LOCUS       sequence-353621-        9610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2830
FEATURES             Location/Qualifiers
     source          1..9610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2548..3136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2601..2621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3019..3030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3208..3441
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3519..3640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3556..3575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3610..3629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3680..3815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3835..3854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3836..3854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3864..3881)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3864..3880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3873..3895)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4022..4477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4109..4128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4319..4340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4503..4607
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4608..4676,4677..5468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4826..4845)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5639..6227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6128..6147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6381..6398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6515..6536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6551..6556,6557..6574,6575..6581)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6589..6605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6594..6616
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6613..6629
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6613..6629
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6648..6668
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6650..6668
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6696..6922
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6923..7103
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7150..7275
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7768..8001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8186..8230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8461..8671
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8483..8679
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8530..8665
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8711..9313
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8711..8730)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9207..9227
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9356..9473
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 agatatacaa acaaatgcgg aactgaaaag cactcaggag cagtctgtgc ccgcagaaag
     1261 tgcagcggtt ttgaatgact acagtttaac caaatctcat gaaatggaaa atgtggacag
     1321 tggagaaggc ccagccaatg aagatgaaga cataggagat gattcaatga aagtgaaaga
     1381 tgaatacagt gaaagagatg agaatgtttt aaagtcagaa cccatgggaa atgcagaaga
     1441 gcctgaaatc ccttacagct attcaagaga atataatgaa tatgaaaaca ttaagttgga
     1501 gagacatgtt gtctcattcg atagtagcag gccaaccagt ggaaagatga actgcgatgt
     1561 gtgtggatta tcctgcatca gcttcaatgt cttaatggtt cataagcgaa gccatactgc
     1621 aagtgcggag gcaagacaca tcaaagcaga gatgggaagt gaaagagctc tcgtactgga
     1681 cagattagca agcaatgtgg caaaacgaaa aagctcaatg cctcagaaat tcattggtga
     1741 gaagcgccac tgctttgatg tcaactataa ttcaagttac atgtatgaga aagagagtga
     1801 gctcatacag acccgcatga tggaccaagc catcaataac gccatcagct atcttggcgc
     1861 cgaagccctg cgccccttgg tccagacacc gcctgctccc acctcggaga tggttccagt
     1921 tatcagcagc atgtatccca tagccctcac ccgggctgag atgtcaaacg gtgcccctca
     1981 agagctggaa aagaaaagca tccaccttcc agagaagagc gtgccttctg agagaggcct
     2041 ctctcccaac aatagtggcc acgactccac ggacactgac agcaaccatg aagaacgcca
     2101 gaatcacatc tatcagcaaa atcacatggt cctgtctcgg gcccgcaatg ggatgccact
     2161 tctgaaggag gttccccgct cttacgaact cctcaagccc ccgcccatct gcccaagaga
     2221 ctccgtcaaa gtgatcaaca aggaagggga ggtgatggat gtgtatcggt gtgaccactg
     2281 ccgcgtcctc ttcctggact atgtgatgtt cacgattcac atgggctgcc acggcttccg
     2341 tgaccctttc gagtgtaaca tgtgtggata tcgaagccat gatcggtatg agttctcgtc
     2401 tcacatagcc agaggagaac acagagccct gctgaagtag taatgaacta gtccgtaact
     2461 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaat atacgcctac
     2521 cggtaggaac caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2941 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3121 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3181 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3241 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3421 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3481 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3781 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3841 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3901 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3961 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4021 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4081 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4141 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4201 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4261 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4321 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4381 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4441 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4501 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4561 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4621 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4681 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4741 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4801 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4861 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4921 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4981 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5041 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5101 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5161 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5221 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5281 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5341 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5401 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5461 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6481 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6541 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6601 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6661 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6721 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6781 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     6841 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     6901 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     6961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7081 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7141 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7201 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7261 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7321 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7381 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7441 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7501 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7561 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7621 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7681 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7741 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7801 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7861 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7921 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     7981 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8041 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8101 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8161 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8221 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8281 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8341 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8401 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8461 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     8521 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8581 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8641 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     8701 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8761 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     8821 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     8881 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     8941 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9001 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9061 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9121 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9181 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9241 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9301 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9361 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9421 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9481 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
     9541 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     9601 agtgggaatt
//
