LOCUS       sequence-353620-        6725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for human IFITM2. Co-expresses GFP controlled by 
            an IRES element..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, 
            Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun
            E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez 
            Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, 
            Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer 
            KMJ, Kirchhoff F
  TITLE     IFITM proteins promote SARS-CoV-2 infection and are targets for 
            virus inhibition in vitro.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4584.
  PUBMED    34321474
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1)"; pages: "4584"
COMMENT     Sequence Label: pCG_IFITM2-IRES_eGFP
FEATURES             Location/Qualifiers
     source          1..6725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     misc_feature    1115..1680
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1131..1682
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1275..1292)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1502..1521
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(1718..1746)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2341..2361
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          2424..2996
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3128..3147)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3193..3248
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3247..3266)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3602..3737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3843..3860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4014..4602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4094..4113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4773..5564,5565..5633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5396..5415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5634..5738)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5806..5824
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5862..5884)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5984..6003
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6070..6525)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6207..6228)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6419..6438
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6651..6673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6665..6682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6666..6682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6689..6708
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6689..6707
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagaacca tgaaccacat tgtgcaaacc ttctctcctg tcaacagcgg ccagcctccc
      721 aactacgaga tgctcaagga ggagcaggaa gtggctatgc tgggggtgcc ccacaaccct
      781 gctcccccga tgtccaccgt gatccacatc cgcagcgaga cctccgtgcc tgaccatgtc
      841 gtctggtccc tgttcaacac cctcttcatg aacacctgct gcctgggctt catagcattc
      901 gcctactccg tgaagtctag ggacaggaag atggttggcg acgtgaccgg ggcccaggcc
      961 tatgcctcca ccgccaagtg cctgaacatc tgggccctga ttttgggcat cttcatgacc
     1021 attctgctca tcatcatccc agtgttggtc gtccaggccc agcgatagta gacgcgtcgg
     1081 atcccggagt actacaaaga ctgctgacgc gaattccgcc ccccccccta acgttactgg
     1141 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     1201 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     1261 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     1321 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1381 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     1441 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     1501 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     1561 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     1621 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     1681 taccatggct agcaaaggag aagaactctt cactggagtt gtcccaattc ttgttgaatt
     1741 agatggtgat gttaacggcc acaagttctc tgtcagtgga gagggtgaag gtgatgcaac
     1801 atacggaaaa cttaccctga agttcatctg cactactggc aaactgcctg ttccatggcc
     1861 aacactagtc actactctgt gctatggtgt tcaatgcttt tcaagatacc cggatcatat
     1921 gaaacggcat gactttttca agagtgtcat gcccgaaggt tatgtacagg aaaggaccat
     1981 cttcttcaaa gatgacggca actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     2041 ccttgttaat agaatcgagt taaaaggtat tgacttcaag gaagatggca acattctggg
     2101 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     2161 gaatggaatc aaagtgaact tcaagacccg ccacaacatt gaagatggaa gcgttcaact
     2221 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     2281 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     2341 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactgtacaa
     2401 ctaaggatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg
     2461 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
     2521 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
     2581 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
     2641 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
     2701 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
     2761 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
     2821 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc
     2881 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
     2941 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc
     3001 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg
     3061 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     3121 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3181 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3241 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3301 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3361 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3421 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3481 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3541 cagtcatagc tgtccctctt ctcttatgga gatccctcga ggagcttttt gcaaaagcct
     3601 aggcctccaa aaaagcctct tcactacttc tggaatagct cagaggccga ggcggcctcg
     3661 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     3721 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgctg
     3781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5521 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5581 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5761 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     5821 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     5881 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     5941 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     6001 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6061 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6121 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6181 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6241 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6301 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6361 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6421 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     6481 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     6541 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6601 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6661 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattcgagct
     6721 cgccc
//
