LOCUS       sequence-353625-       10458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNG^SEC^2xHA vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Duong T, Rasmussen NR, Ballato E, Mote FS, Reiner DJ
  TITLE     The Rheb-TORC1 signaling axis functions as a developmental 
            checkpoint.
  JOURNAL   Development. 2020 Mar 2;147(5). pii: dev.181727. doi: 
            10.1242/dev.181727.
  PUBMED    32041790
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-03-2"; volume: "147(5). pii: dev.181727.
            doi"; pages: " 10.1242/dev.181727"
COMMENT     Sequence Label: pTD31
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     144..162
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(200..222)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     322..341
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     535..557
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     549..566
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     550..566
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             832..1137
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      1515..1524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2070..2090
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    2108..2141
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             2151..2180
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3997..4320)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6238..6262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6265..7290
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6265..7290
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7291..7315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7668..7701
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7720..7746
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7747..7773
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8408..8424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8408..8424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8421..8443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8432..8448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8456..8462,8463..8480,8481..8486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8501..8522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8639..8656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8810..9398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8890..8909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9569..10360,10361..10429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10192..10211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10430..10458,1..76))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
       61 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
      121 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
      181 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      241 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      301 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
      361 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
      421 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      481 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      541 cacgacgttg taaaacgacg gccagtcgcc ggcactagtt acccgaagta tgtcaaaaag
      601 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
      661 aaggcatata tgatgtcaat atctccggtt cggtaagcac aaccatgcag aatgaagccc
      721 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
      781 ggtttattga aatgaacggc tcttttgccg acgagaacag gggctggtga aatgcagttt
      841 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
      901 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
      961 gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     1021 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     1081 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     1141 tcaggctccc ttatacacag ccagtctgca ggtcgaccta gggagcatcg ggagcctcag
     1201 gagcatcgat ggtctccaag ggagaggagg acaacatggc ctccctccca gccacccacg
     1261 agctccacat cttcggatcc atcaacggag tcgacttcga catggtcgga caaggaaccg
     1321 gaaacccaaa cgacggatac gaggagctca acctcaagtc caccaaggta agtttaaaca
     1381 tatatatact aactaaccct gattatttaa attttcaggg agacctccaa ttctccccat
     1441 ggatcctcgt cccacacatc ggatacggat tccaccaata cctcccatac ccagacggaa
     1501 tgtccccatt ccaagccgcc atggtcgacg gatccggata ccaagtccac cgtaccatgc
     1561 aattcgagga cggagcctcc ctcaccgtca actaccgtta cacctacgag ggatcccaca
     1621 tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagggag
     1681 aggcccaagt caagggaacc ggattcccag ccgacggacc agtcatgacc aactccctca
     1741 ccgccgccga ctggtgccgt tccaagaaga cctacccaaa cgacaaggta agtttaaaca
     1801 tgattttact aactaactaa tctgatttaa attttcagac catcatctcc accttcaagt
     1861 ggtcctacac caccggaaac ggaaagcgtt accgttccac cgcccgtacc acctacacct
     1921 tcgccaagcc aatggccgcc aactacctca agaaccaacc aatgtacgtc ttccgtaaga
     1981 ccgagctcaa gcactccaag accgagctca acttcaagga gtggcaaaag gccttcaccg
     2041 acgtcatggg aatggacgag ctctacaagg agaatctgta ctttcaatcc ggaaaggtaa
     2101 gtttaaaata acttcgtata atgtatacta tacgaagtta ttttcaggga gagcaaaagc
     2161 ttatctccga ggaggacctc taaggatgat cgacgccaac gtcgttgaat tttcaaattt
     2221 taaatactga atatttgttt tttttcctat tatttattta ttctctttgt gttttttttc
     2281 ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc tttccgtctc
     2341 ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt atcttctcgc
     2401 tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca aaaggatgat
     2461 ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata tggaggaagg
     2521 tttaaaagaa aacataaaaa tatatagagg aggaaggaaa ctagctgccc tcgattgtct
     2581 atcttccatt ctcaccaagt ttcaacaact ttattcagtc attcttctct ggttcatttc
     2641 ccaaccaatc ttcccgaata aagcccatct gtcctttcgg ccacattcct cacctacatc
     2701 cgtactcctt atctcccgtt cttatccagt cttttatcgt ctctgcgtct cgcacactgt
     2761 ctcgtcggta atgtaaaaaa aaaacaaaaa aaacttgaaa acttgcggaa agatttccta
     2821 ataaaaggag acgaatccgg taggaaatcg acaagtctct gcttctcgaa gactaggtga
     2881 gttacatttc tttggccgaa ttattttgaa atcctaaatt tgataatttc agagatgtct
     2941 gtaaaacttg cgtgttatgt gacggcttcg gtcactgtcg ccactcttat ggtatgcttc
     3001 atgaccatgt caaccatcta ctcggaggtt gatggattca gagagaagct tgataccgag
     3061 atgaatgtgt tcagagtgag ttaaattata ttttgaattt taaataattt ttaattttct
     3121 agcaatctac caatggattg tggaaggaca tagttgtcat cggaagatct agcaagcgtg
     3181 tccgttgtca atatgaagag accaacgcta ccccaactcc acatgctgat ggatccccat
     3241 ctgctccacc aggtcaacca ccagcagttc caccagtctt caaccagcca aagactccaa
     3301 atggagccaa tggaaatgga ccaacctgca actgcaatgc tgataacaag tgcccagctg
     3361 gaccatccgg accaaaggga gttccaggag ttccaggact cgacggagtt ccaggacttg
     3421 acggtgttcc aggagttgga gctgatgata tcgctccaca acgcgagtct gtcggatgct
     3481 tcacttgccc acaaggacca gttggaccac caggagctct tggaagacca ggaccacgtg
     3541 gacttccagg accaagagga caaaatggaa acccaggaag agatggacaa ccaggacatc
     3601 caggagagca aggatcatcc ggtcaaatcg gaaagatcgg agagccagga ccaccaggag
     3661 agaagggacg cgacgccgag catccaatcg gaagaccagg accaaaggga ccaagaggag
     3721 atcaaggacc aacaggacca gctggacaga acggtcttca cggaccacca ggagagccag
     3781 gaaccgttgg accagaagga ccatctggaa agcaaggacg tcaaggacca gacggaaccc
     3841 agggagagac tggaccagac ggaagaccag gaaaggatgc cgagtactgc cagtgcccag
     3901 acaagtctcc accatcagag gctgtcaacg ccaaccgtgg atacagaaat atctaaattg
     3961 ttggtgtttt ctaataaaat atttgagatt cagtgagact tttttcttgg cggcacaata
     4021 aagtttcctc tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca
     4081 tttttctcaa cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa
     4141 gtacatcaga cagaatcaca ttttgatagc attcacttca ctcagatgca aacttttcag
     4201 tgactataaa aagtcataaa ataataacat taaaaaagca aataaattag cgagagaatt
     4261 ttttgacaaa aagaaagaag agtgatagag aagaagggaa tgcttgaaag gattttgcat
     4321 ttatcttaat caccgtcttc gagaagacga accatggcgc cagtttcaga atccaagttt
     4381 ctaatatagt tcatcacaat gttgacgttg gtccaacctc cggcttgcat aatctctggg
     4441 atggaaactc cagcgcgagc catgtcacgg gcagctccga cacgggcgga atgtccagac
     4501 caagccaagt aacgttgtcc ggaatcatcc ttggctccgt agatgagacg gtgggtagct
     4561 tcgaagattc ctgaaaattt attttcagct tattcttagc taatatgttg tttaaactta
     4621 ccttcaagag ctcgagtcga gagttgtgat gttgcactcg gggcagcaac tccgttcttg
     4681 cgaacacgac agaacaggta gttgtttgga tcatcggcga ctccggaaac cgaaatccag
     4741 cgctcaacga gcttggtaac tccaagggag agcgcttttt cgactccggc agtcgagacc
     4801 agcgtttttg tacgtccgat gtggatgagc attcttcctc cgtctgtgcg actaatatcc
     4861 ttgacgcgaa tgcgggcaat ttcggcgata cggagaagtg tgttgtaagc aattccgagg
     4921 aaggcgaggt ttcggatgtc ctggcaacga tcggaatttt ccataagaga acggacctga
     4981 tcgaagtcgg ttctgaaaat ttcgtcaagt cttaggattg aatacattaa tatttaaact
     5041 tacctttcga aggcgagggc ttgcttggct ctctctccag catcaacatt ttccttgcgg
     5101 atacgtctca ttacgagaga tacggcatta gaatctgatg ggcgtggaag tccagatcgg
     5161 cgatggagca tattgagctg tccgagatgt tgttggatcg tcttcaccgc caatccacga
     5221 gcttggaggt agagcaagta gtcacgaacg tcttctggct cggctgggaa ccacttgcgg
