LOCUS       sequence-353626-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNG^SEC^AID* vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Duong T, Rasmussen NR, Ballato E, Mote FS, Reiner DJ
  TITLE     The Rheb-TORC1 signaling axis functions as a developmental 
            checkpoint.
  JOURNAL   Development. 2020 Mar 2;147(5). pii: dev.181727. doi: 
            10.1242/dev.181727.
  PUBMED    32041790
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-03-2"; volume: "147(5). pii: dev.181727.
            doi"; pages: " 10.1242/dev.181727"
COMMENT     Sequence Label: pTD55
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..75)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     175..194
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     388..410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     402..419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     403..419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             685..990
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      1368..1377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1923..1943
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1961..1994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2004..2033
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3850..4173)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6091..6115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6118..7143
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6118..7143
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7144..7168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7521..7554)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7576..7707
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             complement(7752..8057)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8330..8346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8343..8365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8354..8370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8378..8384,8385..8402,8403..8408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8423..8444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8561..8578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8732..9320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8812..8831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9491..10282,10283..10351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10114..10133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10352..10456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(10524..10527,1..15)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
       61 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
      121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
      181 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
      241 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
      301 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
      361 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc
      421 gccggcacta gttacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
      481 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
      541 gttcggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
      601 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
      661 ccgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
      721 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
      781 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
      841 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
      901 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
      961 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     1021 gcaggtcgac ctagggagca tcgggagcct caggagcatc gatggtctcc aagggagagg
     1081 aggacaacat ggcctccctc ccagccaccc acgagctcca catcttcgga tccatcaacg
     1141 gagtcgactt cgacatggtc ggacaaggaa ccggaaaccc aaacgacgga tacgaggagc
     1201 tcaacctcaa gtccaccaag gtaagtttaa acatatatat actaactaac cctgattatt
     1261 taaattttca gggagacctc caattctccc catggatcct cgtcccacac atcggatacg
     1321 gattccacca atacctccca tacccagacg gaatgtcccc attccaagcc gccatggtcg
     1381 acggatccgg ataccaagtc caccgtacca tgcaattcga ggacggagcc tccctcaccg
     1441 tcaactaccg ttacacctac gagggatccc acatcaaggt aagtttaaac agttcggtac
     1501 taactaacca tacatattta aattttcagg gagaggccca agtcaaggga accggattcc
     1561 cagccgacgg accagtcatg accaactccc tcaccgccgc cgactggtgc cgttccaaga
     1621 agacctaccc aaacgacaag gtaagtttaa acatgatttt actaactaac taatctgatt
     1681 taaattttca gaccatcatc tccaccttca agtggtccta caccaccgga aacggaaagc
     1741 gttaccgttc caccgcccgt accacctaca ccttcgccaa gccaatggcc gccaactacc
     1801 tcaagaacca accaatgtac gtcttccgta agaccgagct caagcactcc aagaccgagc
     1861 tcaacttcaa ggagtggcaa aaggccttca ccgacgtcat gggaatggac gagctctaca
     1921 aggagaatct gtactttcaa tccggaaagg taagtttaaa ataacttcgt atagcataca
     1981 ttatacgaag ttattttcag ggagagcaaa agcttatctc cgaggaggac ctctaaggat
     2041 gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg ttttttttcc
     2101 tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc
     2161 aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct
     2221 gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac gtggggggaa
     2281 ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac acacaatatg
     2341 caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa aaatatatag
     2401 aggaggaagg aaactagctg ccctcgattg tctatcttcc attctcacca agtttcaaca
     2461 actttattca gtcattcttc tctggttcat ttcccaacca atcttcccga ataaagccca
     2521 tctgtccttt cggccacatt cctcacctac atccgtactc cttatctccc gttcttatcc
     2581 agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg gtaatgtaaa aaaaaaacaa
     2641 aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag gagacgaatc cggtaggaaa
     2701 tcgacaagtc tctgcttctc gaagactagg tgagttacat ttctttggcc gaattatttt
     2761 gaaatcctaa atttgataat ttcagagatg tctgtaaaac ttgcgtgtta tgtgacggct
     2821 tcggtcactg tcgccactct tatggtatgc ttcatgacca tgtcaaccat ctactcggag
     2881 gttgatggat tcagagagaa gcttgatacc gagatgaatg tgttcagagt gagttaaatt
     2941 atattttgaa ttttaaataa tttttaattt tctagcaatc taccaatgga ttgtggaagg
     3001 acatagttgt catcggaaga tctagcaagc gtgtccgttg tcaatatgaa gagaccaacg
     3061 ctaccccaac tccacatgct gatggatccc catctgctcc accaggtcaa ccaccagcag
     3121 ttccaccagt cttcaaccag ccaaagactc caaatggagc caatggaaat ggaccaacct
