LOCUS       sequence-353627-       10515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mKate2^SEC^AID* vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Reiner Lab
  TITLE     Reiner lab plasmids for self-excising CRISPR knock-in
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pTD56
FEATURES             Location/Qualifiers
     source          1..10515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..84
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(122..144)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     244..263
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     457..479
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     471..488
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     472..488
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             754..1059
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1980..2000
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(2018..2051)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2061..2090
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3907..4230)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6148..6172
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6175..7200
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6175..7200
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7201..7225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7578..7611)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7633..7764
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             complement(7809..8114)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8387..8403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8387..8403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8400..8422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8411..8427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8435..8441,8442..8459,8460..8465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8480..8501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8618..8635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8789..9377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8869..8888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9548..10339,10340..10408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10171..10190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10409..10513)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
       61 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
      121 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      241 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
      301 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
      361 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      421 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      481 cggccagtcg ccggcactag ttacccgaag tatgtcaaaa agaggtatgc tatgaagcag
      541 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
      601 atatctccgg ttcggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
      661 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
      721 gctcttttgc cgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
      781 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
      841 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa
      901 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
      961 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1021 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     1081 agccagtctg caggtcgacc tagggagcat cgggagcctc aggagcatcg atggtctccg
     1141 agctcattaa agaaaacatg catatgaagc tctacatgga gggaaccgtc aacaaccacc
     1201 acttcaagtg cacctccgag ggagagggaa agccatacga gggaacccaa accatgcgta
     1261 tcaaggccgt cgagggagga ccactcccat tcgccttcga catcctcgcc acctccttca
     1321 tgtacggatc caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     1381 ttcagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa tccttcccag
     1441 agggattcac ctgggagcgt gtcaccacct acgaggacgg aggagtcctc accgccaccc
     1501 aagacacctc cctccaagac ggatgcctca tctacaacgt caaggtaagt ttaaacagtt
     1561 cggtactaac taaccataca tatttaaatt ttcagatccg tggagtcaac ttcccatcca
     1621 acggaccagt catgcaaaag aagaccctcg gatgggaggc ctccaccgag accctctacc
     1681 cagccgacgg aggacttgag ggacgtgccg acatggccct caagctcgtc ggaggaggac
     1741 acctcatctg caacctcaag gtaagtttaa acatgatttt actaactaac taatctgatt
     1801 taaattttca gaccacctac cgttccaaga agccagccaa gaacctcaag atgccaggag
     1861 tctactacgt cgaccgtcgt cttgagcgta tcaaggaggc cgacaaggag acctacgtcg
     1921 agcaacacga ggtcgccgtc gcccgttact gcgacctccc atccaagctc ggacaccgtg
     1981 agaatctgta ctttcaatcc ggaaaggtaa gtttaaaata acttcgtata gcatacatta
     2041 tacgaagtta ttttcaggga gagcaaaagc ttatctccga ggaggacctc taaggatgat
     2101 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     2161 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     2221 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     2281 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     2341 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     2401 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     2461 aggaaggaaa ctagctgccc tcgattgtct atcttccatt ctcaccaagt ttcaacaact
     2521 ttattcagtc attcttctct ggttcatttc ccaaccaatc ttcccgaata aagcccatct
     2581 gtcctttcgg ccacattcct cacctacatc cgtactcctt atctcccgtt cttatccagt
     2641 cttttatcgt ctctgcgtct cgcacactgt ctcgtcggta atgtaaaaaa aaaacaaaaa
     2701 aaacttgaaa acttgcggaa agatttccta ataaaaggag acgaatccgg taggaaatcg
     2761 acaagtctct gcttctcgaa gactaggtga gttacatttc tttggccgaa ttattttgaa
     2821 atcctaaatt tgataatttc agagatgtct gtaaaacttg cgtgttatgt gacggcttcg
     2881 gtcactgtcg ccactcttat ggtatgcttc atgaccatgt caaccatcta ctcggaggtt
     2941 gatggattca gagagaagct tgataccgag atgaatgtgt tcagagtgag ttaaattata
     3001 ttttgaattt taaataattt ttaattttct agcaatctac caatggattg tggaaggaca
     3061 tagttgtcat cggaagatct agcaagcgtg tccgttgtca atatgaagag accaacgcta
     3121 ccccaactcc acatgctgat ggatccccat ctgctccacc aggtcaacca ccagcagttc
     3181 caccagtctt caaccagcca aagactccaa atggagccaa tggaaatgga ccaacctgca
     3241 actgcaatgc tgataacaag tgcccagctg gaccatccgg accaaaggga gttccaggag
     3301 ttccaggact cgacggagtt ccaggacttg acggtgttcc aggagttgga gctgatgata
     3361 tcgctccaca acgcgagtct gtcggatgct tcacttgccc acaaggacca gttggaccac
     3421 caggagctct tggaagacca ggaccacgtg gacttccagg accaagagga caaaatggaa
     3481 acccaggaag agatggacaa ccaggacatc caggagagca aggatcatcc ggtcaaatcg
     3541 gaaagatcgg agagccagga ccaccaggag agaagggacg cgacgccgag catccaatcg
     3601 gaagaccagg accaaaggga ccaagaggag atcaaggacc aacaggacca gctggacaga
     3661 acggtcttca cggaccacca ggagagccag gaaccgttgg accagaagga ccatctggaa
     3721 agcaaggacg tcaaggacca gacggaaccc agggagagac tggaccagac ggaagaccag
     3781 gaaaggatgc cgagtactgc cagtgcccag acaagtctcc accatcagag gctgtcaacg
