LOCUS       sequence-353603-        5377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for human IFITM1. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, 
            Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun
            E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez 
            Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, 
            Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer 
            KMJ, Kirchhoff F
  TITLE     IFITM proteins promote SARS-CoV-2 infection and are targets for 
            virus inhibition in vitro.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4584.
  PUBMED    34321474
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1)"; pages: "4584"
COMMENT     Sequence Label: pCG_IFITM1
FEATURES             Location/Qualifiers
     source          1..5377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          1076..1648
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1780..1799)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1845..1900
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1899..1918)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      2254..2389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2495..2512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2666..3254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2746..2765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3425..4216,4217..4285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4048..4067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4286..4390)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4458..4476
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4514..4536)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4636..4655
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4722..5177)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4859..4880)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5071..5090
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5303..5325
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5317..5334
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5318..5334
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5341..5360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5341..5359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagaatat gcacaaggag gaacatgagg tggctgtgct gggggcaccc cccagcacca
      721 tccttccaag gtccaccgtg atcaacatcc acagcgagac ctccgtgccc gaccatgtcg
      781 tctggtccct gttcaacacc ctcttcttga actggtgctg tctgggcttc atagcattcg
      841 cctactccgt gaagtctagg gacaggaaga tggttggcga cgtgaccggg gcccaggcct
      901 atgcctccac cgccaagtgc ctgaacatct gggccctgat tctgggcatc ctcatgacca
      961 ttggattcat cctgttactg gtattcggct ctgtgacagt ctaccatatt atgttacaga
     1021 taatacagga aaaacggggt tactagtaga cgcgtcggat cctgagaact tcagggtgag
     1081 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
     1141 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
     1201 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1261 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
     1321 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca
     1381 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
     1441 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
     1501 tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata
     1561 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
     1621 tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc
     1681 tcatcatttt ggcaaagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     1741 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     1801 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     1861 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     1921 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     1981 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     2041 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     2101 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2161 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2221 gagatccctc gaggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cttcactact
     2281 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     2341 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     2401 ggggcgggac tatggttgct gactaattgc tgcattaatg aatcggccaa cgcgcgggga
     2461 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2521 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2581 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2641 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2701 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2761 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2821 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2881 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2941 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3001 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3061 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3121 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3181 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3241 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3301 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3361 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3421 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3481 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3541 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3601 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3661 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3721 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3781 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3841 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3901 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3961 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4021 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4081 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4141 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4201 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4261 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4321 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4381 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4441 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4501 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4561 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4621 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4681 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     4741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     5041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     5101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     5161 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5221 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5281 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     5341 taatacgact cactataggg cgaattcgag ctcgccc
//