     3181 gcaactgcaa tgctgataac aagtgcccag ctggaccatc cggaccaaag ggagttccag
     3241 gagttccagg actcgacgga gttccaggac ttgacggtgt tccaggagtt ggagctgatg
     3301 atatcgctcc acaacgcgag tctgtcggat gcttcacttg cccacaagga ccagttggac
     3361 caccaggagc tcttggaaga ccaggaccac gtggacttcc aggaccaaga ggacaaaatg
     3421 gaaacccagg aagagatgga caaccaggac atccaggaga gcaaggatca tccggtcaaa
     3481 tcggaaagat cggagagcca ggaccaccag gagagaaggg acgcgacgcc gagcatccaa
     3541 tcggaagacc aggaccaaag ggaccaagag gagatcaagg accaacagga ccagctggac
     3601 agaacggtct tcacggacca ccaggagagc caggaaccgt tggaccagaa ggaccatctg
     3661 gaaagcaagg acgtcaagga ccagacggaa cccagggaga gactggacca gacggaagac
     3721 caggaaagga tgccgagtac tgccagtgcc cagacaagtc tccaccatca gaggctgtca
     3781 acgccaaccg tggatacaga aatatctaaa ttgttggtgt tttctaataa aatatttgag
     3841 attcagtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
     3901 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
     3961 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
     4021 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
     4081 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
     4141 gagaagaagg gaatgcttga aaggattttg catttatctt aatcaccgtc ttcgagaaga
     4201 cgaaccatgg cgccagtttc agaatccaag tttctaatat agttcatcac aatgttgacg
     4261 ttggtccaac ctccggcttg cataatctct gggatggaaa ctccagcgcg agccatgtca
     4321 cgggcagctc cgacacgggc ggaatgtcca gaccaagcca agtaacgttg tccggaatca
     4381 tccttggctc cgtagatgag acggtgggta gcttcgaaga ttcctgaaaa tttattttca
     4441 gcttattctt agctaatatg ttgtttaaac ttaccttcaa gagctcgagt cgagagttgt
     4501 gatgttgcac tcggggcagc aactccgttc ttgcgaacac gacagaacag gtagttgttt
     4561 ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa cgagcttggt aactccaagg
     4621 gagagcgctt tttcgactcc ggcagtcgag accagcgttt ttgtacgtcc gatgtggatg
     4681 agcattcttc ctccgtctgt gcgactaata tccttgacgc gaatgcgggc aatttcggcg
     4741 atacggagaa gtgtgttgta agcaattccg aggaaggcga ggtttcggat gtcctggcaa
     4801 cgatcggaat tttccataag agaacggacc tgatcgaagt cggttctgaa aatttcgtca
     4861 agtcttagga ttgaatacat taatatttaa acttaccttt cgaaggcgag ggcttgcttg
     4921 gctctctctc cagcatcaac attttccttg cggatacgtc tcattacgag agatacggca
     4981 ttagaatctg atgggcgtgg aagtccagat cggcgatgga gcatattgag ctgtccgaga
     5041 tgttgttgga tcgtcttcac cgccaatcca cgagcttgga ggtagagcaa gtagtcacga
     5101 acgtcttctg gctcggctgg gaaccacttg cggttgttaa gcttgcacca ggcagcccaa
     5161 gaacggcaca ctgagagaag catcttccag gtgtgctcgg agaaggcttg tctgtcacgg
     5221 aacatatcca tgaggttctt gcgaacttca tcacttgtcg cgtcaactgg aagtgctgga
     5281 agattttgat gaacagtgag aagatttgac atattttcga agttttttag atgcactaga
     5341 acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt ctgcagcctt
     5401 acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg tgggtgcaaa
     5461 gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt ttccatgtac
     5521 cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa aaaggtggaa
     5581 aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat tgtgaaatta
     5641 gaaatcttca aactataatc atgtcacttt accactattt ccgtccagct caacgttccg
     5701 tttttggtga tcatttttgc tttcgtcgta aatctacaca cgcgtctctt ccgtgcgaga
     5761 gtccaagcca gcagccaaat tcgttgactg agtattcaac gtttatacgt tgtcggcaac
     5821 gagaaatagg aaaatgcatc gggaaatgtt cttttttcga ttttttccaa ggttttgaca
     5881 aattttacca cgaattttgc tatgttttca attaaaaaat atgttattca actgtttcta
     5941 tgaggaaaat aaggctttgc atgtaatttt cttattcagc ataattttta attaatttga
     6001 attttctgtc ctaacgttta ttttgttttc ttggttatga ctgatctgaa attaattttt
     6061 gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt acaaaaaagc aggctccatg
     6121 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     6181 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     6241 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     6301 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     6361 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     6421 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     6481 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     6541 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     6601 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     6661 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     6721 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     6781 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     6841 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     6901 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     6961 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     7021 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     7081 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     7141 tagacccagc tttcttgtac aaagtgggtc caattactct tcaacatccc tacatgctct
     7201 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     7261 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     7321 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     7381 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     7441 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     7501 gtttatgacc gcaattttta ataacttcgt atagcataca ttatacgaag ttattttcag
     7561 ggagccggat ctatgcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     7621 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     7681 ggcccggagg cggcggcgtt cgtgaaacta gttcgacctg cagactggct gtgtataagg
     7741 gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
     7801 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
     7861 gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacggggg
     7921 actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg
     7981 tcaataatat cactctgtac atccacaaac agacgataac ggctctctct tttataggtg
     8041 taaaccttaa actgcatttc accagcccct gttctcgtcg gcaaaagagc cgttcatttc
     8101 aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg
     8161 cagacgacgg gcttcattct gcatggttgt gcttaccgaa ccggagatat tgacatcata
     8221 tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata
     8281 gcatacctct ttttgacata cttcgggttc ctaggaaatc gataacatgg tcatagctgt
     8341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8521 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8581 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8641 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10501 ttatcatgac attaacctat aaaaata
//
