LOCUS       sequence-353617-        6703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for human IFITM1. Co-expresses GFP controlled by 
            an IRES element..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, 
            Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun
            E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez 
            Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, 
            Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer 
            KMJ, Kirchhoff F
  TITLE     IFITM proteins promote SARS-CoV-2 infection and are targets for 
            virus inhibition in vitro.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4584.
  PUBMED    34321474
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1)"; pages: "4584"
COMMENT     Sequence Label: pCG_IFITM1-IRES_eGFP
FEATURES             Location/Qualifiers
     source          1..6703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     misc_feature    1093..1658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1109..1660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1253..1270)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1480..1499
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(1696..1724)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2319..2339
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          2402..2974
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3106..3125)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3171..3226
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3225..3244)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3580..3715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3821..3838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3992..4580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4072..4091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4751..5542,5543..5611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5374..5393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5612..5716)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5784..5802
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5840..5862)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5962..5981
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6048..6503)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6185..6206)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6397..6416
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6629..6651
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6643..6660
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6644..6660
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6667..6686
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6667..6685
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagaatat gcacaaggag gaacatgagg tggctgtgct gggggcaccc cccagcacca
      721 tccttccaag gtccaccgtg atcaacatcc acagcgagac ctccgtgccc gaccatgtcg
      781 tctggtccct gttcaacacc ctcttcttga actggtgctg tctgggcttc atagcattcg
      841 cctactccgt gaagtctagg gacaggaaga tggttggcga cgtgaccggg gcccaggcct
      901 atgcctccac cgccaagtgc ctgaacatct gggccctgat tctgggcatc ctcatgacca
      961 ttggattcat cctgttactg gtattcggct ctgtgacagt ctaccatatt atgttacaga
     1021 taatacagga aaaacggggt tactagtaga cgcgtcggat cccggagtac tacaaagact
     1081 gctgacgcga attccgcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     1141 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     1201 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     1261 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1321 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1381 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1441 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1501 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1561 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     1621 acggggacgt ggttttcctt tgaaaaacac gatgataata ccatggctag caaaggagaa
     1681 gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt taacggccac
     1741 aagttctctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact taccctgaag
     1801 ttcatctgca ctactggcaa actgcctgtt ccatggccaa cactagtcac tactctgtgc
     1861 tatggtgttc aatgcttttc aagatacccg gatcatatga aacggcatga ctttttcaag
     1921 agtgtcatgc ccgaaggtta tgtacaggaa aggaccatct tcttcaaaga tgacggcaac
     1981 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     2041 aaaggtattg acttcaagga agatggcaac attctgggac acaaattgga atacaactat
     2101 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agtgaacttc
     2161 aagacccgcc acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     2221 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     2281 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     2341 gctgctggga ttacacatgg catggatgaa ctgtacaact aaggatcctg agaacttcag
     2401 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
     2461 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
     2521 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
     2581 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
     2641 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     2701 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     2761 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     2821 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     2881 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     2941 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     3001 gctgtctcat cattttggca aagaattcac tcctcaggtg caggctgcct atcagaaggt
     3061 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     3121 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     3181 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     3241 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     3301 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     3361 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     3421 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     3481 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     3541 cttatggaga tccctcgagg agctttttgc aaaagcctag gcctccaaaa aagcctcttc
     3601 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3661 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3721 gagttagggg cgggactatg gttgctgact aattgctgca ttaatgaatc ggccaacgcg
     3781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5761 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     5821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     5881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     5941 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6001 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     6061 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6121 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6181 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6241 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6301 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6361 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6421 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6481 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     6541 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     6601 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     6661 gaattgtaat acgactcact atagggcgaa ttcgagctcg ccc
//
