LOCUS       sequence-353599-        8934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse Myc with T58A point mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Mollaoglu G, Guthrie MR, Bohm S, Bragelmann J, Can I, Ballieu PM, 
            Marx A, George J, Heinen C, Chalishazar MD, Cheng H, Ireland AS, 
            Denning KE, Mukhopadhyay A, Vahrenkamp JM, Berrett KC, Mosbruger TL,
            Wang J, Kohan JL, Salama ME, Witt BL, Peifer M, Thomas RK, Gertz J, 
            Johnson JE, Gazdar AF, Wechsler-Reya RJ, Sos ML, Oliver TG
  TITLE     MYC Drives Progression of Small Cell Lung Cancer to a Variant 
            Neuroendocrine Subtype with Vulnerability to Aurora Kinase 
            Inhibition.
  JOURNAL   Cancer Cell. 2017 Feb 13;31(2):270-285. doi: 
            10.1016/j.ccell.2016.12.005. Epub 2017 Jan 12.
  PUBMED    28089889
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.12.005"; journalName: "Cancer Cell"; date: 
            "2017-02-13- 13"; volume: "31"; issue: "2"; pages: "270-285"
COMMENT     Sequence Label: PIG-MycT58A
FEATURES             Location/Qualifiers
     source          1..8934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             521..1120
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(521..540)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1017..1037
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1181..1748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1198..1750
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1342..1359)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1569..1588
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1762..1764,1765..1767,1768..2481)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1807..1828)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2068..2087)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2415..2436
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2583..3097
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3259..3275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3283..3289,3290..3307,3308..3313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3328..3349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3466..3483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3637..4225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3717..3736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4396..5187,5188..5256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5019..5038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5257..5361)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5429..5447
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5485..5507)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5607..5626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5820..5842
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5970..5989
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6189..6705
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6769..7110
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7177..7593
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7523..7545
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7561..7577
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
      541 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
      601 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
      661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
      721 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
      781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
      841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
      901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
      961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     1021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     1081 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     1141 gcgcccgacc gaaaggagcg cacgacccca tgcatccaat tccgcccccc ccccctaacg
     1201 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     1261 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     1321 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     1381 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     1441 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     1501 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     1561 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     1621 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     1681 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     1741 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1801 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag
     1861 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1921 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1981 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2041 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2161 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2221 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     2281 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2341 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2401 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2461 atggacgagc tgtacaagta atgaattaat taagaattat caagcttatc gataccgtcg
     2521 acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     2581 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     2641 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     2701 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     2761 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     2821 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     2881 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     2941 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
     3001 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     3061 ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg
     3121 agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg
     3181 ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc gcagaaagag
     3241 ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3301 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3421 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3481 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4441 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4501 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4561 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4621 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4681 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4741 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4801 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4861 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4921 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4981 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5041 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5101 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5161 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5221 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5281 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5341 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5401 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5461 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5521 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5581 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5641 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     5701 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5761 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5821 ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc
     5881 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     5941 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     6001 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa
     6061 tttgttaaag acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag
     6121 ctgaagccta tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa
     6181 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     6241 tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag
     6301 agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     6361 agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca
     6421 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     6481 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6541 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
     6601 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
     6661 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
     6721 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
     6781 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
     6841 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     6901 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     6961 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     7021 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     7081 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     7141 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     7201 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     7261 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     7321 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     7381 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     7441 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     7501 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     7561 ctttatccag ccctcactcc ttctctaggc gccggaatta gatctatgcc cctcaacgtg
     7621 aacttcacca acaggaacta tgacctcgac tacgactccg tacagcccta tttcatctgc
     7681 gacgaggaag agaatttcta tcaccagcaa cagcagagcg agctgcagcc gcccgcgccc
     7741 agtgaggata tctggaagaa attcgagctg cttcccgccc cgcccctgtc cccgagccgc
     7801 cgctccgggc tctgctctcc atcctatgtt gcggtcgcta cgtccttctc cccaagggaa
     7861 gacgatgacg gcggcggtgg caacttctcc accgccgatc agctggagat gatgaccgag
     7921 ttacttggag gagacatggt gaaccagagc ttcatctgcg atcctgacga cgagaccttc
     7981 atcaagaaca tcatcatcca ggactgtatg tggagcggtt tctcagccgc tgccaagctg
     8041 gtctcggaga agctggcctc ctaccaggct gcgcgcaaag acagcaccag cctgagcccc
     8101 gcccgcgggc acagcgtctg ctccacctcc agcctgtacc tgcaggacct caccgccgcc
     8161 gcgtccgagt gcattgaccc ctcagtggtc tttccctacc cgctcaacga cagcagctcg
     8221 cccaaatcct gtacctcgtc cgattccacg gccttctctc cttcctcgga ctcgctgctg
     8281 tcctccgagt cctccccacg ggccagccct gagcccctag tgctgcatga ggagacaccg
     8341 cccaccacca gcagcgactc tgaagaagag caagaagatg aggaagaaat tgatgtggtg
     8401 tctgtggaga agaggcaaac ccctgccaag aggtcggagt cgggctcatc tccatcccga
     8461 ggccacagca aacctccgca cagcccactg gtcctcaaga ggtgccacgt ctccactcac
     8521 cagcacaact acgccgcacc cccctccaca aggaaggact atccagctgc caagagggcc
     8581 aagttggaca gtggcagggt cctgaagcag atcagcaaca accgcaagtg ctccagcccc
     8641 aggtcctcag acacggagga aaacgacaag aggcggacac acaacgtctt ggaacgtcag
     8701 aggaggaacg agctgaagcg cagctttttt gccctgcgtg accagatccc tgaattggaa
     8761 aacaacgaaa aggcccccaa ggtagtgatc ctcaaaaaag ccaccgccta catcctgtcc
     8821 attcaagcag acgagcacaa gctcacctct gaaaaggact tattgaggaa acgacgagaa
     8881 cagttgaaac acaaactcga acagcttcga aactctggtg catagaattc tacc
//
