LOCUS       sequence-353635-        9757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0285
FEATURES             Location/Qualifiers
     source          1..9757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2695..3283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2748..2768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3166..3177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3355..3588
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3666..3787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3703..3722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3757..3776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3827..3962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3982..4001)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3983..4001)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4011..4028)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4011..4027)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4020..4042)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4169..4624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4256..4275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4466..4487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4650..4754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4755..4823,4824..5615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4973..4992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5786..6374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6275..6294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6528..6545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6662..6683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6698..6703,6704..6721,6722..6728)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6736..6752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6741..6763
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6760..6776
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6760..6776
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6795..6815
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6797..6815
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6843..7069
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7070..7250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7297..7422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7915..8148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8333..8377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8608..8818
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8630..8826
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8677..8812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8858..9460
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8858..8877)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9354..9374
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9503..9620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 gagaaaaaag attcagatta cgaggattat ggatgaacgt aacagacagg tgacatttac
     1261 aaagaggaaa tttgggttga tgaagaaggc ttatgagctg agcgtgctgt gtgactgtga
     1321 gattgcgctg atcatcttca acagcaccaa caagctgttc cagtatgcca gcaccgacat
     1381 ggacaaagtg cttctcaagt acacggagta caacgagccg catgagagcc ggacaaactc
     1441 agacatcgtg gaggcattga acaagaaaga aaacaaaggc tgtgaaagcc ccgatcccga
     1501 ctcctcttat gcactcaccc cacgcactga agaaaaatac aaaaaaatta atgaagaatt
     1561 tgataatatg atcaagagtc ataaaattcc tgctgttcca cctcccaact tcgagatgcc
     1621 agtctccatc ccagtgtcca gccacaacag tttggtgtac agcaaccctg tcagctcact
     1681 gggaaacccc aacctattgc cactggctca cccttctctg cagaggaata gtatgtctcc
     1741 tggtgtaaca catcgacctc caagtgcagg taacacaggt ggtctgatgg gtggagacct
     1801 cacgtctggt gcaggcacca gtgcagggaa cgggtatggc aatccccgaa actcaccagg
     1861 tctgctggtc tcacctggta acttgaacaa gaatatgcaa gcaaaatctc ctcccccaat
     1921 gaatttagga atgaataacc gtaaaccaga tctccgagtt cttattccac caggcagcaa
     1981 gaatacgatg ccatcagtga atcaaaggat aaataactcc cagtcggctc agtcattggc
     2041 taccccagtg gtttccgtag caactcctac tttaccagga caaggaatgg gaggatatcc
     2101 atcagccatt tcaacaacat atggtaccga gtactctctg agtagtgcag acctgtcatc
     2161 tctgtctggg tttaacaccg ccagcgctct tcaccttggt tcagtaactg gctggcaaca
     2221 gcaacaccta cataacatgc caccatctgc cctcagtcag ttgggagctt gcactagcac
     2281 tcatttatct cagagttcaa atctctccct gccttctact caaagcctca acatcaagtc
     2341 agaacctgtt tctcctccta gagaccgtac caccacccct tcgagatacc cacaacacac
     2401 gcgccacgag gcggggagat ctcctgttga cagcttgagc agctgtagca gttcgtacga
     2461 cgggagcgac cgagaggatc accggaacga attccactcc cccattggac tcaccagacc
     2521 ttcgccggac gaaagggaaa gtccctcagt caagcgcatg cgactttctg aaggatgggc
     2581 aacatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2641 ttgtggaaag gacgatccat aagtgattag gtggatctaa cgcgttaagt cgacaatcaa
     2701 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2761 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2821 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2881 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2941 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3001 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3061 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3121 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3181 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3241 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3301 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3361 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3421 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3481 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3541 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3601 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3661 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3781 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3901 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3961 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4021 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4081 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4141 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4381 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4501 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4561 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4621 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4681 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4741 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4801 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4861 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4921 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4981 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5041 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5101 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5161 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5221 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5281 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5341 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5401 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5461 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5521 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5581 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5641 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5701 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6421 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6481 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6541 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6601 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6661 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6721 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6781 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6841 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6901 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6961 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7021 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7261 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7321 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7381 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7441 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7501 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7561 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7621 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7681 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7741 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7801 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7861 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7921 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7981 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8041 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8101 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8161 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8221 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8281 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8341 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8401 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8461 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8521 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8581 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8641 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8701 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8761 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8821 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8881 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8941 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9001 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9061 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9121 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9181 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9241 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9301 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9361 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9421 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9481 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9541 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9601 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9661 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9721 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