     3841 ccaaccgtgg atacagaaat atctaaattg ttggtgtttt ctaataaaat atttgagatt
     3901 cagtgagact tttttcttgg cggcacaata aagtttcctc tttttgttgg gtcactcaac
     3961 gaacacaata caaatatttg agcgctcgca tttttctcaa cctttcgggt ttaaagcact
     4021 tcaaaatgga attgaagaga gattgtgaaa gtacatcaga cagaatcaca ttttgatagc
     4081 attcacttca ctcagatgca aacttttcag tgactataaa aagtcataaa ataataacat
     4141 taaaaaagca aataaattag cgagagaatt ttttgacaaa aagaaagaag agtgatagag
     4201 aagaagggaa tgcttgaaag gattttgcat ttatcttaat caccgtcttc gagaagacga
     4261 accatggcgc cagtttcaga atccaagttt ctaatatagt tcatcacaat gttgacgttg
     4321 gtccaacctc cggcttgcat aatctctggg atggaaactc cagcgcgagc catgtcacgg
     4381 gcagctccga cacgggcgga atgtccagac caagccaagt aacgttgtcc ggaatcatcc
     4441 ttggctccgt agatgagacg gtgggtagct tcgaagattc ctgaaaattt attttcagct
     4501 tattcttagc taatatgttg tttaaactta ccttcaagag ctcgagtcga gagttgtgat
     4561 gttgcactcg gggcagcaac tccgttcttg cgaacacgac agaacaggta gttgtttgga
     4621 tcatcggcga ctccggaaac cgaaatccag cgctcaacga gcttggtaac tccaagggag
     4681 agcgcttttt cgactccggc agtcgagacc agcgtttttg tacgtccgat gtggatgagc
     4741 attcttcctc cgtctgtgcg actaatatcc ttgacgcgaa tgcgggcaat ttcggcgata
     4801 cggagaagtg tgttgtaagc aattccgagg aaggcgaggt ttcggatgtc ctggcaacga
     4861 tcggaatttt ccataagaga acggacctga tcgaagtcgg ttctgaaaat ttcgtcaagt
     4921 cttaggattg aatacattaa tatttaaact tacctttcga aggcgagggc ttgcttggct
     4981 ctctctccag catcaacatt ttccttgcgg atacgtctca ttacgagaga tacggcatta
     5041 gaatctgatg ggcgtggaag tccagatcgg cgatggagca tattgagctg tccgagatgt
     5101 tgttggatcg tcttcaccgc caatccacga gcttggaggt agagcaagta gtcacgaacg
     5161 tcttctggct cggctgggaa ccacttgcgg ttgttaagct tgcaccaggc agcccaagaa
     5221 cggcacactg agagaagcat cttccaggtg tgctcggaga aggcttgtct gtcacggaac
     5281 atatccatga ggttcttgcg aacttcatca cttgtcgcgt caactggaag tgctggaaga
     5341 ttttgatgaa cagtgagaag atttgacata ttttcgaagt tttttagatg cactagaaca
     5401 aagcgtgttg gcttcctctg agcccgcttt ccttatatac ccgcattctg cagccttaca
     5461 gaatgttcta gaaggtccta gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag
     5521 acgcagacgg aaaatgtatc tgggtctctt tattgtgtac actacttttc catgtaccga
     5581 atgtgagtcg ccctcctttt gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat
     5641 agtataaata ccgttgaaaa taaataccga acaacatttg ctctaattgt gaaattagaa
     5701 atcttcaaac tataatcatg tcactttacc actatttccg tccagctcaa cgttccgttt
     5761 ttggtgatca tttttgcttt cgtcgtaaat ctacacacgc gtctcttccg tgcgagagtc
     5821 caagccagca gccaaattcg ttgactgagt attcaacgtt tatacgttgt cggcaacgag
     5881 aaataggaaa atgcatcggg aaatgttctt ttttcgattt tttccaaggt tttgacaaat
     5941 tttaccacga attttgctat gttttcaatt aaaaaatatg ttattcaact gtttctatga
     6001 ggaaaataag gctttgcatg taattttctt attcagcata atttttaatt aatttgaatt
     6061 ttctgtccta acgtttattt tgttttcttg gttatgactg atctgaaatt aatttttgaa
     6121 ttttaaggta atatgtcagg cggtgccgca agtttgtaca aaaaagcagg ctccatgaaa
     6181 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     6241 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     6301 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     6361 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     6421 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     6481 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     6541 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     6601 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     6661 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     6721 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     6781 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     6841 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     6901 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     6961 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     7021 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     7081 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     7141 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     7201 acccagcttt cttgtacaaa gtgggtccaa ttactcttca acatccctac atgctctttc
     7261 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     7321 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     7381 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     7441 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     7501 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     7561 tatgaccgca atttttaata acttcgtata gcatacatta tacgaagtta ttttcaggga
     7621 gccggatcta tgcctaaaga tccagccaaa cctccggcca aggcacaagt tgtgggatgg
     7681 ccaccggtga gatcataccg gaagaacgtg atggtttcct gccaaaaatc aagcggtggc
     7741 ccggaggcgg cggcgttcgt gaaactagtt cgacctgcag actggctgtg tataagggag
     7801 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
     7861 tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg
     7921 gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggggact
     7981 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
     8041 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
     8101 accttaaact gcatttcacc agcccctgtt ctcgtcggca aaagagccgt tcatttcaat
     8161 aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag
     8221 acgacgggct tcattctgca tggttgtgct taccgaaccg gagatattga catcatatat
     8281 gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca
     8341 tacctctttt tgacatactt cgggttccta ggaaatcgat aacatggtca tagctgtttc
     8401 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8461 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8581 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8641 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8701 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9241 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9301 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9541 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9601 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9661 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9721 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9781 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9841 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9901 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9961 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10021 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10081 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10141 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10201 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10261 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10321 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10381 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10441 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10501 taggggttcc gcgca
//
