LOCUS       sequence-353630-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mKate2^SEC^2xHA vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Reiner Lab
  TITLE     Reiner lab plasmids for self-excising CRISPR knock-in
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pTD72
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(524..1315,1316..1384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1147..1166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1385..1489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1557..1575
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1613..1635)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1735..1754
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1948..1970
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1962..1979
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1963..1979
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2245..2550
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             3471..3491
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    3509..3542
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             3552..3581
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(5398..5721)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7639..7663
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7666..8691
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7666..8691
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8692..8716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    9069..9102
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             9121..9147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9148..9174
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9809..9825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9809..9825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9822..9844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9833..9849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9857..9863,9864..9881,9882..9887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9902..9923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10040..10057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10211..10446,1..353))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10291..10310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
       61 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1501 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1561 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1621 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1681 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1741 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1801 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1861 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1921 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc
     1981 gccggcacta gttacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2041 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2101 gttcggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2161 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2221 ccgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2281 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2341 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2401 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2461 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2521 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2581 gcaggtcgac ctagggagca tcgggagcct caggagcatc gatggtctcc gagctcatta
     2641 aagaaaacat gcatatgaag ctctacatgg agggaaccgt caacaaccac cacttcaagt
     2701 gcacctccga gggagaggga aagccatacg agggaaccca aaccatgcgt atcaaggccg
     2761 tcgagggagg accactccca ttcgccttcg acatcctcgc cacctccttc atgtacggat
     2821 ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagacct
     2881 tcatcaacca cacccaagga atcccagact tcttcaagca atccttccca gagggattca
     2941 cctgggagcg tgtcaccacc tacgaggacg gaggagtcct caccgccacc caagacacct
     3001 ccctccaaga cggatgcctc atctacaacg tcaaggtaag tttaaacagt tcggtactaa
     3061 ctaaccatac atatttaaat tttcagatcc gtggagtcaa cttcccatcc aacggaccag
     3121 tcatgcaaaa gaagaccctc ggatgggagg cctccaccga gaccctctac ccagccgacg
     3181 gaggacttga gggacgtgcc gacatggccc tcaagctcgt cggaggagga cacctcatct
     3241 gcaacctcaa ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
     3301 agaccaccta ccgttccaag aagccagcca agaacctcaa gatgccagga gtctactacg
     3361 tcgaccgtcg tcttgagcgt atcaaggagg ccgacaagga gacctacgtc gagcaacacg
     3421 aggtcgccgt cgcccgttac tgcgacctcc catccaagct cggacaccgt gagaatctgt
     3481 actttcaatc cggaaaggta agtttaaaat aacttcgtat aatgtatact atacgaagtt
     3541 attttcaggg agagcaaaag cttatctccg aggaggacct ctaaggatga tcgacgccaa
     3601 cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt
     3661 attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat
     3721 ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg
     3781 caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg
     3841 ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca
     3901 attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa
     3961 actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac tttattcagt
     4021 cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc tgtcctttcg
     4081 gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag tcttttatcg
     4141 tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa aaaacttgaa
     4201 aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc gacaagtctc
     4261 tgcttctcga agactaggtg agttacattt ctttggccga attattttga aatcctaaat
     4321 ttgataattt cagagatgtc tgtaaaactt gcgtgttatg tgacggcttc ggtcactgtc
     4381 gccactctta tggtatgctt catgaccatg tcaaccatct actcggaggt tgatggattc
     4441 agagagaagc ttgataccga gatgaatgtg ttcagagtga gttaaattat attttgaatt
     4501 ttaaataatt tttaattttc tagcaatcta ccaatggatt gtggaaggac atagttgtca
     4561 tcggaagatc tagcaagcgt gtccgttgtc aatatgaaga gaccaacgct accccaactc
     4621 cacatgctga tggatcccca tctgctccac caggtcaacc accagcagtt ccaccagtct
     4681 tcaaccagcc aaagactcca aatggagcca atggaaatgg accaacctgc aactgcaatg
     4741 ctgataacaa gtgcccagct ggaccatccg gaccaaaggg agttccagga gttccaggac
     4801 tcgacggagt tccaggactt gacggtgttc caggagttgg agctgatgat atcgctccac
     4861 aacgcgagtc tgtcggatgc ttcacttgcc cacaaggacc agttggacca ccaggagctc
