LOCUS       sequence-353602-        7320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression Recording Islands (XRIs) for recording cellular 
            physiological histories along intracellular protein self-assembly. 
            Contains XRI subunit with V5 tag, under c-fos promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C,
            Park D, Park WM, Ramirez S, Boyden ES
  TITLE     Recording of cellular physiological histories along optically 
            readable self-assembling protein chains.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7.
  PUBMED    36593405
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7."
COMMENT     Sequence Label: pAAV-cFos-XRI-V5
FEATURES             Location/Qualifiers
     source          1..7320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             complement(904..915)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3128..3169
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    4301..4508
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4678..4807
                     /label=AAV2 ITR
     rep_origin      complement(5044..5632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5124..5143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5803..6594,6595..6663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6426..6445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6664..6768)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6794..7249)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6931..6952)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7143..7162
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg taggttaatt aacaagcttt cctttaggaa cagaggcttc gagcctttaa
      241 ggctgcgtac ttgcttctcc taataccaga gactcaaaaa aaaaaaaaaa gttccagatt
      301 gctggacaat gacccgggtc tcatcccttg accctgggaa ccgggtccac attgaatcag
      361 gtgcgaatgt tcgctcgcct tctctgcctt tcccgcctcc cctcccccgg ccgcggcccc
      421 ggttcccccc ctgcgctgca ccctcagagt tggctgcagc cggcgagctg ttcccgtcaa
      481 tccctccctc ctttacacag gatgtccata ttaggacatc tgcgtcagca ggtttccacg
      541 gccggtccct gttgttctgg gggggggacc atctccgaaa tcctacacgc ggaaggtcta
      601 ggagaccccc taagatccca aatgtgaaca ctcataggtg aaagatgtat gccaagacgg
      661 gggttgaaag cctggggcgt agagttgacg acagagcgcc cgcagagggc cttggggcgc
      721 gcttcccccc ccttccagtt ccgcccagtg acgtaggaag tccatccatt cacagcgctt
      781 ctataaaggc gccagctgag gcgcctacta ctccaaccgc gactgcagcg agcaactgag
      841 aagactggat agagccggcg gttccgcgaa cgagcagtga ccgcgctccc acccagctct
      901 gctctgcagc tcccaccagt gtctacccct ggaccccttg ccgggctttc cccaaacttc
      961 gaccttctcg ggtttcaacg ccgactacga ggcgtcatcc tcccgctgca gtagcgcctc
     1021 cccggccggg gacagccttt cctactacca ttccccagcc gactccttct ccagcggctc
     1081 tcctgtcaac acacaggtga gtttggcttt gtgtagccgc caggtccgcg ctgagggtcg
     1141 ccgtggagga gacactgggg tgtgactcgc aggggcgggg gggtcttcct ttttcgctct
     1201 ggagggagac tggcgcggtc agagcagcct tagcctggga acccaggact tgtctgagcg
     1261 cgtgcacact tgtcatagta agacttagtg accccttccc gcgcggcagg tttattctga
     1321 gtggcctgcc tgcattcttc tctcggccga cttgtttctg agatcagccg gggccaacaa
     1381 gtctcgagca aagagtcgct aactagagtt tgggaggcgg caaaccgcgg caatcccccc
     1441 tcccggggca gcctggagca gggaggaggg aggagggagg agggtgctgc gggcgggtgt
     1501 gtaaggcagt ttcattgata aaaagcgagt tcattctgga gactccggag cagcgcctgc
     1561 gtcagcgcag acgtcaggga tatttataac aaaccccctt tcgagcgagt gatgccgaag
     1621 ggataacggg aacgcagcag taggatggag gagaaaggct gcgctgcgga attcaaggga
     1681 ggatattggg agagctttta tctccgatga ggtgcataca ggaagacata agcagtctct
     1741 gaccggaatg cttctctctc cctgcttcat gcgacactag ggccacttgc tccacctgtg
     1801 tctggaacct cctcgctcac ctccgctttc ctctttttgt tttgtttcag gacttttgcg
     1861 cagatcgata actagtgcca ccatgattga ctatactgct gctggtttca ccctcctcca
     1921 aggtgcacat ctgtacgctc ctgaagacag aggtatctgc gatgtgctgg ttgccaacgg
     1981 taagatcatc gctgtggcct ccaacattcc tagcgacatc gttccaaact gcaccgttgt
     2041 cgatctgagc ggacagatcc tctgtccagg attcattgat cagcacgtgc acctcattgg
