LOCUS       sequence-353607-        5399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for human IFITM2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, 
            Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun
            E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez 
            Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, 
            Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer 
            KMJ, Kirchhoff F
  TITLE     IFITM proteins promote SARS-CoV-2 infection and are targets for 
            virus inhibition in vitro.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4584.
  PUBMED    34321474
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1)"; pages: "4584"
COMMENT     Sequence Label: pCG_IFITM2
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          1098..1670
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1802..1821)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1867..1922
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1921..1940)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      2276..2411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2517..2534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2688..3276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2768..2787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3447..4238,4239..4307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4070..4089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4308..4412)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4480..4498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4536..4558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4658..4677
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4744..5199)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4881..4902)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5093..5112
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5325..5347
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5339..5356
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5340..5356
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5363..5382
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5363..5381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagaacca tgaaccacat tgtgcaaacc ttctctcctg tcaacagcgg ccagcctccc
      721 aactacgaga tgctcaagga ggagcaggaa gtggctatgc tgggggtgcc ccacaaccct
      781 gctcccccga tgtccaccgt gatccacatc cgcagcgaga cctccgtgcc tgaccatgtc
      841 gtctggtccc tgttcaacac cctcttcatg aacacctgct gcctgggctt catagcattc
      901 gcctactccg tgaagtctag ggacaggaag atggttggcg acgtgaccgg ggcccaggcc
      961 tatgcctcca ccgccaagtg cctgaacatc tgggccctga ttttgggcat cttcatgacc
     1021 attctgctca tcatcatccc agtgttggtc gtccaggccc agcgatagta gacgcgtcgg
     1081 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
     1141 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
     1201 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
     1261 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
     1321 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
     1381 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     1441 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     1501 gctggcgtgg aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt
     1561 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     1621 aaccgggccc ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcactcct caggtgcagg
     1741 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     1801 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     1861 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     1921 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     1981 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     2041 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     2101 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     2161 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     2221 tagctgtccc tcttctctta tggagatccc tcgaggagct ttttgcaaaa gcctaggcct
     2281 ccaaaaaagc ctcttcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     2341 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     2401 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gctgcattaa
     2461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2581 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2761 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2821 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2881 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2941 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3001 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3061 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3121 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3181 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3241 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3301 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3361 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3421 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3481 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3541 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3601 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3661 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3721 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3781 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3841 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3901 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3961 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4021 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4081 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4141 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4201 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4261 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4321 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4381 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4441 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4501 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4561 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4621 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4681 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     4741 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4801 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4861 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4921 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4981 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     5041 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     5101 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     5161 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     5221 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5281 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5341 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattcg agctcgccc
//