     4921 ttggaagacc aggaccacgt ggacttccag gaccaagagg acaaaatgga aacccaggaa
     4981 gagatggaca accaggacat ccaggagagc aaggatcatc cggtcaaatc ggaaagatcg
     5041 gagagccagg accaccagga gagaagggac gcgacgccga gcatccaatc ggaagaccag
     5101 gaccaaaggg accaagagga gatcaaggac caacaggacc agctggacag aacggtcttc
     5161 acggaccacc aggagagcca ggaaccgttg gaccagaagg accatctgga aagcaaggac
     5221 gtcaaggacc agacggaacc cagggagaga ctggaccaga cggaagacca ggaaaggatg
     5281 ccgagtactg ccagtgccca gacaagtctc caccatcaga ggctgtcaac gccaaccgtg
     5341 gatacagaaa tatctaaatt gttggtgttt tctaataaaa tatttgagat tcagtgagac
     5401 ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa cgaacacaat
     5461 acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac ttcaaaatgg
     5521 aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag cattcacttc
     5581 actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca ttaaaaaagc
     5641 aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga gaagaaggga
     5701 atgcttgaaa ggattttgca tttatcttaa tcaccgtctt cgagaagacg aaccatggcg
     5761 ccagtttcag aatccaagtt tctaatatag ttcatcacaa tgttgacgtt ggtccaacct
     5821 ccggcttgca taatctctgg gatggaaact ccagcgcgag ccatgtcacg ggcagctccg
     5881 acacgggcgg aatgtccaga ccaagccaag taacgttgtc cggaatcatc cttggctccg
     5941 tagatgagac ggtgggtagc ttcgaagatt cctgaaaatt tattttcagc ttattcttag
     6001 ctaatatgtt gtttaaactt accttcaaga gctcgagtcg agagttgtga tgttgcactc
     6061 ggggcagcaa ctccgttctt gcgaacacga cagaacaggt agttgtttgg atcatcggcg
     6121 actccggaaa ccgaaatcca gcgctcaacg agcttggtaa ctccaaggga gagcgctttt
     6181 tcgactccgg cagtcgagac cagcgttttt gtacgtccga tgtggatgag cattcttcct
     6241 ccgtctgtgc gactaatatc cttgacgcga atgcgggcaa tttcggcgat acggagaagt
     6301 gtgttgtaag caattccgag gaaggcgagg tttcggatgt cctggcaacg atcggaattt
     6361 tccataagag aacggacctg atcgaagtcg gttctgaaaa tttcgtcaag tcttaggatt
     6421 gaatacatta atatttaaac ttacctttcg aaggcgaggg cttgcttggc tctctctcca
     6481 gcatcaacat tttccttgcg gatacgtctc attacgagag atacggcatt agaatctgat
     6541 gggcgtggaa gtccagatcg gcgatggagc atattgagct gtccgagatg ttgttggatc
     6601 gtcttcaccg ccaatccacg agcttggagg tagagcaagt agtcacgaac gtcttctggc
     6661 tcggctggga accacttgcg gttgttaagc ttgcaccagg cagcccaaga acggcacact
     6721 gagagaagca tcttccaggt gtgctcggag aaggcttgtc tgtcacggaa catatccatg
     6781 aggttcttgc gaacttcatc acttgtcgcg tcaactggaa gtgctggaag attttgatga
     6841 acagtgagaa gatttgacat attttcgaag ttttttagat gcactagaac aaagcgtgtt
     6901 ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac agaatgttct
     6961 agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga gacgcagacg
     7021 gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg aatgtgagtc
     7081 gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa tagtataaat
     7141 accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga aatcttcaaa
     7201 ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt tttggtgatc
     7261 atttttgctt tcgtcgtaaa tctacacacg cgtctcttcc gtgcgagagt ccaagccagc
     7321 agccaaattc gttgactgag tattcaacgt ttatacgttg tcggcaacga gaaataggaa
     7381 aatgcatcgg gaaatgttct tttttcgatt ttttccaagg ttttgacaaa ttttaccacg
     7441 aattttgcta tgttttcaat taaaaaatat gttattcaac tgtttctatg aggaaaataa
     7501 ggctttgcat gtaattttct tattcagcat aatttttaat taatttgaat tttctgtcct
     7561 aacgtttatt ttgttttctt ggttatgact gatctgaaat taatttttga attttaaggt
     7621 aatatgtcag gcggtgccgc aagtttgtac aaaaaagcag gctccatgaa aaagcctgaa
     7681 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     7741 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     7801 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     7861 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     7921 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     7981 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     8041 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     8101 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     8161 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     8221 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     8281 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     8341 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     8401 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     8461 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     8521 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     8581 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     8641 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gacccagctt
     8701 tcttgtacaa agtgggtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     8761 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     8821 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     8881 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     8941 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     9001 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     9061 aatttttaat aacttcgtat aatgtatact atacgaagtt attttcaggg agccggctct
     9121 tatccatacg atgtcccaga ttacgcttac ccatatgacg ttccagacta tgccggagga
     9181 ctagttcgac ctgcagactg gctgtgtata agggagcctg acatttagat tccccagaac
     9241 ataaggttga tagcgttctt gatatcgttc tcacggtgag aaaggtcggc aacctcctct
     9301 ccgatgacgg aaactggaac ggaagccatg tcggtggtca tcatacgcca agactcgtct
     9361 ccgatgtgaa caactgggta gagctcacgt ggaaccttgt cggaaagaag acgggcggaa
     9421 gcaagtggga taaccatacg gcgtcctggg gtatcgatga tgtcggattg gacgtcgacg
     9481 aaaagacggt agcgactctc tcttttataa gtgtaaacct tgaattgcat ttcaccagcc
     9541 cctgttctcg tcggcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     9601 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     9661 tgtgcttacc gaaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
     9721 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
     9781 ttcctaggaa atcgataccg gtaacatggt catagctgtt tcctgtgtga aattgttatc
     9841 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9901 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9961 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10021 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10081 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10141 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10201 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10381 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10441 tcgttc
//
