag
      661 ttaaataaat aattagataa gggtggtaat tattactatt tacaatcaaa ggtggtcctg
      721 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
      781 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
      841 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
      901 aaaaatgatg aattgaaaag gtggtatggt gcactctcag tacaatctgc tctgatgccg
      961 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1021 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1081 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1141 tataggttaa tgtcatgata ataatggttt cttagcaggt tagactaaca tgcaaaaagt
     1201 ttatattcat ttttgtagtt attcttgttt tcattaggat gattacacac tgtggatgtg
     1261 gattaatcga tcattgttaa tttagtgtag ctactaccat ttcaaacaaa acgtaaattc
     1321 ttgcagtcgt actggatctg tgaatctatt agtatatatg aattaaagta gcttgtacat
     1381 attattctgt tgaatcatat cgcagagcat tggttgaaat ccaaaatata aaaatgtaat
     1441 atcacaaaaa ataatactaa ttctaacatt aatgggtcag atttttagtg aatacttaaa
     1501 tttataatat cgtctattta agctagcaaa tggaacaaca tttaaagtaa gaacatcata
     1561 tctacatgaa aatgtatatt tcaatctgac taataacgca gagcacatct ttcagtgatg
     1621 tctgtcacat gatcaaaaag aattgtattt aaataattca taataaaagc ttaaaaaatt
     1681 acaataatga aaataaagta aataatgaca tgggtaagag ttccgaaata gaatcttagt
     1741 gtacaaaaca aaaattgcat cattagagat ccccacattc aaatatgtat ccgctcatga
     1801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3661 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3721 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3781 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3841 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     3901 actaaaggga acaaaagctg gagctccata aagcacgtgg cctcttatcg agaaagaaat
     3961 taccgtcgct cgtgatttgt ttgcaaaaag aacaaaactg aaaaaaccca gacacgctcg
     4021 acttcctgtc ttcctattga ttgcagcttc caatttcgtc acacaacaag gtcctagcga
     4081 cggctcacag gttttgtaac aagcaatcga aggttctgga atggcgggaa agggtttagt
     4141 accacatgct atgatgccca ctgtgatctc cagagcaaag ttcgttcgat cgtactgtta
     4201 ctctctccct ttcaaacaga attgtccgaa tcgtgtgaca acaacagcct gttctcacac
     4261 actcttttct tctaaccaag ggggtggttt agtttagtag aacctcgtga aacttacatt
     4321 tacatatata taaacttgca taaattggtc aatgcaagaa atacatattt ggtcttttct
     4381 aattcgtagt ttttcaagtt cttagatgct ttctttttct cttttttaca gatcatcaag
     4441 gaagtaatta tctacttttt acaacaaata taaaacatct agaactagtg gatccaaaat
     4501 ggaggatgct aagaacatta agaagggccc tgcccctttc tatccattag aagatggtac
     4561 agcaggtgaa caacttcaca aagctatgaa gagatacgct ttagtcccag gtactattgc
     4621 cttcactgat gcccatatag aggttgacat aacatacgcg gagtacttcg agatgtcagt
     4681 gagattagcg gaagccatga agagatatgg cttgaatact aatcacagga ttgtggtttg
     4741 ctctgaaaac tcattgcagt tctttatgcc agttttgggt gcattattca ttggtgtcgc
     4801 tgttgctcca gcaaatgata tatacaacga aagagaatta ttgaactcta tggggatctc
     4861 tcaaccaact gtcgtgtttg tcagcaagaa gggattgcaa aagattctta acgttcagaa
     4921 gaaacttccc atcattcaga agatcatcat catggacagc aaaaccgact atcaaggctt
     4981 ccaatcaatg tatacctttg ttacatctca cctaccacct gggtttaacg agtatgactt
     5041 tgtaccagag tctttcgata gagataagac cattgctctg atcatgaatt cctctggtag
     5101 tactggtcta ccaaagggtg ttgctctgcc acatagagca ttatgtgtca gatttagcca
     5161 tgctagagat cccatatttg gtaatcaaat tattccagac actgccattc tgtccgtcgt
     5221 tcctttccac catggttttg ggatgttcac aaccttgggt tacctaatct gtggttttag