     5281 ttgttaagct tgcaccaggc agcccaagaa cggcacactg agagaagcat cttccaggtg
     5341 tgctcggaga aggcttgtct gtcacggaac atatccatga ggttcttgcg aacttcatca
     5401 cttgtcgcgt caactggaag tgctggaaga ttttgatgaa cagtgagaag atttgacata
     5461 ttttcgaagt tttttagatg cactagaaca aagcgtgttg gcttcctctg agcccgcttt
     5521 ccttatatac ccgcattctg cagccttaca gaatgttcta gaaggtccta gatgcattcg
     5581 tttgaaaata ctcccggtgg gtgcaaagag acgcagacgg aaaatgtatc tgggtctctt
     5641 tattgtgtac actacttttc catgtaccga atgtgagtcg ccctcctttt gcaacaagca
     5701 gctcgaatgt tctagaaaaa ggtggaaaat agtataaata ccgttgaaaa taaataccga
     5761 acaacatttg ctctaattgt gaaattagaa atcttcaaac tataatcatg tcactttacc
     5821 actatttccg tccagctcaa cgttccgttt ttggtgatca tttttgcttt cgtcgtaaat
     5881 ctacacacgc gtctcttccg tgcgagagtc caagccagca gccaaattcg ttgactgagt
     5941 attcaacgtt tatacgttgt cggcaacgag aaataggaaa atgcatcggg aaatgttctt
     6001 ttttcgattt tttccaaggt tttgacaaat tttaccacga attttgctat gttttcaatt
     6061 aaaaaatatg ttattcaact gtttctatga ggaaaataag gctttgcatg taattttctt
     6121 attcagcata atttttaatt aatttgaatt ttctgtccta acgtttattt tgttttcttg
     6181 gttatgactg atctgaaatt aatttttgaa ttttaaggta atatgtcagg cggtgccgca
     6241 agtttgtaca aaaaagcagg ctccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     6301 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     6361 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     6421 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     6481 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     6541 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     6601 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     6661 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     6721 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     6781 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     6841 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     6901 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     6961 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     7021 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     7081 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     7141 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     7201 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     7261 cccagcactc gtccgagggc aaaggaatag acccagcttt cttgtacaaa gtgggtccaa
     7321 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     7381 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     7441 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     7501 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     7561 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     7621 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttaata acttcgtata
     7681 atgtatacta tacgaagtta ttttcaggga gccggctctt atccatacga tgtcccagat
     7741 tacgcttacc catatgacgt tccagactat gccggaggac tagttcgacc tgcagactgg
     7801 ctgtgtataa gggagcctga catttagatt ccccagaaca taaggttgat agcgttcttg
     7861 atatcgttct cacggtgaga aaggtcggca acctcctctc cgatgacgga aactggaacg
     7921 gaagccatgt cggtggtcat catacgccaa gactcgtctc cgatgtgaac aactgggtag
     7981 agctcacgtg gaaccttgtc ggaaagaaga cgggcggaag caagtgggat aaccatacgg
     8041 cgtcctgggg tatcgatgat gtcggattgg acgtcgacga aaagacggta gcgactctct
     8101 cttttataag tgtaaacctt gaattgcatt tcaccagccc ctgttctcgt cggcaaaaga
     8161 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
     8221 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttaccg aaccggagat
     8281 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
     8341 cgctgcttca tagcatacct ctttttgaca tacttcgggt tcctaggaaa tcgataccgg
     8401 taacatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9601 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9661 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9721 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9781 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9841 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9901 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9961 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10021 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10081 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10141 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10201 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10261 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10321 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10381 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10441 ttcaatatta ttgaagca
//