     2101 aggaggaggc gaagctggtc ctactactag aactcccgaa gtcgcactgt ctcgcctcac
     2161 agaggcaggc gttacctccg tcgtgggact cctgggtact gatagcatct ctaggcaccc
     2221 tgagtctctg ctggcaaaga ccagagccct caatgaggag ggtatctccg cttggatgct
     2281 gactggtgct tatcacgtcc catctcgcac cattactggc agcgtggaga aggacgttgc
     2341 aatcatcgac agagtgatcg gtgttaagtg cgctatctcc gaccacagga gcgctgctcc
     2401 cgacgtctat cacctggcca acatggcagc cgagagcaga gtcggtggtc tgctcggtgg
     2461 caagccagga gttaccgtgt tccacatggg tgactctaag aaggcactgc aacccatcta
     2521 tgacctgctg gagaactgtg acgtcccaat ctccaagctg ctgcctaccc acgtcaacag
     2581 gaacgtgcct ctcttctacc aagctctgga gttcgcaaga aagggtggca ccatcgacat
     2641 cacctctagc attgatgaac ccgtcgctcc agctgaggga atcgcaaggg ctgtccaagc
     2701 aggtatccca ctggcaaggg tgacactcag ctctgatggc aacggctccc agccattctt
     2761 cgatgatgaa ggaaatctga ctcacattgg cgtggctgga tttgaaactc tcctggaaac
     2821 agttcaggtt ctggttaagg actacgactt cagcatttct gacgctctca gaccactcac
     2881 ctctagcgtc gctggcttcc tgaacctgac tggtaaaggc gaaatcctcc ctggtaatga
     2941 cgctgatctg ctggtgatga cacccgaact gcgcattgag caggtctatg caagaggaaa
     3001 gctgatggtt aaggacggaa aggcttgtgt caagggcaca ttcgagactg ctggtggctc
     3061 tggaggcacc ggagggtctg gagggactgg aggctctgga ggtactggcg gttctggagg
     3121 taccggtggt aagcctatcc ctaaccctct gctcggtctc gattctaccg gatccggtaa
     3181 gattgaagag ggcaaactgg ttatctggat caatggcgat aagggctaca atggcctggc
     3241 agaggtcggt aagaagttcg agaaagatac aggcatcaaa gttactgtgg aacacccaga
     3301 taagctggag gagaagttcc ctcaggtcgc agcaactggc gacggtccag acatcatctt
     3361 ctgggcacac gataggttcg gtggctatgc ccaatctggc ctgctggctg agattactcc
     3421 tgataaggct ttccaggaca agctgtatcc attcacctgg gatgctgtga gatacaatgg
     3481 caagctgatc gcatacccta ttgctgtgga agctctgagc ctgatctaca acaaggacct
     3541 gctgcctaat cctcctaaga catgggaaga aatccctgca ctggacaagg aactgaaggc
     3601 caaaggcaag tccgcactga tgttcaacct gcaggagcct tactttacct ggccactgat
     3661 tgctgccgac ggtggttacg ctttcaagta cgagaatggt aagtacgaca tcaaagatgt
     3721 gggtgtggac aatgctggtg ccaaagctgg tctgactttc ctggtggatc tgatcaagaa
     3781 caagcacatg aatgcagaca ctgactattc tatcgcagag gctgctttca acaaaggcga
     3841 aaccgcaatg actatcaatg gtccttgggc atggtctaac atcgacacta gcaaagtcaa
     3901 ctacggtgtc accgttctgc caaccttcaa gggtcagcca agcaaacctt tcgttggcgt
     3961 gctgagcgca ggtatcaacg ctgcctctcc taacaaagag ctggctaagg agtttctgga
     4021 gaactacctg ctgacagacg aaggtctgga ggcagtgaac aaggacaagc cactgggtgc
     4081 cgtggctctg aagagctacg aagaagaact ggccaaggac cctcgcatcg ctgctacaat
     4141 ggagaacgca cagaagggtg agatcatgcc aaacatccca cagatgagcg cattctggta
     4201 tgccgtgagg accgctgtta tcaacgcagc ttctggcaga cagaccgtgg atgaagccct
     4261 gaaagacgca cagacctaag cggccgctca taaagctcga ctgtgccttc tagttgccag
     4321 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4381 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4441 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4501 gctggggatg cggtccggac tgtacaattg cattcatttt atgtttcagg ttcaggggga
     4561 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     4621 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     4681 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4741 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4801 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5221 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5461 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6781 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     6841 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6901 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6961 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     7021 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     7081 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     7141 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     7201 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     7261 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
//