     5281 agttatgttg atgtatcgtt ttgaagaaga gttgttcttg agatcattgc aagactacaa
     5341 aatacagaca gccctgttgg tcccaacact gttctctttc ttggctaaga gtaccctaat
     5401 agacaagtac gatttgagta acctacacga aatcgcatct ggaggtgcac cactttccaa
     5461 agaggttggt gaagctgtcg ccaaaagatt tcacttacct ggcattagac aaggttatgg
     5521 cctgacagag actactagtg ctatactgat tacccctgaa ggagatgata aacccggtgc
     5581 agttggcaaa gtggtcccat tctttgaagc aaaagttgta gacttagata ctggcaaaac
     5641 tcttggagtg aatcaacgtg gtgagttatg tgtacgtggt ccaatgatta tgtccggcta
     5701 cgtcaacaat ccagaagcaa caaatgcctt aatagacaaa gatggatggc tacattccgg
     5761 tgatatagcc tattgggatg aggacgaaca cttcttcatc gtagataggc ttaagtcttt
     5821 gatcaaatac aagggttatc aagttgctcc tgctgaattg gagtccattc tactacagca
     5881 tcctaacatc tttgatgctg gtgttgcagg tttaccagac gacgatgctg gtgaacttcc
     5941 agcggctgtg gttgtgttgg aacatggtaa aaccatgact gagaaggaaa tcgttgacta
     6001 cgtagcttca caagtaacta ccgcaaagaa actaagaggt ggagtagtct ttgtagacga
     6061 agttccaaag ggtttgacag gcaaattgga tgccagaaag attagggaaa tcttgattaa
     6121 agctaagaaa ggtggcaaga ttgctgttta aggtacccaa ttcgccctat agtgagtcgt
     6181 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6241 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6301 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     6361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6781 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     6841 ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc taatttgtga
     6901 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     6961 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     7021 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     7081 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     7141 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     7201 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     7261 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctgtca aaactagaga
     7321 ataataaaga aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct ttgttgttgt
     7381 tgtgggtgtg tgctatttat attatatagt ttactcataa taccataaaa tattcggttt
     7441 gattaggtta ttttaataag ctaatttgtt tctaatcgtg taatttatgc tgtgtatatt
     7501 aagtagtgtg tgcactgccc aaaaatgttt gttgtttata gtcggttaaa gagaaaaaag
     7561 aaaaaaagat ccatacacac acgttaatta gttgttcaac gtaatacact catattttgt
     7621 tcttatttgc tttcggtcgc tgttctcacc aagatttatt gccaacgaaa caattttttt
     7681 ttatatattt tcagattttt ctttttttcc tttcctttcc ttttctaatt ttcactcctg
     7741 gttttctttc tttcttagaa acattatctc gatattaata ttaaaaaaat ataatcattc
     7801 aaaatggacg gtggtatgtt ttagtttagc ttcaattcta attgattgat taatcagttg
     7861 attggtttca atatgacaaa tgggtagggt gggaaaactt cattttcaat tcagatcaaa
     7921 cttttttgtt gtcgacataa tatttctcgt ttgggatgtt actgtcacat taataataca
     7981 cacacatcag cttataattt tgaaagtaat ttatcagata tgttgtgacg atcagtggaa
     8041 atggctaact tcaatgtatc tgttcttccc ctttttcaaa gttcacgttt tttgattgat
     8101 tgattgatct gtcggcagtg gtttcaaaac cattcggtga gtaatcctat caatcaatgt
     8161 tacgacaaaa ggctcaatat tcaaaattgc aatgttttat gttttcctac gtgtacttgt
     8221 gcaaggcaat tgattcaaca ttgcttttgg tgtttgacga gtttctagtt tggacttgtg
     8281 ttgttatctg ggctatacag atttcccggc tcactatgaa tttttttttt cgacgctcag
     8341 tgcacacaac tataaacaac acaaacacaa acacagcaag aaaaaaaaaa aacgaacatt
     8401 gaattgaaac caagccaact gaaaaattcc ttatttaaat gact
//
