LOCUS       sequence-353622-        9862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0957
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2800..3388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2853..2873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3271..3282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3460..3693
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3771..3892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3808..3827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3862..3881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3932..4067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4087..4106)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4088..4106)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4116..4133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4116..4132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4125..4147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4274..4729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4361..4380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4571..4592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4755..4859
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4860..4928,4929..5720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5078..5097)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5891..6479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6380..6399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6633..6650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6767..6788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6803..6808,6809..6826,6827..6833)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6841..6857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6846..6868
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6865..6881
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6865..6881
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6900..6920
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6902..6920
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6948..7174
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7175..7355
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7402..7527
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8020..8253
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8438..8482
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8713..8923
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8735..8931
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8782..8917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8963..9565
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8963..8982)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9459..9479
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9608..9725
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gtccccaaga gggtggaccc tgcaggtggc cccagaggaa ggccaggtcc tctgcaatgt
     1261 gaagactgcc acgaggggcc tctctgaggg ggctgtgtct ggaggctggg gggcctggga
     1321 aaactccacg gaggttccga gggaggcagg ggacggccag cggcagcaag ccacactggg
     1381 ggcggcggac gaacagggag gccccggcag ggagctgggc cccgcagacg gtgggcggga
     1441 cggggctggg cccaggagcg agcctgcaga ccgggcgttg cgcccttcgc ctctcccaga
     1501 ggagccgggc tgccggtgcg gggagtgcgg caaggcgttc agccagggct cttacttgct
     1561 gcagcatcgg cgcgtgcaca caggcgagaa gccgtacacg tgccccgagt gcggcaaggc
     1621 cttcgcctgg agctccaacc tcagccagca ccagcgcatc cacagcggcg agaagcccta
     1681 cgcttgcagg gagtgcggca aggccttccg cgcgcactcg cagctcatcc accaccagga
     1741 gacacacagc ggcctgaagc ccttccgctg cccggactgc ggcaagtcct tcggccgaag
     1801 caccacgctg gtgcagcacc gacgcacgca cacgggcgag aagccctacg agtgcccgga
     1861 gtgcggcaag gccttcagct ggaactccaa tttcctggag caccggcgcg tgcacacggg
     1921 cgcgcggccg cacgcctgcc gggactgtgg caaggccttc agccagagct ccaacctggc
     1981 cgagcacctg aagatccacg cgggcgcacg gccacacgcc tgtcccgact gcggcaaggc
     2041 cttcgtgcgt gtggcggggc tgcggcagca ccggcgcacg cacagcagcg agaagccctt
     2101 cccctgcgcc gagtgcggaa aggctttccg cgagagctcg cagctcctgc agcaccagcg
     2161 cacgcacact ggtgagcggc ccttcgagtg cgccgagtgc ggccaggctt tcgtcatggg
     2221 ctcctacctg gcggagcacc ggcgcgtgca cacgggcgag aagcctcatg cgtgcgccca
     2281 gtgcggcaag gccttcagcc agcgctccaa cctactgagc caccggcgca cgcactcggg
     2341 cgccaagccc ttcgcctgcg ccgactgcgg caaggccttc cgcggcagtt ccggcctggc
     2401 gcaccaccgg ctttcgcaca cgggagagcg acccttcgcc tgcgcagaat gcggcaaggc
     2461 cttccgcggc agctccgagc tgcgccagca ccagcgcctg cactctggcg agaggccgtt
     2521 cgtctgcgcc cactgcagca aggccttcgt gcgcaagtcg gagctcttaa gccaccggcg
     2581 cacgcacacg ggcgagaggc cctacgcttg cggcgagtgc gggaagcctt tcagccaccg
     2641 ttgcaacctc aacgagcacc agaagcggca cgggggccgc gctgcgccct agtaatgaac
     2701 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2761 gacatttggc gccgccatgt agtaacgcgt taagtcgaca atcaacctct ggattacaaa
     2821 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2881 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2941 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3001 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3061 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3121 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3181 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3241 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3301 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3361 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3421 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3481 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3661 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     3721 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3901 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3961 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4021 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4081 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4141 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4201 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4261 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4561 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4621 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4681 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4741 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4801 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4861 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4921 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4981 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5041 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5101 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5161 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5221 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5281 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5341 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5401 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5461 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5521 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5581 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5641 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5701 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5761 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5821 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5881 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5941 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6001 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6061 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6121 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6181 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6241 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6301 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6361 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6421 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6481 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6541 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6601 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6661 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6721 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6781 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6841 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6901 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6961 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7021 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7081 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7141 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7201 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7261 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7321 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7381 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7441 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7501 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7561 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7621 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7681 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7741 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7801 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7861 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7921 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7981 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8041 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8101 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8161 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8221 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8281 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8341 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8401 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8461 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8521 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8581 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8641 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     8701 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     8761 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8821 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8881 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     8941 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9001 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9061 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9121 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9181 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9241 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9301 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9361 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9421 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9481 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9541 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
     9601 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9661 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9721 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
     9781 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9841 ggtcttgaaa ggagtgggaa tt
//
