LOCUS       sequence-353634-        2272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XI-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XI2up
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             384..1043
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(450..469)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1114..1148
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(join(1886..2272,1..202))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1966..1985)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
       61 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      241 gtggaacgaa aactcacgtt aagggatttt ggtcatgatt gatcgggcac gtaagaggtt
      301 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      361 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      421 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      481 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      541 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      601 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      661 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      721 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      781 atttccctaa agggtttatt gagaatatgt ttttcgtttc agccaatccc tgggtgagtt
      841 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      901 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      961 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     1021 atgagtggca gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat
     1081 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     1141 ttttttattg gtctggtctc aagggcgagc tcgctgagga cttaatttgt ggaagttcat
     1201 ggcaaacgct ccttggcttt tgcctagtct tgttctatgg cacatttttc tgttgagtta
     1261 caccccgcgt gctaaaaaag gggtgaacca tcagaaagct tattactgag ttttgcggag
     1321 catcgctcgg agcggcggaa ttgaatcgaa ccgccgtgct attaccgaac aaaaaaattc
     1381 gaaagcataa actcagtagt gaaaaacttg agaattttca gatgagtggc gactttccag
     1441 tccttgcggt tttgtcacct tagtcagcta gtaaggaggc cgtgtgggtt agagtggcta
     1501 caatcctcaa agggcacttc tagaacccac ggtgaatttt ttttggcatg ataaatcggt
     1561 agaatcggtg aagtaattac ccaaaaaagg atcgggattg tgtttctcgt aattccgtat
     1621 tattgccgat ggcatcgact acttcttttt tcagaaaccc caacaagggt ctattgtaat
     1681 tgtatataaa cctttttgta atggatatat acatgtggta ctatttctcc tcatcctgct
     1741 ccatcgaaaa tcctcattaa accctcagcg cattgtgaga ccgactacgg ttatccacag
     1801 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gt
//
